Probing the presumed catalytic triad of a selenium-containing peroxidase by mutational analysis

Zeitschrift fur Ernahrungswissenschaft

Volumn 37, Issue SUPPL. 1, 1998, Pages 118-121

  Maiorino, M ^a   Aumann, K D ^b   Brigelius Flohe R ^c   Doria, D ^d   Van Den Heuvel, J ^a   McCarthy, J ^b   Roveri, A ^d   Ursini, F ^c   Flohe L ^a  

^a UNIVERSITY OF PADOVA   (Italy)

^b HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

^c GERMAN INSTITUTE OF HUMAN NUTRITION   (Germany)

^d UNIVERSITY OF UDINE   (Italy)

Author keywords

Active site; Glutathione peroxidases; Molecular mutants; Selenium catalysis

Indexed keywords

ENZYME VARIANT; GLUTAMINE; GLUTATHIONE PEROXIDASE; SELENOCYSTEINE; TRYPTOPHAN;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; CATALYSIS; CONFERENCE PAPER; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME KINETICS; ENZYME SPECIFICITY; STRUCTURE ACTIVITY RELATION;

ANIMALS; CATALYSIS; CLONING, MOLECULAR; ESCHERICHIA COLI; GLUTAMINE; GLUTATHIONE PEROXIDASE; KINETICS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; POINT MUTATION; PROTEIN CONFORMATION; SELENIUM; SELENOCYSTEINE; SWINE; TRYPTOPHAN;

EID: 0031925596     PISSN: 0044264X     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Conference Paper

References (11)

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8. Maiorino M, Aumann KD. BrigeliusFlohé R. Doria D, van den Heuvel J. McCarthy J, Roveri A, Ursini F, Flohe L (1995) Probing the presumed catalytic triad of selenium-containing peroxidases by mutational analysis of phospholipid hydroperoxide glutathione peroxidase (PHGPx). Biol Chem Hoppe Seyler 36:651 660
9. Rocher C, Lalanne JL, Chaudière J (1992) Purification and properties of a recombinant sulphur analog of murine selenium-glutathione peroxidase. Eur J Biochem 205:955-960
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