Importance of expression system in the production of unnatural recombinant proteins in Escherichia coli

Biotechnology and Bioprocess Engineering

Volumn 14, Issue 3, 2009, Pages 257-265

  Ayyadurai, Niraikulam ^a,d   Neelamegam, Rameshkumar ^a   Nagasundarapandian, Soundrarajan ^a   Edwardraja, Selvakumar ^a   Park, Hyung Soon ^b   Lee, Soo Jae ^b   Yoo, Tae Hyeon ^c   Yoon, Hyungdon ^d   Lee, Sun Gu ^a  

^a Pusan National University   (South Korea)

^b Konkuk University   (South Korea)

^c The University of Texas at Austin   (United States)

^d Yeungnam University   (South Korea)

Author keywords

Expression system; L homopropargylglycine; Residue specific unnatural amino acid incorporation; Unnatural recombinant protein

Indexed keywords

ACTIVATION RATES; EXPRESSION SYSTEM; GREEN FLUORESCENT PROTEIN; L-HOMOPROPARGYLGLYCINE; LC-MS/MS; METHIONINE RESIDUES; NATURAL PROTEINS; OVER-EXPRESSION; PRODUCTION YIELD; RECOMBINANT PROTEIN; RESIDUE SPECIFIC UNNATURAL AMINO ACID INCORPORATION; SINGLE-CHAIN FV; SYNTHETASE; UNNATURAL RECOMBINANT PROTEIN;

AMINATION; AMINES; AMINO ACIDS; ESCHERICHIA COLI; GENE EXPRESSION; ORGANIC ACIDS; PRODUCTIVITY;

PROTEINS;

AMINO ACID DERIVATIVE; ESCHERICHIA COLI PROTEIN; GREEN FLUORESCENT PROTEIN; METHIONINE DERIVATIVE; METHIONINE TRANSFER RNA LIGASE; PROPARGYLGLYCINE; PROTEIN ANTIBODY; RECOMBINANT PROTEIN; SCATTER FACTOR RECEPTOR; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY;

ANALYTIC METHOD; ARTICLE; ENZYME ACTIVATION; GENE EXPRESSION SYSTEM; GENE OVEREXPRESSION; PROTEIN FOLDING; PROTEIN PROTEIN INTERACTION; PROTEIN SYNTHESIS;

ESCHERICHIA COLI;

EID: 67651098661     PISSN: 12268372     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12257-009-0009-z     Document Type: Article

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