Expression of recombinant chinese bovine enterokinase catalytic subunit in P. pastoris and its purification and characterization

Acta Biochimica et Biophysica Sinica

Volumn 36, Issue 7, 2004, Pages 513-517

  Fang, Lei ^a   Sun, Qi Ming ^a   Hua, Zi Chun ^a  

^a NANJING UNIVERSITY   (China)

Author keywords

Fusion protein cleavage; Ni2+ affinity chromatography; Recombinant enterokinase; Secreted expression

Indexed keywords

COMPLEMENTARY DNA; ENTEROPEPTIDASE; HISTIDINE; HYBRID PROTEIN; METHANOL; NICKEL; PROTEIN SUBUNIT; PROTEINASE; RECOMBINANT ENZYME;

AFFINITY CHROMATOGRAPHY; ARTICLE; BACTERIAL STRAIN; CATALYSIS; ENZYME ACTIVE SITE; ENZYME RELEASE; FERMENTATION; GENE SEQUENCE; GENETIC CODE; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; PICHIA PASTORIS; PLASMID; POLYMERASE CHAIN REACTION; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN PURIFICATION; SACCHAROMYCES CEREVISIAE; SIGNAL TRANSDUCTION;

BACTERIA (MICROORGANISMS); BOVINAE; PICHIA; PICHIA PASTORIS; SACCHAROMYCES; SACCHAROMYCES CEREVISIAE;

EID: 4444240985     PISSN: 05829879     EISSN: None     Source Type: Journal    
DOI: 10.1093/abbs/36.7.513     Document Type: Article

References (10)

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