Expression in Escherichia coli of cytoplasmic portions of the cystic fibrosis transmembrane conductance regulator: Apparent bacterial toxicity of peptides containing R-domain sequences

Protein Expression and Purification

Volumn 7, Issue 1, 1996, Pages 45-50

  Yike, Iwona ^a   Zhang, Yan ^a   Ye, Jin ^a   Dearborn, Dorr G ^a,b  

^a CASE WESTERN RESERVE UNIVERSITY   (United States)

^b CASE WESTERN RESERVE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0030087558     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.1996.0007     Document Type: Article

References (19)

1. 1. Collins, F. S. (1992) Cystic fibrosis: Molecular biology and therapeutic implications. Science 256, 774-779.
2. 2. Chang, X.-B., Hou, Y.-H., Jensen, T. J., and Riordan, J. R. (1994) Mapping of cystic fibrosis transmembrane conductance regulator membrane topology by glycosylation site insertion. J. Biol. Chem. 269, 18572-18575.
3. 3. Tsui, L.-C. (1992) The spectrum of cystic fibrosis mutations. Trends Genet. 8, 392-398.
4. 4. Bear, C. E., Li, C., Kartner, N., Bridges, R. J., Jensen, T. J., Ramjeesingh, M., and Riordan, J. R. (1992) Purification and functional reconstitution of the cystic fibrosis transmembrane conductance regulator (CFTR). Cell 68, 809-818.
5. 5. Riordan, J. R., Rommens, J. M., Kerem, B. S., Alom, N., Rozmahel, R., Grzelczak, Z., Zielenski, J., Lok, S., Plavsic, N., Chou, J.-L., Drumm, M. L., Iannuzzi, M. C., Collins, F. S., and Tsui, L.-C. (1989) Identification of the cystic fibrosis gene. Cloning and characterization of complementary DNA. Science 245, 1066-1073.
6. 6. Gregory, R. J., Cheng, S. H., Rich, D. P., Marshall, J., Paul, S., Hehir, K., Ostedgaard, L., Klinger, K., Welsh, M., and Smith, A. E. (1990) Expression and characterization of the cystic fibrosis transmembrane conductance regulator. Nature 347, 382-386.
7. 7. Yike, I., Ye, J., Zhang, Y., Manavalan, P., Gerken, T. A., and Dearborn, D. G. The first nucleotide binding fold of the cystic fibrosis transmembrane conductance regulator: Production and characterization of wild-type and ΔF508 mutant forms of a recombinant peptide model. Protein Sci., in press.
8. 8. Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989) "Molecular Cloning: A Laboratory Manual," Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
9. 4. Nature 227, 680-685.
10. 10. Studier, F. W., Rosenberg, A. H., Dunn, J. J., and Dubendorff, J. W. (1990) Use of T7 RNA polymerase to direct the expression of cloned genes. Methods Enzymol. 185, 62-89.
11. 11. Sharp, P. M., and Li, W. H. (1987) The codon adaptation index -a measure of directional synonymous codon usage bias and its potential application. Nucleic Acids Res. 15, 1281-1294.
12. 12. Rosenberg, A. H., Goldman, E., Dunn, J. J., Studier, F. W., and Zubay, G. (1993) Effects of consecutive AGG codons on translation in E. coli, demonstrated with a versatile codon test system. J. Bacteriol. 175, 716-722.
13. 13. Grodberg, J., and Dunn, J. J. (1988) ompT encodes the E. coli outer membrane protease that cleaves T7 RNA polymerase during purification. J. Bacteriol. 170, 1245-1253.
14. 14. Studier, F. W., and Moffatt, B. A. (1986) Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J. Mol. Biol. 189, 113-131.
15. 15. Hyde, S. C., Emsley, P., Hartshorn, M. J., Mimmack, M. M., Gileadi, U., Pearce, S. R., Gallagher, M. P., Gill, D. R., Hubbard, R. E., and Higgins, C. F. (1990) Structural model of ATP-binding proteins associated with cystic fibrosis, multidrug resistance and bacterial transport. Nature 346, 362-365.
16. 16. Gerchman, S. E., Graziano, V., and Ramakrishnan, V. (1994) Expression of chicken linker histones in E. coli: Sources of problems and methods for overcoming some of the difficulties. Protein Expression Purif. 5, 242-251.
17. 17. Rich, D. P., Gregory, R. J., Anderson, M. P., Manalavan, P., Smith, A. E., and Welsh, M. J. (1991) Effect of deleting the R domain on CFTR-generated chloride channels. Science 253, 205-207.
18. 18. Dulhanty, A. M., and Riordan, J. R. (1994) A two-domain model for the R domain of the cystic fibrosis transmembrane conductance regulator based on sequence similarities. FEBS Lett. 343, 109-114.
19. 19. Dulhanty, A. M., and Riordan, J. R. (1994) Phosphorylation by cAMP-dependent protein kinase causes a conformational change in the R domain of the cystic fibrosis transmembrane conductance regulator. Biochemistry 33, 4072-4079.