Rapid detection of Pneumocystis carinii in bronchoalveolar lavage specimens from human immunodeficiency virus-infected patients: Use of a simple DNA extraction procedure and nested PCR

Journal of Clinical Microbiology

Volumn 35, Issue 11, 1997, Pages 2748-2751

  Rabodonirina, Meja ^a   Raffenot, Didier ^a   Cotte, Laurent ^b   Boibieux, André ^c   Mayençon, Martine ^a   Bayle, Germaine ^a   Persat, Florence ^a   Rabatel, Fabien ^b   Trepo, Christian ^b   Peyramond, Dominique ^c   Piens, Marie Antoinette ^a  

^a UNIVERSITÉ LYON 1   (France)

^b HÔTEL DIEU   (France)

^c CROIX ROUSSE HOSPITAL   (France)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIINFECTIVE AGENT; CHLOROFORM; COTRIMOXAZOLE; METHYLAMINE; PHENOL; PROTEINASE K; PROTOZOAL DNA;

ARTICLE; CHEMOPROPHYLAXIS; CLINICAL PROTOCOL; CONTROLLED STUDY; DNA DETERMINATION; FEMALE; GIEMSA STAIN; HUMAN; HUMAN CELL; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; HUMAN TISSUE; LUNG LAVAGE; MAJOR CLINICAL STUDY; MALE; OPEN LUNG BIOPSY; PNEUMOCYSTIS CARINII; PNEUMOCYSTIS PNEUMONIA; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RESPIRATORY TRACT INFECTION; SILVER STAINING; SPUTUM SMEAR;

ACRONICTA LEPORINA; HUMAN IMMUNODEFICIENCY VIRUS; PNEUMOCYSTIS CARINII;

EID: 0030871012     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/jcm.35.11.2748-2751.1997     Document Type: Article

References (40)

