Molecular basis of the inhibitor selectivity and insights into the feedback inhibition mechanism of citramalate synthase from Leptospira interrogans

Biochemical Journal

Volumn 421, Issue 1, 2009, Pages 133-143

  Zhang, Peng ^a,b,e   Ma, Jun ^b,c   Zhang, Zilong ^b,c   Zha, Manwu ^a   Xu, Hai ^c,f   Zhao, Guoping ^c,d   Ding, Jianping ^a  

^a INSTITUTE OF BIOCHEMISTRY AND CELL BIOLOGY   (China)

^b UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

^c INSTITUTE OF PLANT PHYSIOLOGY AND ECOLOGY   (China)

^d CHINESE NATIONAL HUMAN GENOME CENTER AT SHANGHAI   (China)

^e PRINCETON UNIVERSITY   (United States)

^f SHANDONG UNIVERSITY   (China)

Author keywords

Allosteric regulation; Crystal structure; Feedback inhibition; Mechanism; Selectivity

Indexed keywords

ACETYL-COA; ACTIVE SITE; ALLOSTERIC REGULATION; BIOCHEMICAL DATA; BIOCHEMICAL STUDIES; BIOSYNTHESIS PATHWAYS; CATALYTIC REACTIONS; CONFORMATIONAL CHANGE; CONSERVED RESIDUES; DIMER INTERFACE; END-PRODUCT; FEEDBACK INHIBITION; FUNCTIONAL UNITS; HIGH SELECTIVITY; HYDROGEN BONDING INTERACTIONS; HYDROPHOBIC POCKETS; INHIBITOR SELECTIVITY; INHIBITORY EFFECT; KINETIC METHOD; MOLECULAR BASIS; PYRUVATE; REGULATORY DOMAIN; SELECTIVITY; SIDE CHAINS; SYNTHASE; THREE-LAYER; TYPICAL PATTERNS;

AMINO ACIDS; BINDING SITES; BIOCHEMICAL ENGINEERING; BIOCHEMISTRY; CATALYSIS; ENZYMES; HYDROGEN; HYDROGEN BONDS; HYDROPHOBICITY; LAWS AND LEGISLATION; OLIGOMERS;

CRYSTAL STRUCTURE;

ACETYL COENZYME A; CITRAMALIC ACID SYNTHETASE; DIMER; ISOLEUCINE; LEUCINE; PYRUVIC ACID; SYNTHETASE; TYROSINE; UNCLASSIFIED DRUG; VALINE; (R) CITRAMALATE SYNTHASE; (R)-CITRAMALATE SYNTHASE; ACYLTRANSFERASE;

ALLOSTERISM; ALPHA HELIX; ARTICLE; BETA SHEET; CARBOXY TERMINAL SEQUENCE; CATALYSIS; CHEMICAL ANALYSIS; COMPLEX FORMATION; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME INHIBITION; ENZYME MECHANISM; FEEDBACK SYSTEM; HYDROGEN BOND; HYDROPHOBICITY; LEPTOSPIRA INTERROGANS; MUTAGENESIS; NEGATIVE FEEDBACK; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DOMAIN; AMINO ACID SEQUENCE; BINDING SITE; CHEMICAL STRUCTURE; CHEMISTRY; DRUG ANTAGONISM; ENZYMOLOGY; GENE EXPRESSION REGULATION; GENETICS; METABOLISM; MOLECULAR GENETICS; PROTEIN CONFORMATION;

LEPTOSPIRA INTERROGANS;

ACYLTRANSFERASES; AMINO ACID SEQUENCE; BINDING SITES; FEEDBACK, BIOCHEMICAL; GENE EXPRESSION REGULATION, ENZYMOLOGIC; ISOLEUCINE; LEPTOSPIRA INTERROGANS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PROTEIN CONFORMATION;

EID: 67650312585     PISSN: 02646021     EISSN: 14708728     Source Type: Journal    
DOI: 10.1042/BJ20090336     Document Type: Article

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