Mis-targeting of multiple gene disruption constructs containing hisG

Current Genetics

Volumn 38, Issue 4, 2000, Pages 188-190

  Davidson, J F ^a   Schiestl, R H ^a  

^a HARVARD SCHOOL OF PUBLIC HEALTH   (United States)

Author keywords

Gene targeting; HisG; Homology; Saccharomyces

Indexed keywords

FUNGAL DNA; HISTIDINE;

ARTICLE; BASE PAIRING; CONTROLLED STUDY; FUNGAL GENETICS; GENE CASSETTE; GENE CONSTRUCT; GENE DISRUPTION; GENE INSERTION; GENE LOCUS; GENE SEQUENCE; GENE TARGETING; GENETIC MARKER; GENETIC RECOMBINATION; GENETIC TRANSCRIPTION; GENETIC TRANSFORMATION; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE; SEQUENCE HOMOLOGY;

SACCHAROMYCES CEREVISIAE;

EID: 0033739244     PISSN: 01728083     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002940000154     Document Type: Article

References (9)

1. A method for gene disruption that allows repeated use of URA3 selection in the construction of multiply disrupted yeast strains
2. A positive selection for mutants lacking orotidine-5'-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance
3. Improved method for high efficiency transformation of intact yeast cells
4. Microhomology mediated PCR targeting in Saccharomyces cerevisiae
5. Designer deletion and prototrophic strains derived from Saccharomyces cerevisiae strain W303-1a
6. Integration of DNA fragments by illegitimate recombination in Saccharomyces cerevisiae
7. A 2-micron DNA-based marker recycling system for multiple gene disruption in the yeast Saccharomyces cerevisiae
8. The frequency of gene targeting in yeast depends on the number of target copies
9. Gene targeting in normal and amplified cell lines