Diagnostic use of PCR for detection of Pneumocystis carinii in oral wash samples

Journal of Clinical Microbiology

Volumn 36, Issue 7, 1998, Pages 2068-2072

  Helweg Larsen, Jannik ^a   Jensen, Jørgen Skov ^b   Benfield, Thomas ^a   Svendsen, Ulrik Gerner ^c   Lundgren, Jens D ^a   Lundgren, Bettina ^a,d  

^a HVIDOVRE HOSPITAL   (Denmark)

^b Statens Seruminstitut   (Denmark)

^c Rigshospitalet   (Denmark)

^d Department of Clinical Microbiology ^*  (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BRONCHOSCOPY; DIAGNOSTIC ACCURACY; DIAGNOSTIC VALUE; HUMAN; LUNG LAVAGE; MAJOR CLINICAL STUDY; MOUTHWASH; NONHUMAN; PARASITE IDENTIFICATION; PNEUMOCYSTIS CARINII; PNEUMOCYSTIS PNEUMONIA; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SPUTUM ANALYSIS;

HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS 1; PNEUMOCYSTIS CARINII; PROTOZOA;

EID: 0031808153     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/jcm.36.7.2068-2072.1998     Document Type: Article

References (38)

1. Andersen, A. B., S. Thybo, G. P. Fausset, and N. G. Stoker. 1993. Polymerase chain reaction for detection of Mycobacterium tuberculosis in sputum. Eur. J. Clin. Microbiol. Infect. Dis. 12:922-927.
2. Atzori, C., F. Agostoni, G. Gubertini, and A. Cargnel. 1996. Diagnosis of PCP by ITSs nested PCR on noninvasive oropharyngeal samples. J. Eukaryot. Microbiol. 43:41S.
3. Atzori, C., J.-J. Lu, B. Jiang, M. S. Bartlett, G. Orlando, S. F. Queener, J. W. Smith, A. Cargnel, and C.-H. Lee. 1995. Diagnosis of Pneumocystis carinii pneumonia in AIDS patients by using polymerase chain reactions on serum specimens. J. Infect. Dis. 172:1623-1626.
4. Bauer, H. M., Y. Ting, C. E. Green, J. C. Chambers, C. J. Tashiro, J. Chimera, A. Reingold, and M. M. Manos. 1991. Genital human papillomavirus infection in female university students as determined by a PCR-based method. JAMA 265:472-477.
5. Calderón, E., C. Regordán, F. J. Medrano, M. Ollero, and J. M. Varela. 1996. Pneumocystis carinii infection in patients with chronic bronchial disease. Lancet 347:977.
6. Cartwright, C. P., N. A. Nelson, and V. J. Gill. 1994. Development and evaluation of a rapid and simple procedure for detection of Pneumocystis carinii by PCR. J. Clin. Microbiol. 32:1634-1638.
7. Chou, Q., M. Russel, D. E. Birch, J. Raymond, and W. Bloch. 1992. Prevention of pre-PCR mis-priming and primer dimerization improves low-copy-number amplifications. Nucleic Acids Res. 20:1717-1723.
8. Chouaid, C., P. Roux, I. Lavard, J. L. Poirot, and B. Housset. 1995. Use of polymerase chain reaction technique on induced-sputum samples for the diagnosis of Pneumocystis carinii pneumonia in HIV-infected patients. Am J. Clin. Pathol. 104:72-75.
9. Contini, C., R. Romani, M. Manganaro, F. Sorice, and S. Delia. 1993. Tissue culture isolation of Pneumocystis carinii from peripheral blood mononuclear cells of AIDS patients with PCP. AIDS 7:1137-1138.
10. Don, R. H., P. T. Cox, B. J. Wainwright, K. Baker, and J. S. Mattick. 1991. "Touchdown" PCR to circumvent spurious priming during gene amplification. Nucleic Acids Res. 19:4008.
11. Elvin, K., A. Björkman, E. Linder, N. Heurlin, and A. Hjerpe. 1988. Pneumocystis carinii pneumonia: detection of parasites in sputum and bronchoalveolar lavage fluid by monoclonal antibodies. Br. Med. J. 297:381-384.
