Improving GPX activity of selenium-containing human single-chain Fv antibody by site-directed mutation based on the structural analysis

Journal of Molecular Recognition

Volumn 22, Issue 4, 2009, Pages 293-300

  Xu, Junjie ^a   Song, Jian ^a   Yan, Fei ^a   Chu, Huiying ^a   Luo, Jixun ^a   Zhao, Yongshan ^a   Cheng, Xu ^a   Luo, Guimin ^a   Zheng, Qingchuan ^a   Wei, Jingyan ^a  

^a JILIN UNIVERSITY   (China)

Author keywords

Glutathione peroxidase; Selenium; Single chain Fv fragment; Site directed mutation; Three dimensional model

Indexed keywords

CATALYTIC ANTIBODY; GLUTATHIONE; GLUTATHIONE PEROXIDASE; SELENIUM; SELENOCYSTEINE;

AMINO ACID SEQUENCE; ARTICLE; BINDING SITE; BIOLOGY; CELL CLONE; CHEMICAL STRUCTURE; CHEMISTRY; GENETICS; HUMAN; HYDROGEN BOND; IMMUNOGLOBULIN VARIABLE REGION; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR GENETICS; MUTATION; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN SECONDARY STRUCTURE; SEQUENCE ALIGNMENT; SITE DIRECTED MUTAGENESIS; THERMODYNAMICS; WESTERN BLOTTING;

AMINO ACID SEQUENCE; ANTIBODIES, CATALYTIC; BINDING SITES; BLOTTING, WESTERN; CLONE CELLS; COMPUTATIONAL BIOLOGY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GLUTATHIONE; GLUTATHIONE PEROXIDASE; HUMANS; HYDROGEN BONDING; IMMUNOGLOBULIN VARIABLE REGION; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; MUTATION; PROTEIN STRUCTURE, SECONDARY; SELENIUM; SELENOCYSTEINE; SEQUENCE ALIGNMENT; THERMODYNAMICS;

ESCHERICHIA COLI;

EID: 67650254586     PISSN: 09523499     EISSN: 10991352     Source Type: Journal    
DOI: 10.1002/jmr.946     Document Type: Article

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