Evaluation of a real-time PCR assay for the diagnosis of Pneumocystis pneumonia

Kurume Medical Journal

Volumn 55, Issue 3-4, 2008, Pages 55-62

  Etoh, Kohju ^a  

^a KURUME UNIVERSITY SCHOOL OF MEDICINE   (Japan)

Author keywords

BAL; GAPDH; Normalization; PCP; Realtime PCR

Indexed keywords

BACTERIAL DNA; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE;

ADULT; AGED; ARTICLE; CLINICAL ARTICLE; CONTROLLED STUDY; DIAGNOSTIC VALUE; FEMALE; HUMAN; INFANT; LUNG LAVAGE; MALE; PNEUMOCYSTIS JIROVECI; PNEUMOCYSTIS PNEUMONIA; QUALITATIVE ANALYSIS; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; SPUTUM CULTURE;

EID: 67650541400     PISSN: 00235679     EISSN: 18812090     Source Type: Journal    
DOI: 10.2739/kurumemedj.55.55     Document Type: Article

References (33)

1. Atzori C, Agostoni F, Angeli E, Mainini A, and Orlando G. Combined use of blood and oropharyngeal samples for noninvasive diagnosis of Pneumocystis carinii pneumonia using the polymerase chain reaction. Eur J Clin Microbiol Infect Dis 1998; 17:241-246.
2. Caliendo AM, Hewitt PL, Allega JM, Keen A, Ruoff KL et al. Performance of a PCR assay for detection of Pneumocystis carinii from respiratory specimens. J Clin Microbiol 1998; 36:979-982.
3. Cartwright CP, Nelson NA, and Gill VJ. Development and evaluation of a rapid and simple procedure for detection of Pneumocystis carinii by PCR. J Clin Microbiol 1994; 32:1634-1638.
4. Chouaid C, Roux P, Lavard I, Poirot JL, and Housset B. Use of the polymerase chain reaction technique on induced-sputum samples for the diagnosis of Pneumocystis carinii pneumonia in HIV-infected patients. A clinical and cost-analysis study. Am J Clin Pathol 1995; 104:72-75.
5. Flori P, Bellete B, Durand F, Raberin H, Cazorla C et al. Comparison between real-time PCR, conventional PCR and different staining techniques for diagnosing Pneumocystis jiroveci pneumonia from bronchoalveolar lavage specimens. J Med Microbiol 2004; 53:603-607.
6. Geckler RW, Gremillion DH, McAllister CK, and Ellenbogen C. Microscopic and bacteriological comparison of paired sputa and transtracheal aspirates. J Clin Microbiol 1977; 6:396-399.
7. Hauser PM, Blanc DS, Bille J, Nahimana A, and Francioli P. Carriage of Pneumocystis carinii by immunosuppressed patients and molecular typing of the organisms. Aids 2000; 14:461-463.
8. Helweg-Larsen J, Jensen JS, Benfield T, Svendsen UG, Lundgren JD et al. Diagnostic use of PCR for detection of Pneumocystis carinii in oral wash samples. J Clin Microbiol 1998; 36:2068-2072.
9. Helweg-Larsen J, Jensen JS, Dohn B, Benfield TL, and Lundgren B. Detection of Pneumocystis DNA in samples from patients suspected of bacterial pneumonia- a case-control study. BMC Infect Dis 2002; 2:28.
10. Hirooka T, Higuchi T, Tanaka N, and Ogura T. [The value of proper sputum collection instruction in detection of acid-fast bacillus]. Kekkaku 2004; 79:33-37. (in Japanese)
11. Honda J, Hoshino T, Natori H, Tokisawa S, Akiyoshi H et al. Rapid and sensitive diagnosis of cytomegalovirus and Pneumocystis carinii pneumonia in patients with haematological neoplasia by using capillary polymerase chain reaction. Br J Haematol 1994; 86:138-142.
12. l2 Huang SN, Fischer SH, O'Shaughnessy E, Gill VJ, Masur H, et al. Development of a PCR assay for diagnosis of Pneumocystis carinii pneumonia based on amplification of the multicopy major surface glycoprotein gene family. Diagn Microbiol Infect Dis 1999; 35:27-32.
13. Kovacs JA, Ng VL, Masur H, Leoung G, Hadley WK et al. Diagnosis of Pneumocystis carinii pneumonia: improved detection in sputum with use of monoclonal antibodies. N Engl J Med 1988; 318:589-593.
14. Larsen HH, Masur H, Kovacs JA, Gill VJ, Silcott VA et al. Development and evaluation of a quantitative, touch-down, real-time PCR assay for diagnosing Pneumocystis carinii pneumonia. J Clin Microbiol 2002; 40:490-494.
