Screening human antibody libraries against carcinoma cells by affinity purification and polymerase chain reaction

Journal of Immunological Methods

Volumn 330, Issue 1-2, 2008, Pages 44-56

  Reiersen, Herald ^a   Berntsen, Grete ^a   Stassar, Marike ^a   Cochlovius, Björn ^a  

^a Affitech AS   (Norway)

Author keywords

Cancer; Library screening; Magnetic beads; PCR; Single chain antibody

Indexed keywords

BETA ACTIN; EPITOPE; MEMBRANE ANTIGEN; MONOCLONAL ANTIBODY; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; TUMOR ANTIGEN;

ANTIBODY DETECTION; ANTIBODY LIBRARY; ANTIBODY SCREENING; ARTICLE; CARCINOMA CELL; CELL SURFACE; CELL TYPE; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; EUKARYOTIC CELL; FLUORESCENCE ACTIVATED CELL SORTING; HUMAN; HUMAN CELL; LUNG CARCINOMA; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PURIFICATION; REPORTER GENE;

ACTINS; ANTIBODIES, MONOCLONAL; ANTIBODIES, NEOPLASM; ANTIBODY SPECIFICITY; ANTIGENS, NEOPLASM; BINDING SITES, ANTIBODY; CARCINOMA; CELL LINE, TUMOR; CELL SEPARATION; CHROMATOGRAPHY, AFFINITY; ENDOTHELIAL CELLS; ENZYME-LINKED IMMUNOSORBENT ASSAY; EPITOPES; FLOW CYTOMETRY; GENES, REPORTER; HUMANS; IMMUNOGLOBULIN VARIABLE REGION; LEUKOCYTES, MONONUCLEAR; LUNG NEOPLASMS; MAGNETICS; PEPTIDE LIBRARY; POLYMERASE CHAIN REACTION;

BACTERIA (MICROORGANISMS);

EID: 38649112289     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2007.10.017     Document Type: Article

References (36)

