The co-operative effect of physical and covalent protein adsorption on heterofunctional supports

Process Biochemistry

Volumn 44, Issue 7, 2009, Pages 757-763

  Bolivar, Juan M ^a   Mateo, Cesar ^a   Godoy, Cesar ^a   Pessela, Benevides C C ^b   Rodrigues, Dasciana S ^c   Giordano, Raquel L C ^c   Fernandez Lafuente, Roberto ^a   Guisan, Jose M ^a  

^a INSTITUTO DE CATÁLISIS Y PETROLEOQUÍMICA   (Spain)

^b INSTITUTO DE FERMENTACIONES INDUSTRIALES CSIC   (Spain)

^c FEDERAL UNIVERSITY OF SÃO CARLOS   (Brazil)

Author keywords

Amino epoxy supports; Immobilization of proteins on epoxy supports; Intermolecular covalent attachment; Intramolecular covalent attachment; Oriented immobilization; Two step immobilization

Indexed keywords

AMINO-EPOXY SUPPORTS; IMMOBILIZATION OF PROTEINS ON EPOXY SUPPORTS; INTERMOLECULAR COVALENT ATTACHMENT; INTRAMOLECULAR COVALENT ATTACHMENT; ORIENTED IMMOBILIZATION; TWO STEP IMMOBILIZATION;

ADSORPTION; AMINATION; AMINES; BACTERIOLOGY; CATALYSTS; DIFFERENTIAL AMPLIFIERS; DRUG PRODUCTS; ESCHERICHIA COLI; ION EXCHANGE; IONIC STRENGTH; PROGRAMMED CONTROL SYSTEMS;

ENZYMES;

ESCHERICHIA COLI; GEOBACILLUS THERMOCATENULATUS; THERMUS THERMOPHILUS;

EID: 67349248070     PISSN: 13595113     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.procbio.2009.03.012     Document Type: Article

References (43)