15. Leigh TR, Kangro HO, Gazzard BG, Jeffries DJ, and Collins JV. DNA amplification by the polymerase chain reaction to detect sub-clinical Pneumocystis carinii colonization in HIV-positive and HIV-negative male homosexuals with and without respiratory symptoms. Respir Med 1993; 87:525-529.
16. Leigh TR, Wakefield AE, Peters SE, Hopkin JM, and Collins JV. Comparison of DNA amplification and immunofluorescence for detecting Pneumocystis carinii in patients receiving immunosuppressive therapy. Transplantation 1992; 54:468-470.
17. Lipschik GY, Gill VJ, Lundgren JD, Andrawis VA, Nelson NA et al. Improved diagnosis of Pneumocystis carinii infection by polymerase chain reaction on induced sputum and blood. Lancet 1992; 340:203-206.
18. Maskell NA, Waine DJ, Lindley A, Pepperell JC, Wakefield AE et al. Asymptomatic carriage of Pneumocystis jiroveci in subjects undergoing bronchoscopy: a prospective study. Thorax 2003; 58:594-597.
19. Nevez G, Raccurt C, Jounieaux V, Dei-Cas E, and Mazars E. Pneumocystosis versus pulmonary Pneumocystis carinii colonization in HIV-negative and HIV-positive patients. Aids 1999; 13:535-536.
20. Olsson M, Elvin K, Lofdahl S, and Linder E. Detection of Pneumocystis carinii DNA in sputum and bronchoalveolar lavage samples by polymerase chain reaction. J Clin Microbiol 1993; 31:221-226.
21. Rabodonirina M, Raffenot D, Cotte L, Boibieux A, Mayencon M et al. Rapid detection of Pneumocystis carinii in bronchoalveolar lavage specimens from human immunodeficiency virus-infected patients: use of a simple DNA extraction procedure and nested PCR. J Clin Microbiol 1997; 35:2748-2751.
22. Ribes JA, Limper AH, Espy MJ, and Smith TF. PCR detection of Pneumocystis carinii in bronchoalveolar lavage specimens: analysis of sensitivity and specificity. J Clin Microbiol 1997; 35:830-835.
23. Roux P, Lavrard I, Poirot JL, Chouaid C, Denis M et al. Usefulness of PCR for detection of Pneumocystis carinii DNA. J Clin Microbiol 1994; 32:2324-2326.
24. Sing A, Roggenkamp A, Autenrieth IB, and Heesemann J. Pneumocystis carinii carriage in immunocompetent patients with primary pulmonary disorders as detected by single or nested PCR. J Clin Microbiol 1999; 37:3409-3410.
25. Sing A, Trebesius K, Roggenkamp A, Russmann H, Tybus K et al. Evaluation of diagnostic value and epidemiological implications of PCR for Pneumocystis carinii in different immunosuppressed and immunocompetent patient groups. J Clin Microbiol 2000; 38:1461-1467.
26. Skot J, Lerche AG, Kolmos HJ, Nielsen JO, Mathiesen LR et al. Pneumocystis carinii in bronchoalveolar lavage and induced sputum: detection with a nested polymerase chain reaction. Scand J Infect Dis 1995; 27:363-367.
27. Takahashi T, Goto M, Endo T, Nakamura T, Yusa N et al. Pneumocystis carinii carriage in immunocompromised patients with and without human immunodeficiency virus infection. J Med Microbiol 2002; 51:611-614.
28. Torres J, Goldman M, Wheat LJ, Tang X, Bartlett MS et al. Diagnosis of Pneumocystis carinii pneumonia in human immunodeficiency virus-infected patients with polymerase chain reaction: a blinded comparison to standard methods. Clin Infect Dis 2000; 30:141-145.
29. Wakefield AE, Guiver L, Miller RF, and Hopkin JM. DNA amplification on induced sputum samples for diagnosis of Pneumocystis carinii pneumonia. Lancet 1991; 337:1378-1379.
30. Wakefield AE, Miller RF, Guiver LA, and Hopkin JM. Oropharyngeal samples for detection of Pneumocystis carinii by DNA amplification. Q J Med 1993; 86:401-406.
31. Wakefield AE, Pixley FJ, Banerji S, Sinclair K, Miller RF et al. Detection of Pneumocystis carinii with DNA amplification. Lancet 1990; 336:451-453.
32. Weig M, Klinker H, Bogner BH, Meier A, and Gross U. Usefulness of PCR for diagnosis of Pneumocystis carinii pneumonia in different patient groups. J Clin Microbiol 1997; 35:1445-1449.
33. Zaman MK, Wooten OJ, Suprahmanya B, Ankobiah W, Finch PJ et al. Rapid noninvasive diagnosis of Pneumocystis carinii from induced liquefied sputum. Ann Intern Med 1988; 109:7-10.