Novel strategies to overcome expression problems encountered with toxic proteins: Application to the production of Lac repressor proteins for NMR studies

Protein Expression and Purification

Volumn 67, Issue 2, 2009, Pages 104-112

  Romanuka, Julija ^a   Bulke, Heidi van den ^a   Kaptein, Robert ^a   Boelens, Rolf ^a   Folkers, Gert E ^a  

^a UTRECHT UNIVERSITY   (Netherlands)

Author keywords

Buffer conditions optimization; Isotope labeling; Lac repressor; NMR; Toxic proteins

Indexed keywords

AMIDE; BACTERIAL PROTEIN; HYBRID PROTEIN; ISOPROPYL THIOGALACTOSIDE; LACI PROTEIN, BACTERIA; REPRESSOR PROTEIN; SODIUM CHLORIDE;

AFFINITY CHROMATOGRAPHY; ARTICLE; BIOSYNTHESIS; CHEMISTRY; CULTURE TECHNIQUE; ESCHERICHIA COLI; GENETICS; ISOLATION AND PURIFICATION; ISOTOPE LABELING; METABOLISM; METHODOLOGY; MOLECULAR CLONING; NUCLEAR MAGNETIC RESONANCE; PH; TEMPERATURE;

AMIDES; BACTERIAL PROTEINS; CELL CULTURE TECHNIQUES; CHROMATOGRAPHY, AFFINITY; CLONING, MOLECULAR; ESCHERICHIA COLI; HYDROGEN-ION CONCENTRATION; ISOPROPYL THIOGALACTOSIDE; ISOTOPE LABELING; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; RECOMBINANT FUSION PROTEINS; REPRESSOR PROTEINS; SODIUM CHLORIDE; TEMPERATURE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 67650147985     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2009.05.008     Document Type: Article

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