12. Evans, R., A. W. L. Joss, T. H. Pennington, and D. O. Ho-Yen. 1995. The use of a nested polymerase chain reaction for detecting Pneumocystis carinii from lung and blood in rat and human infection. J. Med. Microbiol. 42:209-213.
13. Hecker, K. H., and K. H. Roux. 1996. High and low annealing temperatures increase both specificity and yield in Touchdown and stepdown PCR. Bio-Techniques 20:478-485.
14. Helweg-Larsen, J., J. S. Jensen, and B. Lundgren. 1997. Non-invasive diagnosis of Pneumocystis carinii pneumonia in haematological patients using PCR on oral washes. Lancet 350:1365.
15. Hunter, J. A. C., and A. E. Wakefield. 1996. Genetic divergence at the small subunit ribosomal RNA gene among isolates of Pneumocystis carinii from five mammalian host species. J. Eukaryot. Microbiol. 43:24S-25S.
16. Kovacs, J. A., V. L. Ng, H. Masur, G. Leoung, W. K. Hadley, G. Evans, F. P., H. C. Lane, F. P. Ognibene, J. Shelhamer, J. E. Parillo, and V. J. Gill. 1988. Diagnosis of Pneumocystis carinii pneumonia: improved detection of in induced sputum with use of monoclonal antibodies. N. Engl. J. Med. 318: 583-593.
17. Leibovitz, E., H. Pollack, M. Rigaud, A. Kaul, D. Persaud, L. Gallo, J. Papellas, W. Krasinski, and W. Borkowskj. 1995. Polymerase chain reaction is more sensitive than standard cytologic stains in detecting Pneumocystis carinii in bronchoalveolar lavage from human immunodeficiency virus type 1-infected infants and children with pneumonia. Pediatr. Infect. Dis. J. 14: 714-716.
18. Leigh, T. R., H. O. Kangro, B. G. Gazzard, D. J. Jefferies, and J. V. Collins. 1993. DNA amplification by polymerase chain reaction to detect subclinical Pneumocystis carinii colonization in HIV-positive and HIV-negative homosexuals with and without respiratory symptoms. Respir. Med. 87:525-529.
19. Lipschik, G. Y., V. J. Gill, J. D. Lundgren, V. A. Andrawis, N. A. Nelson, J. O. Nielsen, F. P. Ognibene, and J. A. Kovacs. 1992. Improved diagnosis of Pneumocystis carinii infection by polymerase chain reaction on induced sputum and blood. Lancet 340:203-206.
20. Lu, J.-J., C.-H. Chen, M. S. Bartlett, J. W. Smith, and C.-H. Lee. 1997. Comparison of six different PCR methods for detection of Pneumocystis carinii. J. Clin. Microbiol. 33:2785-2788.
21. Lundgren, B., J. D. Lundgren, T. Nielsen, L. Mathiesen, J. O. Nielsen, and J. A. Kovacs. 1992. Antibody responses to a major Pneumocystis carinii antigen in human immunodeficiency virus-infected patients with and without P. carinii pneumonia. J. Infect. Dis. 165:1151-1155.
22. Lundgren, J. D., S. E. Barton, A. Lazzarin, S. Danner, F. D. Goebel, P. Pehrson, F. Mulcahy, J. Kosmidis, C. Pedersen, and A. N. Phillips. 1995. Factors associated with the occurrence of Pneumocystis carinii pneumonia in 5025 European AIDS patients. AIDS in Europe Study Group. Clin. Infect. Dis. 21:106-113.
23. Mathis, A., R. Weber, H. Kuster, and R. Speich. 1997. Simplified sample processing combined with a sensitive one-tube nested PCR assay for detection of Pneumocystis carinii in respiratory specimens. J. Clin. Microbiol. 35: 1691-1695.
24. Mayer, C. L., and C. J. Palmer. 1996. Evaluation of PCR, nested PCR, and fluorescent antibodies for detection of Giardia and Cryptosporidium species in wastewater. Appl. Environ. Microbiol. 62:2081-2085.
