Serum-free production and purification of chimeric IgA antibodies

Journal of Immunological Methods

Volumn 346, Issue 1-2, 2009, Pages 26-37

  Beyer, T ^a   Lohse, S ^a   Berger, S ^a   Peipp, M ^a   Valerius, T ^a   Dechant, M ^a  

^a UNIVERSITY OF KIEL   (Germany)

Author keywords

Antibody; IgA; Production; Purification

Indexed keywords

CHIMERIC ANTIBODY; GLUTAMATE AMMONIA LIGASE; RECOMBINANT ANTIBODY; RECOMBINANT IMMUNOGLOBULIN A1 ANTIBODY; RECOMBINANT IMMUNOGLOBULIN A2 ANTIBODY; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; ANIMAL CELL; ANTIBODY DEPENDENT CELLULAR CYTOTOXICITY; ANTIBODY PRODUCTION; ANTIBODY SPECIFICITY; ARTICLE; CANCER CELL CULTURE; CHO CELL; CONTROLLED STUDY; DRUG PURIFICATION; DRUG SYNTHESIS; EXPRESSION VECTOR; GEL PERMEATION CHROMATOGRAPHY; HUMAN; HUMAN CELL; LARGE SCALE PRODUCTION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; SCALE UP; SQUAMOUS CELL CARCINOMA;

ANIMALS; ANTIBODY SPECIFICITY; ANTIBODY-DEPENDENT CELL CYTOTOXICITY; BINDING SITES, ANTIBODY; CELL LINE, TUMOR; CELL PROLIFERATION; CHO CELLS; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, GEL; CRICETINAE; CRICETULUS; HUMANS; HYBRIDOMAS; IMMUNOGLOBULIN A; IMMUNOGLOBULIN HEAVY CHAINS; IMMUNOGLOBULIN LIGHT CHAINS; LEUKOCYTES, MONONUCLEAR; RECEPTOR, EPIDERMAL GROWTH FACTOR; RECOMBINANT FUSION PROTEINS; TRANSFECTION;

EID: 67449132105     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2009.05.002     Document Type: Article

References (43)

