Use of an acid-labile surfactant as an SDS substitute for gel electrophoresis and proteomic analysis

Journal of Biomolecular Techniques

Volumn 13, Issue 1, 2002, Pages 1-4

  Zeller, Martin ^a   Brown, Elizabeth K ^b   Bouvier, Edouard S P ^b   König, Simone ^a  

^a INST OF REPRO MED OF THE UNIV   (Germany)

^b WATERS CORPORATION   (United States)

Author keywords

Acid labile surfactant; In gel digest; Mass spectrometry; Proteomics; SDS PAGE

Indexed keywords

EID: 84856673641     PISSN: 15240215     EISSN: 19434731     Source Type: Journal    
DOI: None     Document Type: Article

References (6)

1. Shevchenko A, Wilm M, Vorm O, Mann M. Mass spectrometric sequencing of proteins from silver-stained polyacrylamide gels. Anal Chem 1996;68:850-858.
2. Zhang X, Herring CJ, Romano PR, et al. Identification of phosphorylation sites in proteins separated by polyacrylamide gel electrophoresis. Anal Chem 1998;70: 2050-2059.
3. Brown EK, Ding J, Bouvier ESP, et al. A comparison of PAGE and MS analysis of proteins and peptides in the presence of SDS or a novel acid-labile surfactant. Poster presented at the 19th International Symposium on the Separation of Proteins, Peptides, and Polynucleotides (ISPPP '99), Delray Beach, FL, October 31-November 3, 1999.
4. Compton BJ, Lee J, Brown EK, et al. A novel SDS analog compatible with MS analysis of proteins and peptides. Poster presented at the 47th Conference on Mass Spectrometry and Allied Topics, Dallas, TX, June 13-18, 1999. Poster MPI-243.
5. Zeller M, Peter-Katalinic J, Bouvier ESP, König S. Use of an acid-labile surfactant as SDS substitute for gel electrophoresis and digest analysis. Poster presented at the 49th Conference on Mass Spectrometry and Allied Topics, Chicago, IL, May 27-31, 2001. Poster ThPF-133.
6. Ross ARS, Smith D, Langridge JI, Gaskell SJ. Evaluation of a SDS analogue for 2-D PAGE and MALDI/TOF MS mapping of proteins using automated spot cutting and in-gel digestion procedures. Poster presented at the 48th Conference on Mass Spectrometry and Allied Topics, Long Beach, CA, June 11-15, 2000. Poster WPF-212.