25. Olsson, M., K. Elvin, S. Löfdahl, and E. Linder. 1993. Detection of Pneumocystis carinii DNA in sputum and bronchoalveolar lavage samples by polymerase chain reaction. J. Clin. Microbiol. 31:221-226.
26. Rabodonirina, M., D. Raffenot, L. Cotte, A. Boibieux, M. Mayencon, G. Bayle, F. Persat, F. Rabatel, C. Trepo, D. Payramond, and M.-A. Piens. 1997. Rapid detection of Pneumocystis carinii in bronchoalveolar lavage specimen from human immunodeficiency virus-infected patients: use of a simple DNA extraction procedure and nested PCR. J. Clin. Microbiol. 35:2748-2751.
27. Ribes, J. A., A. H. Limper, M. J. Espy, and T. F. Smith. 1997. PCR detection of Pneumocystis carinii in bronchoalveolar lavage specimens: analysis of sensitivity and specificity. J. Clin. Microbiol. 35:830-835.
28. Roux, P., I. Lavrard, C. Chouaid, M. Denis, J. L. Olivier, M. Nigou, and M. Miltgen. 1994. Usefulness of PCR for detection of Pneumocystis carinii DNA. J. Clin. Microbiol. 32:2324-2326.
29. Schluger, N., T. Godwin, K. Sepkowitz, D. Armstrong, E. Bernard, M. Rifkin, A. Cerami, and R. Bucala. 1992. Application of DNA amplification to pneumocystosis: presence of serum Pneumocystis carinii DNA during human and experimentally induced Pneumocystis carinii pneumonia. J. Exp. Med. 176: 1327-1333.
30. Skøt, J., A. Lerche, H. J. Kolmos, and J. D. Lundgren. 1995. Pneumocystis carinii in bronchoalveolar lavage and induced sputum: detection with nested polymerase chain reaction. Scand. J. Infect. Dis. 27:363-367.
31. Tamborini, E., P. Mencarini, A. De Luca, G. Maiuro, G. Ventura, A. Siracusano, E. Ortona, and G. Vicari. 1993. Diagnosis of Pneumocystis carinii pneumonia: specificity and sensitivity of polymerase chain reaction in comparison with immunofluorescence in bronchoalveolar lavage specimens. J. Med. Microbiol. 38:449-4.53.
32. Tamborini, E., P. Mencarini, E. Visconti, M. Zolfo, A. De Luca, A. Siracusano, E. Ortona, and A. E. Wakefleld. 1996. Detection of Pneumocystis carinii DNA in blood by PCR is not of value for diagnosis of P. carinii pneumonia. J. Clin. Microbiol. 34:1568-1588.
33. Ursi, J. P., D. Ursi, M. Ieven, and S. R. Pattyn. 1992. Utility of an internal control for the polymerase chain reaction - application to detection of mycoplasma pneumonia in clinical specimens. APMIS 100:635-639.
34. Wakefield, A. E., L. Guliver, R. F. Miller, and J. M. Hopkin. 1991. DNA amplification on induced sputum samples for diagnosis of Pneumocystis carinii pneumonia. Lancet 337:1378-1379.
35. Wakefield, A. E., R. F. Miller, L. A. Guiver, and J. M. Hopkin. 1993. Oropharyngeal samples for detection of Pneumocystis carinii by DNA amplification. Q. J. Med. 86:401-406.
36. Wakefield, A. E., F. J. Pixley, S. Banerji, K. Sinclair, R. F. Millar, E. R. Maxon, and J. M. Hopkin. 1990. Detection of Pneumocystis carinii with DNA amplification. Lancet 336:451-153.
37. Walsh, P. S., D. A. Metzger, and R. Higuchi. 1991. Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. BioTechniques 10:506-513.
38. Weig, M., H. Klinker, M. Wilhem, K. Lemmer, and U. Gross. 1996. Correlation of Pneumocystis carinii PCR with clinical diagnosis in immunocompromised patients. Lancet 347:1266.