1. Cao L., van Langen l., and Scheldon R.A. Immobilised enzymes: carrier bound or carrier free?. Curr Opin Biotechnol 14 (2003) 387-394
2. Katchalski-Katzir E. Immobilized enzymes-learning from past successes and failures. Trends Biotechnol 11 (1993) 471-478
3. Rusmini F., Zhong Z., and Feijen J. Protein immobilization strategies for protein biochips. Biomacromolecules 8 (2007) 1775-1789
4. Sheldon R.A. Enzyme immobilization: the quest of optimal performance. Adv Synth Catal 349 (2007) 1289-1307
5. Wong C.H., and Whitesides G.M. Enzymes in synthetic organic chemistry (1994), Pergamon Press Elsevier Science Ltd., Oxford, UK
6. Turkova J. Oriented immobilization of biologically active proteins as a tool for revealing protein interactions and function. J Chromatogr B: Biomed Sci Appl 722 (1999) 11-31
7. Mateo C., Palomo J.M., Fernandez-Lorente G., Guisan J.M., and Fernandez-Lafuente R. Improvement of enzyme activity, stability and selectivity via immobilization techniques. Enzyme Microb Technol 40 (2007) 1451-1463
8. Pessela B.B.C., Fuentes M., Vian A., Garcia J.L., Carrascosa A.V., Mateo C., Guisan J.M., and Fernández-Lafuente R. The immobilization of a thermophilic beta-galactosidase on Sepabeads supports promotes the reduction of product inhibition. Full enzymatic hydrolysis of lactose in dairy products. Enzyme Microb Technol 33 (2003) 199-205
9. Palomo J.M., Fernandez-Lorente G., Mateo C., Fuentes M., Guisan J.M., and Fernandez-Lafuente R. Modulation of Mucor miehei lipase properties via directed immobilization on different heterofunctional epoxy resins. Hydrolytic resolution of (R,S)-2-phenyl-2-butyroylacteic acid. J Mol Catal B: Enzyme 21 (2003) 201-210
10. Mansfeld J., Vriend G., Van den Burg B., Eijsink V.G., and Ulbrich-Hofmann R. Probing the unfolding region in a thermolysin-like protease by site-specific immobilization. Biochemistry 38 (1999) 8240-8245
11. Mansfeld J., and Ulbrich-Hofmann R. Site-specific and random immobilization of thermolysin-like proteases reflected in the thermal inactivation kinetics. Biotechnol Appl Biochem 32 (2000) 189-195
12. Gianfreda L., and Scarfi M.R. Enzyme stabilization: state of the art. Mol Cell Biochem 100 (1991) 97-128
13. Gupta M.N. Thermostabilization of proteins. Biotechnol Appl Biochem 14 (1991) 1-11
14. Pedroche J., Mateo C., Girón-Calle J., Alaiz M., Vioque J., Guisán J.M., and Millán F. Effect of the support and experimental conditions in the intensity of the multipoint covalent attachment of proteins on glyoxyl-agarose supports. Correlation between enzyme-support linkages and thermal stability. Enzyme Microb Technol 40 (2007) 1160-1166
15. Mateo C., Grazú V., Pessela B.C.C., Montes T., Palomo J.M., Torres R., López-Gallego F., Fernández-Lafuente R., and Guisán J.M. Advances in the design of new epoxy supports for enzyme immobilization-stabilization. Biochem Soc Trans 35 (2007) 1593-1601
16. Boller T., Meier C., and Menzler S. EUPERGIT oxirane acrylic beads: how to make enzymes fit for biocatalysis. Org Process Res Dev 6 (2002) 509-519
17. Katchalski-Katzir E., Kraemer D.M., and Eupergit C. A carrier for immobilization of enzymes of industrial potential. J Mol Catal B: Enzyme 10 (2000) 157-176
18. Wheatley J.B., and Schmidt D.E. Salt induced immobilization of affinity ligands onto epoxide-activated supports. J Chromatogr A 849 (1999) 1-12
19. Wheatley J.B., and Schmidt D.E. Salt induced immobilization of proteins on a high-performance liquid chromatographic epoxide affinity support. J Chromatogr A 644 (1993) 11-16
20. Wheatley J.B., Lyttle M.H., Hocker M.D., and Schmidt Jr. D.E. Salt-induced immobilizations of DNA oligonucleotides on an epoxide-activated high-performance liquid chromatographic affinity support. J Chromatogr A 726 (1996) 77-90
21. Bauer-Arnaz K., Napolitano E.W., Roberts D.N., Montali J.A., Hughes B.R., and Schmidt Jr. D.E. Salt-induced immobilization of small affinity ligands on an epoxide activated affinity support. J Chromatogr A 803 (1998) 73-82
22. Turkova J., Blaha K., and Malanikova M. Methacrylate gels with epoxide groups as supports for immobilization of enzymes in pH range 3-12. Biochim Biophys Acta (BBA)-Enzymol 524 (1978) 162-169
23. Mateo C., Fernández-Lorente G., Abian O., Fernández-Lafuente R., and Guisán J.M. Multifunctional epoxy-supports: a new tool to improve the covalent immobilization of proteins. The promotion of physical adsorptions of proteins on the supports before their covalent linkage. Biomacromolecules 1 (2000) 739-745
