Marker-disruptive gene integration and URA3 recycling for multiple gene manipulation in Saccharomyces cerevisiae

Applied Microbiology and Biotechnology

Volumn 83, Issue 4, 2009, Pages 783-789

  Kaneko, Shohei ^a   Tanaka, Tsutomu ^b   Noda, Hideo ^a   Fukuda, Hideki ^b   Akada, Rinji ^c   Kondo, Akihiko ^b  

^a Research and Development Laboratory   (Japan)

^b KOBE UNIVERSITY   (Japan)

^c YAMAGUCHI UNIVERSITY   (Japan)

Author keywords

Glucosidase; Ethanol fermentation; Gene integration; Marker recycling

Indexed keywords

BASE PAIRS; CELL SURFACES; CELLOBIOSE; ETHANOL FERMENTATION; GENETIC MANIPULATIONS; GLUCOSIDASE; MARKER GENES; MARKER RECYCLING; MULTIPLE GENES; REPEATED SEQUENCES; SACCHAROMYCES CEREVISIAE; SHORT SEQUENCES; TRANSFORMANTS;

BIOCHEMICAL ENGINEERING; CELL MEMBRANES; ETHANOL; FERMENTATION; GENES; INDUSTRIAL APPLICATIONS; RECYCLING; YEAST;

INTEGRATION;

ALCOHOL; BETA GLUCOSIDASE; CELLOBIOSE;

CHROMOSOME; ENZYME ACTIVITY; ETHANOL; FERMENTATION; GENE EXPRESSION; GENETIC ENGINEERING; HOMOLOGY; YEAST;

ARTICLE; FUNGAL GENE; FUNGAL GENETICS; GENE DELETION; GENE EXPRESSION; GENE LOCUS; GENE SEQUENCE; GENETIC MANIPULATION; MARKER GENE; NONHUMAN; POLYMERASE CHAIN REACTION; SACCHAROMYCES CEREVISIAE; SEQUENCE HOMOLOGY; URA3 GENE;

BETA-GLUCOSIDASE; CULTURE MEDIA; FUNGAL PROTEINS; MUTAGENESIS, INSERTIONAL; OROTIC ACID; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SELECTION (GENETICS);

SACCHAROMYCES CEREVISIAE;

EID: 67349137115     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-009-2038-0     Document Type: Article

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