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note
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Because the parent compound (9b) of these analogues showed non-selective activity for B and T cells, we just performed lymphoproliferation assay on T-lymphocyte-activated cells as a general thing.
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note
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50 value was expressed as greater than the highest exposure concentration. Each dose response curve is composed of a vehicle (DMSO) group and seven treatment groups spanning an exposure range of 0.1-300 μM. Each value in the curve represents the mean of 4-8 wells in a plate. The α-GST and MTT assays were run from one plate as were the GSH/8-isoprostane assays, while the cell proliferation and ATP assays were run from separate plates.
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