1. Amicosante, M., L. Richeldi, G. Trenti, G. Paone, M. Campa, A. Bisetti, and C. Saltini. 1995. Inactivation of polymerase inhibitors for Mycobacterium tuberculosis DNA amplification in sputum by using capture resin. J. Clin. Microbiol. 33:629-630.
2. Borensztein, L., I. Hatin, A. M. Simonpoli, E. Ugarte, P. M. Girard, and G. Jaureguiberry. 1992. An alternative to DNA extraction for the diagnosis of Pneumocystis carinii pneumonia by polymerase chain reaction using a new oligonucleotide probe. Mol. Cell. Probes 6:361-365.
3. Cartwright, C. P., N. A. Nelson, and V. J. Gill. 1994. Development and evaluation of a rapid and simple procedure for detection of Pneumocystis carinii by PCR. J. Clin. Microbiol. 32:1634-1638.
4. Contini, R. Romani, V. Vullo, S. Delia, and F. Sorice. 1992. Does laboratory recovery of Pneumocystis carinii always mean clinically significant disease? AIDS 6:1558-1559.
5. De Luca, A., E. Tamburrini, E. Ortona, P. Mancarini, P. Margutti, A. Antinori, E. Visconti, and A. Siracusano. 1995. Variable efficiency of three primer pairs for the diagnosis of Pneumocystis carinii pneumonia by the polymerase chain reaction. Mol. Cell. Probes 9:330-340.
6. Elvin, K., C. Lidman, E. Tynell, E. Linder, and A. Björkman. 1994. Natural history of asymptomatic and symptomatic Pneumocystis carinii infection in HIV infected patients. Scand. J. Infect. Dis. 26:643-651.
7. Elvin, K., M. Olsson, C. Lidman, and A. Bjorkman. 1996. Detection of asymptomatic infection by polymerase chain reaction: predictive for subsequent pneumonia. AIDS 10:1296-1297.
8. Epstein, L. J., R. D. Meyer, S. Antonson, S. M. Strigle, and Z. Mohsenifar. 1994. Persistence of Pneumocystis carinii in patients with AIDS receiving chemoprophylaxis. Am. J. Respir. Crit. Care Med. 150:1456-1459.
9. Evans, R., A. Joss, D. Ho-Yen, and K. F. Whyte. 1995. Routine diagnosis of Pneumocyslis carinii pneumonia. J. Clin. Pathol. 48:91-92.
10. Evans, R., A. W. L. Joss, D. Parratt, T. H. Pennington, and D. O. Ho-Yen. 1995. The role of a nested polymerase chain reaction in the diagnosis of Pneumocystis carinii pneumonia. J. Clin. Pathol. Mol. Pathol. 48:M347-M350.
11. Evans, R., A. W. L. Joss, T. H. Pennington, and D. O. Ho-Yen. 1995. The use of a nested polymerase chain reaction for detecting Pneumocystis carinii from lung and blood in rat and human infection. J. Med. Microbiol. 42:209-213.
12. Gryzan, S., I. L. Paradis, A. Zeevi, R. J. Duquesnoy, J. S. Dummer, B. P. Griffith, R. L. Hardesty, A. Trento, M. A. Nalesnik, and J. H. Dauber. 1988. Unexpectedly high incidence of Pneumocystis carinii infection after lung-heart transplantation. Am. Rev. Respir. Dis. 137:1268-1274.
13. Huang, L., F. M. Hecht, J. F. Gruden, K. Kearns, J. Turner, J. D. Stansell, and P. C. Hopewell. 1996. Predicting Pneumocystis carinii pneumonia: results of a multivariate analysis, abstr. Tu. B. 110, p. 229. In Abstracts of the XI International Conference on AIDS.
14. Hugues, W. T. 1979. Limited effect of trimethoprim-sulfamethoxazole prophylaxis on Pneumocystis carinii. Antimicrob. Agents Chemother. 16:333-335.
15. Innis, M. A., and D. H. Gelfand. 1990. Optimization of PCRs, p. 3-12. In M. A. Innis, D. H. Gelfand, J. J. Sninsky, and T. J. White (ed.), PCR protocols: a guide to methods and applications. Academic Press, London, United Kingdom.
16. Jules-Elysee, K. M., D. E. Stover, M. B. Zaman, E. M. Bernard, and D. A. White. 1990. Aerosolized pentamidine: effect on diagnosis and presentation of Pneumocystis carinii pneumonia. Ann. Intern. Med. 112:750-757.
17. Leibovitz, E., H. Pollack, T. Moore, J. Papellas, L. Gallo, K. Krasinski, and W. Borkowsky. 1995. Comparison of PCR and standard cytological staining for detection of Pneumocystis carinii from respiratory specimens from patients with or at high risk for infection by human immunodeficiency virus. J. Clin. Microbiol. 33:3004-3007.
18. Leigh, T. R., B. G. Gazzard, A. Rowbotton, and J. V. Collins. 1993. Quantitative and qualitative comparison of DNA amplification by PCR with immunofluorescence staining for diagnosis of Pneumocystis carinii pneumonia. J. Clin. Pathol. 46:140-144.
19. Leigh, T. R., H. O. Kangro, B. G. Gazzard, D. J. Jeffries, and J. V. Collins. 1993. DNA amplification by the polymerase chain reaction to detect subclinical Pneumocystis carinii colonization in HIV-positive and HIV-negative homosexuals with and without respiratory symptoms. Respir. Med. 87:525-529.
20. Leigh, T. R., A. E. Wakefield, S. E. Peters, J. M. Hopkin, and J. V. Collins. 1992. Comparison of DNA amplification and immunofluorescence for detecting Pneumocystis carinii in patients receiving immunosuppressive therapy. Transplantation 54:468-470.