1. Areschoug T., Stalhammar-Carlemalm M., Karlsson I., and Lindahl G. Streptococcal beta protein has separate binding sites for human factor H and IgA-Fc. J. Biol. Chem. 277 (2002) 12642
2. Bebbington C.R., Renner G., Thomson S., King D., Abrams D., and Yarranton G.T. High-level expression of a recombinant antibody from myeloma cells using a glutamine synthetase gene as an amplifiable selectable marker. Biotechnology (N Y) 10 (1992) 169
3. Belew M., Juntti N., Larsson A., and Porath J. A one-step purification method for monoclonal antibodies based on salt-promoted adsorption chromatography on a 'thiophilic' adsorbent. J. Immunol. Methods 102 (1987) 173
4. Boel E., Verlaan S., Poppelier M.J., Westerdaal N.A., Van Strijp J.A., and Logtenberg T. Functional human monoclonal antibodies of all isotypes constructed from phage display library-derived single-chain Fv antibody fragments. J. Immunol. Methods 239 (2000) 153
5. Brezinsky S.C., Chiang G.G., Szilvasi A., Mohan S., Shapiro R.I., MacLean A., Sisk W., and Thill G. A simple method for enriching populations of transfected CHO cells for cells of higher specific productivity. J. Immunol. Methods 277 (2003) 141
6. Browne S.M., and Al-Rubeai M. Selection methods for high-producing mammalian cell lines. Trends Biotechnol. 25 (2007) 425
7. Carayannopoulos L., Hexham J.M., and Capra J.D. Localization of the binding site for the monocyte immunoglobulin (Ig) A-Fc receptor (CD89) to the domain boundary between Cα2 and Cα3 in human IgA1. J. Exp. Med. 183 (1996) 1579
8. Chintalacharuvu K.R., Gurbaxani B., and Morrison S.L. Incomplete assembly of IgA2 m(2) in Chinese hamster ovary cells. Mol. Immunol. 44 (2007) 3445
9. Chu L., and Robinson D.K. Industrial choices for protein production by large-scale cell culture. Curr. Opin. Biotechnol. 12 (2001) 180
10. Corthesy B. Recombinant immunoglobulin A: powerful tools for fundamental and applied research. Trends Biotechnol. 20 (2002) 65
11. de la Cruz Edmonds M.C., Tellers M., Chan C., Salmon P., Robinson D.K., and Markusen J. Development of transfection and high-producer screening protocols for the CHOK1SV cell system. Mol. Biotechnol. 34 (2006) 179
12. Dechant M., and Valerius T. IgA antibodies for cancer therapy. Crit. Rev. Oncol./ Hematol. 39 (2001) 69
13. Dechant M., Vidarsson G., Stockmeyer B., Repp R., Glennie M.J., Gramatzki M., van de Winkel J.G.J., and Valerius T. Chimeric IgA antibodies against HLA class II effectively trigger lymphoma cell killing. Blood 100 (2002) 4574
14. Dechant M., Beyer T., Schneider-Merck T., Weisner W., Peipp M., van de Winkel J.G., and Valerius T. Effector mechanisms of recombinant IgA antibodies against epidermal growth factor receptor. J. Immunol. 179 (2007) 2936
15. Falkenberg F.W., Weichert H., Krane M., Bartels I., Palme M., Nagels H.O., and Fiebig H. In vitro production of monoclonal antibodies in high concentration in a new and easy to handle modular minifermenter. J. Immunol. Methods 179 (1995) 13
16. Gregory R.L., Rundegren J., and Arnold R.R. Separation of human IgA1 and IgA2 using jacalin-agarose chromatography. J. Immunol. Methods 99 (1987) 101
17. Huls G., Heijnen I.A., Cuomo E., van der Linden J., Boel E., van de Winkel J.G., and Logtenberg T. Antitumor immune effector mechanisms recruited by phage display-derived fully human IgG1 and IgA1 monoclonal antibodies. Cancer Res. 59 (1999) 5778
18. Johansen F.E., Braathen R., and Brandtzaeg P. Role of J chain in secretory immunoglobulin formation. Scand. J. Immunol. 52 (2000) 240
19. Leibl H., Tomasits R., and Mannhalter J.W. Isolation of human serum IgA using thiophilic adsorption chromatography. Protein Expr. Purif. 6 (1995) 408
20. Lindahl G., Akerstrom B., Vaerman J.P., and Stenberg L. Characterization of an IgA receptor from group B streptococci: specificity for serum IgA. Eur. J. Immunol. 20 (1990) 2241
21. Mattu T.S., Pleass R.J., Willis A.C., Kilian M., Wormald M.R., Lellouch A.C., Rudd P.M., Woof J.M., and Dwek R.A. The glycosylation and structure of human serum IgA1, Fab, and Fc regions and the role of N-glycosylation on Fcα-receptor interactions. J. Biol. Chem. 273 (1998) 2260
22. Monteiro R.C., and van de Winkel J.G.J. IgA Fc receptors. Annu. Rev. Immunol. 21 (2003) 177
23. Morton H.C., Atkin J.D., Owens R.J., and Woof J.M. Purification and characterization of chimeric human IgA1 and IgA2 expressed in COS and Chinese hamster ovary cells. J. Immunol. 151 (1993) 4743
24. Nilson B.H., Solomon A., Bjorck L., and Akerstrom B. Protein L from Peptostreptococcus magnus binds to the kappa light chain variable domain. J. Biol. Chem. 267 (1992) 2234
25. Novak J., Julian B.A., Tomana M., and Mestecky J. IgA glycosylation and IgA immune complexes in the pathogenesis of IgA nephropathy. Semin. Nephrol. 28 (2008) 78
26. Otten M.A., Rudolph E., Dechant M., Tuk C.W., Reijmers R.M., Beelen R.H., van de Winkel J.G., and van Egmond M. Immature neutrophils mediate tumor cell killing via IgA but not IgG Fc receptors. J. Immunol. 174 (2005) 5472
27. Peipp M., Schneider-Merck T., Dechant M., Beyer T., van Bueren J.J., Bleeker W.K., Parren P.W., van de Winkel J.G., and Valerius T. Tumor cell killing mechanisms of epidermal growth factor receptor (EGFR) antibodies are not affected by lung cancer-associated EGFR kinase mutations. J. Immunol. 180 (2008) 4338
28. Perosa F., Carbone R., Ferrone S., and Dammacco F. Purification of human immunoglobulins by sequential precipitation with caprylic acid and ammonium sulphate. J. Immunol. Methods 128 (1990) 9
29. Raju T.S. Terminal sugars of Fc glycans influence antibody effector functions of IgGs. Curr. Opin. Immunol. 20 (2008) 471
30. Rifai A., Fadden K., Morrison S.L., and Chintalacharuvu K.R. The N-glycans determine the differential blood clearance and hepatic uptake of human immunoglobulin (Ig)A1 and IgA2 isotypes. J. Exp. Med. 191 (2000) 2171
31. Ronnmark J., Gronlund H., Uhlen M., and Nygren P.A. Human immunoglobulin A (IgA)-specific ligands from combinatorial engineering of protein A. Eur. J. Biochem. 269 (2002) 2647
32. Sandin C., Linse S., Areschoug T., Woof J.M., Reinholdt J., and Lindahl G. Isolation and detection of human IgA using a streptococcal IgA-binding peptide. J. Immunol. 169 (2002) 1357
33. Sinacore M.S., Drapeau D., and Adamson S.R. Adaptation of mammalian cells to growth in serum-free media. Mol. Biotechnol. 15 (2000) 249
34. Sondermann P., Huber R., Oosthuizen V., and Jacob U. The 3.2-A crystal structure of the human IgG1 Fc fragment-Fc gammaRIII complex. Nature 406 (2000) 267
35. Stenberg L., O Toole P.W., Mestecky J., and Lindahl G. Molecular characterization of protein Sir, a streptococcal cell surface protein that binds both immunoglobulin A and immunoglobulin G. J. Biol. Chem. 269 (1994) 13458
36. Thern A., Stenberg L., Dahlback B., and Lindahl G. Ig-binding surface proteins of Streptococcus pyogenes also bind human C4b-binding protein (C4BP), a regulatory component of the complement system. J. Immunol. 154 (1995) 375
37. van Egmond M., Damen C.A., van Spriel A.B., Vidarsson G., van Garderen E., and van de Winkel J.G. IgA and the IgA Fc receptor. Trends Immunol. 22 (2001) 205
38. Vidarsson G., van de Winkel J.G., and van Dijk M.A. Multiplex screening for functionally rearranged immunoglobulin variable regions reveals expression of hybridoma-specific aberrant V-genes. J. Immunol. Methods 249 (2001) 245
39. Wines B.D., and Hogarth P.M. IgA receptors in health and disease. Tissue Antigens 68 (2006) 103
40. Woof J.M., and Mestecky J. Mucosal immunoglobulins. Immunol. Rev. 206 (2005) 64-82
41. Woof J.M., and Kerr M.A. The function of immunoglobulin A in immunity. J. Pathol. 208 (2006) 270
42. Wurm F.M. Production of recombinant protein therapeutics in cultivated mammalian cells. Nat. Biotechnol. 22 (2004) 1393
43. Yoo E.M., and Morrison S.L. IgA: an immune glycoprotein. Clin. Immunol. 116 (2005) 3