Performance of a PCR assay for detection of Pneumocystis carinii from respiratory specimens

Journal of Clinical Microbiology

Volumn 36, Issue 4, 1998, Pages 979-982

  Caliendo, Angela M ^a,b   Hewitt, Peter L ^c   Allega, Jessica M ^a,b   Keen, Anne ^c   Ruoff, Kathryn L ^a,b   Ferraro, Mary Jane ^a,b  

^a MASSACHUSETTS GENERAL HOSPITAL   (United States)

^b HARVARD MEDICAL SCHOOL   (United States)

^c HOFFMANN LA ROCHE INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BACTERIUM DETECTION; DIAGNOSTIC ACCURACY; FLUORESCENT ANTIBODY TECHNIQUE; HUMAN; LUNG LAVAGE; MAJOR CLINICAL STUDY; PERFORMANCE; PNEUMOCYSTIS PNEUMONIA; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SCREENING TEST; SPUTUM ANALYSIS;

PNEUMOCYSTIS CARINII;

EID: 0031896968     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/jcm.36.4.979-982.1998     Document Type: Article

References (12)

1. Barlett, M. S., and J. W. Smith. 1991. Pneumocystis carinii, an opportunist in immunocompromised patients. Clin. Microbiol. Rev. 4:137-149.
2. Cartwright, C. P., N. A. Nelson, and V. J. Gill. 1994. Development and evaluation of a rapid and simple procedure for detection of Pneumocystis carinii by PCR. J. Clin. Microbiol. 32:1634-1638.
3. Centers for Disease Control. 1992. Recommendations for prophylaxis against Pneumocystis carinii pneumonia for adults and adolescents infected with HIV. JAMA 267:2294-2296, 2298-2299.
4. Chouaid, C., P. Roux, I. Lavard, J. L. Poirot, and B. Housset. 1995. Use of the polymerase chain reaction technique on induced-sputum samples for the diagnosis of Pneumocystis carinii pneumonia in HIV-infected patients. Am. J. Clin. Pathol. 104:72-75.
5. Elvin, K., M. Olsson, C. Lidman, and A. Bjorkman. 1996. Detection of asymptomatic Pneumocystis carinii infection by polymerase chain reaction: predictive for subsequent pneumonia. AIDS 10:1296-1297.
6. Hadley, W. K., and V. L. Ng. 1995. Pneumocysits, p. 738-748. In P. R. Murray, E. J. Barron, M. A. Pfaller, F. C. Tenover, and R. H. Yolken (ed.), Manual of clinical microbiology, 6th ed. ASM Press, Washington, D.C.
7. Kovacs, J., J. Hiemenz, A. M. Macher, et al. 1983. A comparison of Pneumocystis carinii pneumonia in patients with and without the acquired immunodeficiency syndrome. Clin. Res. 31:691A.
8. Kovacs, J. A., V. L. Ng, H. Masur, G. Leoung, W. K. Hadley, G. Evens, H. C. Lane, F. P. Ognibene, J. Shelhamer, J. E. Parrillo, and V. J. Gill. 1988. Diagnosis of Pneumocystis carinii pneumonia: improved detection in sputum with use of monoclonal antibodies. N. Engl. J. Med. 318:589-593.
9. Lipschik, G. Y., V. J. Gill, J. D. Lundgren, V. A. Andrawis, N. A. Nelson, J. O. Nielsen, F. P. Ognibene, and J. A. Kovacks. 1992. Improved diagnosis of Pneumocystis carinii infection by polymerase chain reaction on induced sputum and blood. Lancet 340:203-206.
10. Ribes, J. A., A. H. Limper, M. J. Espy, and T. F. Smith. 1997. PCR detection of Pneumocystis carinii in bronchoalveolar lavage specimens: analysis of sensitivity and specificity. J. Clin. Microbiol. 35:830-835.
11. Roux, P., I. Lavrard, J. L. Poirot, C. Chouaid, M. Denis, J. L. Oliver, M. Nigou, and M. Miltgen. 1994. Usefulness of PCR for detection of Pneumocystis carinii DNA. J. Clin. Microbiol. 32:2324-2326.
12. Selik, R. M., E. T. Starcher, J. W. Curran. 1987. Opportunistic diseases reported in AIDS patients: frequencies, associations, and trends. AIDS 1:175-182.