1. Adams G.P., and Weiner L.M. Monoclonal antibody therapy of cancer. Nat. Biotechnol. 23 (2005) 1147
2. Amstutz P., Forrer P., Zahnd C., and Plückthun A. In vitro display technologies: novel developments and applications. Curr. Opin. Biotechnol. 12 (2001) 400
3. Breitling F., Dübel S., Seehaus T., Klewinghaus I., and Little M. A surface expression vector for antibody screening. Gene 194 (1991) 147
4. Burton D.R. Antibodies, viruses and vaccines. Nat. Rev., Immunol. 2 (2002) 706
5. Chien C.-T., Bartel P.L., Sternglanz R., and Fields S. The two-hybrid system: a method to identify and clone genes for proteins that interact with a proteins of interest. Proc. Natl. Acad. Sci. U. S. A. 88 (1991) 9578
6. Coia G., Pontez-Braz L., Nuttall S.D., Hudson P.J., and Irving R.A. Panning and selection of proteins using ribosome display. J. Immunol. Methods 254 (2001) 191
7. De Leij L., Helfrich W., Stein R., and Mattes M.J. SCLC-cluster-2 antibodies detect the pancarcinoma/epithelial glycoprotein EGP-2. Int. J. Cancer, Suppl. 8 (1994) 60
8. Dörsam H., Rohrbach P., Kurschner T., Kipriyanov S., Renner S., Braunagel M., Welschof M., and Little M. Antibodies to steroids from a small human naive IgM library. FEBS Lett. 414 (1997) 7
9. Emili A.Q., and Cagney G. Large-scale functional analysis using peptide or proteins arrays. Nat. Biotechnol. 18 (2000) 393
10. Flatmark K., Bjørnland K., Johannessen H.O., Hegstad E., Rosales R., Hårklau L., Solhaug J.H., Faye R.S., Søreide O., and Fodstad Ø. Immunomagnetic detection of micrometastatic cells in bone marrow of colorectal cancer patients. Clin. Cancer Res. 8 (2002) 444
11. Gee A.P., Mansour V.H., and Weiler M.B. Effects of target antigen density on the efficacy of immunomagnetic cell separation. J. Immunol. Methods 142 (1991) 127
12. Giordano R.J., Cardo-Vila M., Lahdenranta J., Pasqualini R., and Arap W. Biopanning and rapid analysis of selective interactive ligands. Nat. Med. 7 (2001) 1249
13. Harrison J.L., Williams S.C., Winter G., and Nissim A. Screening of phage display antibody libraries. Methods Enzymol. 267 (1996) 83
14. Kipriyanov S.M., Moldenhauer G., and Little M. High level production of soluble single chain antibodies in small-scale Escherichia coli cultures. J. Immunol. Methods 200 (1997) 67
15. Kourilov V., and Steinitz M. Magnetic-bead enzyme-linked immunosorbent assay verifies adsorption of ligand and epitope accessibility. Anal. Biochem. 311 (2002) 166
16. Lee S.Y., Choi J.H., and Xu Z. Microbial cell-surface display. Trends Biotechnol. 21 (2003) 45
17. Li M. Applications of display technology in protein analysis. Nat. Biotechnol. 18 (2000) 1251
18. Lopez J.G., Chew S.J., Thompson H.W., Malter J.S., Insler M.S., and Beuerman R.W. Invest. Ophthamol. Vis. Sci. 33 (1992) 2053
19. Løset G.Å., Løbersli I., Kavlie A., Stacy J.E., Borgen T., Kausmally L., Hvattum E., Simonsen B., Hovda M.B., and Brekke O.H. Construction, evaluation and refinement of a large human antibody phage library based on the IgD and IgG variable gene repertoire. J. Immunol. Methods 299 (2005) 47
20. McCafferty, J., Pope, A.R., Stuart, J.K., Hoogenboom, H.R.J., Griffiths, A.D., Jackson, R.H., Holliger, K.P., Marks, J.D., Clackson, T.P., Chiswell, D.J., Winter, G.P., Bonnert, T.P., 1992. Methods for producing members of specific binding pairs. WO 92/01047.
21. Nakano H., Kobayashi K., Ohuchi S., Sekiguchi S., and Yamane T. Single-step single-molecule PCR of DNA with a homo-priming sequence using a single primer and hot-startable DNA polymerase. J. Biosci. Bioeng. 90 (2000) 456
22. Odegrip R., Coomber D., Eldridge B., Hederer R., Kuhlman P.A., Ullman C., FitzGerald K., and McGregor K. CIS display: in vitro selection of peptides from libraries of protein-DNA complexes. Proc. Natl. Acad. Sci. U. S. A. 101 (2004) 2806
23. Ohuchi S., Nakano H., and Yamane T. In vitro method for the generation of protein libraries using PCR amplification of a single DNA molecule and coupled transcription/translation. Nucleic Acids Res. 26 (2000) 4339
24. Patel D., and Rickwood D. Optimization of conditions for specific binding of antibody-coated beads to cells. J. Immunol. Methods 184 (1995) 71
25. Reiersen H., Løbersli I., Løset G.Å., Hvattum E., Simonsen B., Stacy J.E., McGregor D., FitzGerald K., Welschof M., Brekke O.H., and Marvik O.J. Covalent antibody library - an in vitro antibody-DNA library selections system. Nucleic Acids Res. 33 (2005) e10
26. Samuelson P., Gunneriusson E., Nygren P.-Å., and Ståhl S. Display of proteins on bacteria. J. Biotechnol. 96 (2002) 129
27. Schaffitzel C., Hanes J., Jermutus L., and Plückthun A. Ribosome display: an in vitro method for selection and evolution of antibodies from libraries. J. Immunol. Methods 231 (1999) 119
28. Stacy J.E., Kausmally L., Simonsen B., Nordgard S.H., Alsøe L., Michaelsen T.E., and Brekke O.H. Direct isolation of recombinant human antibodies against group B Neisseria meningitidis from scFv expression libraries. J. Immunol. Methods 283 (2003) 247
29. Thurmond L.M., Stimmel J.B., Ingram A.C., Ryan C.H., Murray D.M., Eberwein D.J., Witherspoon S.M., and Knick V.C. Adenocarcinoma cells exposed in vitro to Navelbine or Taxol increase Ep-CAM expression through a novel mechanism. Cancer Immunol. Immunother. 52 (2003) 429
30. In: Uhlén M., Hornes E., and Olsvik Ø. (Eds). Advances in Biomagnetic Separation (1994), BioTechniques Press, Westborough, MA
31. Walter G., Büssow K., Lueking A., and Glökler J. High-throughput protein arrays: prospects for molecular diagnosis. Trends Mol. Med. 8 (2002) 250
32. 2 autoantibody. Proc. Natl. Acad. Sci. U. S. A. 94 (1997) 1902
33. Wilson D.S., Keefe A.D., and Szostak J.W. The use of mRNA display to select high-affinity protein-binding peptides. Proc. Natl. Acad. Sci. U. S. A. 98 (2001) 3750
34. Winter, G.P., Ward, E.S., Güssow, D., 1990. Single domain ligands, receptors comprising said ligands, methods for their production, and use of said ligands and receptors. WO 90/05144.
35. Wittrup K.D. Protein engineering by cell-surface display. Curr. Opin. Biotechnol. 12 (2001) 395
36. Yeung Y.A., and Wittrup K.D. Quantitative screening of yeast surface-displayed polypeptide libraries by magnetic bead capture. Biotechnol. Prog. 18 (2002) 212