24. Fuentes M., Batalla P., Grazu V., Pessela B.C.C., Mateo C., Montes T., Hermoso J.A., Guisan J.M., and Fernandez-Lafuente R. Mixed ion exchangers supports as useful ion exchangers for protein purification. Purification of penicillin G acylase from E. coli. Biomacromolecules 8 (2007) 703-707
25. Pessela B.C.C., Betancor L., Munilla R., Fuentes M., Carrascosa A.V., Vian A., Fernandez-Lafuente R., and Guisan J.M. Ion exchange using poorly activated supports, an easy way for purifying of large proteins. J Chromatogr A 1034 (2004) 155-159
26. Pessela B.C., Fuentes M., Mateo C., Munilla R., Carrascosa A.V., Fernandez-Lafuente R., and Guisan J.M. Purification and very strong reversible immobilization of large proteins on anionic exchangers by controlling the support and the immobilization conditions. Enzyme Microb Technol 39 (2006) 909-915
27. Sio C.F., Quax W.J., and Improved. β-Lactam acylases and their use as industrial biocatalysts. Curr Opin Biotechnol 15 (2004) 349-355
28. Arroyo M., De La Mata I., Acebal C., and Castillón M.P. Biotechnological applications of penicillin acylases: state-of-the-art. Appl Microbiol Biotechnol 60 (2003) 507-514
29. Schmidt-Dannert C., Rua M.L., Wahl S., and Schmid R.D. Bacillus thermocatenulatus lipase: a thermoalkalophilic lipase with interesting properties. Biochem Soc Trans 25 (1997) 178-182
30. Martín J.R., Nus M., Gago J.V.S., and Sánchez-Montero J.M. Selective esterification of phthalic acids in two ionic liquids at high temperatures using a thermostable lipase of Bacillus thermocatenulatus: a comparative study. J Mol Catal B: Enzyme 52-53 (2008) 162-167
31. Bolivar J.M., Mateo C., Rocha-Martin J., Cava F., Berenguer J., Fernandez-Lafuente R., and Guisan J.M. The adsorption of multimeric enzymes on very lowly activated supports involves more enzyme subunits: stabilization of a glutamate dehydrogenase from Thermus thermophilus by immobilization on heterofunctional supports. Enzyme Microb Technol 44 (2009) 139-144
32. Van der Donk W., and Zhao H. Recent developments in pyridine nucleotide regeneration. Curr Opin Biotechnol 14 (2003) 421-426
33. Rodriguez B.B., Bolbot J.A., and Tothill I.E. Development of urease and glutamic dehydrogenase amperometric assay for heavy metals screening in polluted samples. Biosens Bioelectron 19 (2004) 1157-1167
34. Palomo J.M., Segura R.L., Fernandez-Lorente G., Pernas M., Rua M.L., Guisan J.M., and Fernandez-Lafuente R. Purification, immobilization, and stabilization of a lipase from Bacillus thermocatenulatus by interfacial adsorption on hydrophobic supports. Biotechnol Prog 20 (2004) 630-635
35. Pessela B.C.C., Vian A., Mateo C., Fernánndez-Lafuente R., García J.L., Guisán J.M., and Carrascosa A.V. Overproduction of Thermus sp. strain T2 β-galactosidase in Escherichia coli and preparation by using tailor-made metal chelate supports. Appl Environ Microbiol 69 (2003) 1967-1972
36. Bolivar J.M., Cava F., Mateo C., Rocha-Martín J., Guisán J.M., Berenguer J., and Fernández-Lafuente R. Immobilization-stabilization of a new recombinant glutamate dehydrogenase from Thermus thermophilus. Appl Microb Biotechnol 80 (2008) 49-58
37. Bradford M.M. A rapid and sensitive method for the quantization of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem 72 (1976) 248-254
38. Armisén P., Mateo C., Cortés E., Barredo J.L., Salto F., Diez B., Rodés L., García J.L., Fernández-Lafuente R., and Guisán J.M. Selective adsorption of poly-His tagged glutaryl acylase on tailor-made metal chelate supports. J Chromatogr A 848 (1999) 61-70
39. Grazú V., Abian O., Mateo C., Batista-Viera F., Fernández-Lafuente R., and Guisán J.M. Novel bifunctional epoxy/thiol-reactive support to immobilize thiol containing proteins by the epoxy chemistry. Biomacromolecules 4 (2003) 1495-1501
40. Habeeb A.F.S.A. Determination of free amino groups in proteins by trinitrobenzenesulfonicacid. Anal Biochem 14 (1966) 328-336
41. Snyder S.L., and Sobocinski P.Z. An improved 2,4,6-trinitrobenzenesulfonic acid method for the determination of amines. Anal Biochem 64 (1975) 284-288
42. Batalla P., Fuentes M., Mateo C., Grazu V., Fernandez-Lafuente R., and Guisan J.M. Covalent immobilization of antibodies on finally inert support surfaces through their surface regions having the highest densities in carboxyl groups. Biomacromolecules 9 (2008) 2230-2236
43. Fernandez-Lafuente R., Rosell C.M., Rodriguez V., Santana C., Soler G., Bastida A., and Guisan J.M. Preparation of activated supports containing low pK amino groups. A new tool for protein immobilization via the carboxyl coupling method. Enzyme Microb Technol 15 (1993) 546-550