21. Levine, S. J., D. Kennedy, J. H. Shelhamer, A. Kovacs, I. M. Feuerstein, V. J. Gill, F. Stock, D. Solomon, C. T. Boylen, H. Masur, and F. P. Ognibene. 1992. Diagnosis of Pneumocystis carinii pneumonia by multiple lobe, site-directed bronchoalveolar lavage with immunofluorescent monoclonal antibody staining in human immunodeficiency virus-infected patients receiving aerosolized pentamidine chemoprophylaxis. Am. Rev. Respir. Dis. 146:838-843.
22. Lipschick, G. Y., V. J. Gill, J. D. Lungren, V. A. Andrawis, N. A. Nelson, J. O. Nielsen, F. P. Ognibene, and J. A. Kovacs. 1992. Improved diagnosis of Pneumocystis carinii infection by polymerase chain reaction on induced sputum and blood. Lancet 340:203-206.
23. Lu, J. J., C. H. Chen, M. S. Bartlett, J. W. Smith, and C. H. Lee. 1995. Comparison of six different PCR methods for detection of Pneumocystis carinii. J. Clin. Microbiol. 33:2785-2788.
24. Mazars, E., S. Herbecq, A. S. Szypura, J. Fruit, D. Camus, and E. Dei-Cas. 1996. Pneumocystis carinii detection in 158 HIV-seronegative patients. J. Eukaryot. Microbiol. 43:28S.
25. Moonens, F., C. Liesnard, F. Brancard, J. P. van Vooren, and E. Serruys. 1995. Rapid simple and nested polymerase chain reaction for the diagnosis of Pneumocystis carinii pneumonia. Scand. J. Infect. Dis. 27:358-362.
26. Olsson, M., K. Elvin, S. Löfdahl, and E. Linder. 1993. Detection of Pneumocystis carinii DNA in sputum and bronchoalveolar lavage samples by polymerase chain reaction. J. Clin. Microbiol. 31:221-226.
27. Richards, C. G., A. E. Wakefield, and C. D. Mitchell. 1994. Detection of Pneumocystis DNA in nasopharyngeal aspirates of leukaemic infants with pneumonia. Arch. Dis. Child. 71:254-255.
28. Roux, P., I. Lavrard, J. L. Poirot, C. Chouaid, M. Denis, J. L. Olivier, M. Nigou, and M. Miltgen. 1994. Usefulness of PCR for detection of Pneumocystis carinii DNA. J. Clin. Microbiol. 32:2324-2326.
29. Savoia, D., M. Millesimo, I. Ricatto, R. Fora, M. T. Brusa, I. Cassetta, T. Brancale, B. Forno, C. Zamprogna, and P. Caramello. 1996. Nested PCR for the diagnosis of Pneumocystis carinii pneumonia in HIV-positive patients, abstr. E10.23, p. 422. In Abstracts of the VII European Multicolloquium of Parasitology.
30. Schluger, N., T. Godwin, K. Sepkowitz, D. Armstrong, E. Bernard, M. Rifkin, A. Cerami, and R. Bucala. 1992. Application of DNA amplification to pneumocystosis: presence of serum Pneumocystis carinii DNA during human and experimentally induced Pneumocystis carinii pneumonia. J. Exp. Med. 176: 1327-1333.
31. Sepkowitz, K., N. Schluger, T. Godwin, D. Armstrong, A. Cerami, and R. Bucala. 1993. DNA amplification in experimental pneumocystosis: characterization of serum Pneumocystis carinii DNA and potential P. carinii carrier states. J. Infect. Dis. 168:421-426.
32. Skot, J., A. G. Lerche, H. J. Kolmos, J. O. Nielsen, L. R. Mathiesen, and J. D. Lungren. 1995. Pneumocystis carinii in bronchoalveolar lavage and induced sputum: detection with a nested polymerase chain reaction. Scand. J. Infect. Dis. 27:363-367.
33. Tamburrini, E., P. Mancarini, A. de Luca, A. Antinori, E. Visconti, A. Ammassari, L. Ortona, A. Siracusano, E. Ortona, and G. Vicari. 1993. Simple and rapid two-step polymerase chain reaction for the diagnosis of Pneumocystis carinii infection. J. Clin. Microbiol. 31:2788-2789.
34. Tamburrini, E., P. Mancarini, A. de Luca, G. Maiuro, G. Ventura, A. Antinori, A. Ammassari, E. Visconti, L. Ortona, A. Siracusano, E. Ortona, and G. Vicari. 1993. Diagnosis of Pneumocystis carinii pneumonia: specificity and sensitivity of polymerase chain reaction in comparison of immunofluorescence in bronchoalveolar lavage specimens. J. Med. Microbiol. 38:449-453.
35. Vargas, S. L., W. T. Hugues, A. E. Wakefield, and H. S. Oz. 1995. Limited persistence in and subsequent elimination of Pneumocystis carinii from the lungs after P. carinii pneumonia. J. Infect. Dis. 172:506-510.
36. Wakefield, A. E., L. Guiver, R. F. Miller, and J. M. Hopkin. 1991. DNA amplification on induced sputum samples for diagnosis of Pneumocystis carinii pneumonia. Lancet 337:1378-1379.
37. Wakefield, A. E., R. F. Miller, L. A. Guiver, and J. M. Hopkin. 1993. Oropharyngeal samples for detection of Pneumocystis carinii by DNA amplification. Q. J. Med. 86:401-406.
38. Wakefield, A. E., F. J. Pixley, S. Banerji, K. Sinclair, R. F. Miller, E. R. Moxon, and J. M. Hopkin. 1990. Detection of Pneumocystis carinii with DNA amplification. Lancet 336:451-453.
39. Wakefield, A. E., F. J. Pixley, S. Banerji, K. Sinclair, R. F. Miller, E. R. Moxon, and J. M. Hopkin. 1990. Amplification of mitochondrial ribosomal RNA sequences from Pneumocystis carinii DNA of rat and human origin. Mol. Biochem. Parasitol. 43:69-76.
40. Weig, M., H. Klinker, M. Wilhem, K. Lemmer, and U. Gross. 1996. Correlation of Pneumocystis carinii PCR with clinical diagnosis in immunocompromised patients. Lancet 347:1266.