Construction of a xylan-fermenting yeast strain through codisplay of xylanolytic enzymes on the surface of xylose-utilizing Saccharomyces cerevisiae cells

Applied and Environmental Microbiology

Volumn 70, Issue 9, 2004, Pages 5407-5414

  Katahira, Satoshi ^a   Fujita, Yasuya ^a   Mizuike, Atsuko ^a   Fukuda, Hideki ^a   Kondo, Akihiko ^a  

^a KOBE UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

BIOMASS; CARBOHYDRATES; CELLULOSE; CHEMICAL ANALYSIS; ENZYMES; ETHANOL; FERMENTATION; LIQUID CHROMATOGRAPHY; STRAIN MEASUREMENT;

CEREVISIAE; HEMICELLULOSE; LIGNECELLULOSE;

YEAST;

AGGLUTININ; ALCOHOL; ALPHA AGGLUTININ; CARBOHYDRATE; CELL PROTEIN; OXIDOREDUCTASE; PROTEIN XYLA; UNCLASSIFIED DRUG; XYLAN; XYLAN 1,4 BETA XYLOSIDASE; XYLAN ENDO 1,3 BETA XYLOSIDASE; XYLITOL DEHYDROGENASE; XYLOBIOSE; XYLOSE; XYLOSE REDUCTASE; XYLOTRIOSE; XYLULOKINASE;

MICROBIOLOGY;

ARTICLE; BIOMASS; BIRCH; CELL SURFACE; ENZYME ANALYSIS; FERMENTATION TECHNIQUE; GLUCOSE UTILIZATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROLYSIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PICHIA STIPITIS; SACCHAROMYCES CEREVISIAE; STRAIN IDENTIFICATION; SURFACE PROPERTY; TRICHODERMA REESEI;

BASE SEQUENCE; DNA PRIMERS; ENDO-1,4-BETA XYLANASES; FERMENTATION; FUNGI; KINETICS; MOLECULAR SEQUENCE DATA; RESTRICTION MAPPING; SACCHAROMYCES CEREVISIAE; XYLANS; XYLOSE; YEASTS;

XYLANS; YEASTS;

ASPERGILLUS; ASPERGILLUS ORYZAE; HYPOCREA JECORINA; PICHIA; PICHIA STIPITIS; SACCHAROMYCES; SACCHAROMYCES CEREVISIAE; TRICHODERMA;

EID: 4644280289     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.70.9.5407-5414.2004     Document Type: Article

References (43)

1. Aristidou, A., and M. Penttilä. 2000. Metabolic engineering applications to renewable resource utilization. Curr. Opin. Biotechnol. 11:187-198.
2. Biely, P. 1985. Microbial xylanolytic systems. Trends Biotechnol. 3:286-290.
3. Crous, J. M., I. S. Pretorius, and W. H. Van Zyl. 1995. Cloning and expression of an Aspergillus kawachii endo-1,4-β-xylanase gene in Saccharomyces cerevisiae. Curr. Genet. 28:467-473.
4. Dekker, R. F. H. 1983. Bioconversion of hemicellulose: aspects of hemicellulose production by Trichodema reesei QM9414 and enzymatic saccharification of hemicellulose. Biotechnol. Bioeng. 25:1127-1146.
5. Den Haan, R., and W. H. Van Zyl. 2001. Differential expression of the Trichoderma reesei β-xylanase II (xyn2) gene in the xylose-fermenting yeast Pichia stipitis. Appl. Microbiol. Biotechnol. 57:521-527.
6. Den Haan, R., and W. H. Van Zyl. 2003. Enhanced xylan degradation and utilisation by Pichia stipitis overproducing fungal xylanolytic enzymes. Enzyme Microb. Technol. 33:620-628.
7. Desphande, V., A. Lachke, C. Mishra, S. Keskar, and M. Rao. 1986. Mode of action and properties of xylanase and β-xylosidase from Neurospora crassa. Biotechnol. Bioeng. 28:1832-1837.
8. De Vries, R. P., H. C. M. Rester, C. H. Poulsen, J. A. E. Benen, and J. Visser. 2000. Synergy between enzymes ùom Aspergillus involved in the degradation of plant cell wall polysaccharides. Carbohydr. Res. 327:401-410.
9. Dubois, M., K. A. Gilles, J. K. Hamilton, P. A. Reberse, and F. Smith. 1956. Colorimetric method for determination of sugars and related substances. Anal. Chem. 28:350-356.
10. Eliasson, A., C. Christensson, C. F. Wahlbom, and B. Hahn-Hägerdal. 2000. Anaerobic xylose fermentation by recombinant Saccharomyces cerevisiae carrying XYL1, XYL2, and XKS1 in mineral medium chemostat cultures. Appl. Environ. Microbiol. 66:3381-3386.
11. Fujita, Y., J. Ito, M. Ueda, H. Fukuda, and A. Kondo. 2004. Synergistic saccharification, and direct fermentation to ethanol, of amorphous cellulose by use of an engineered yeast strain codisplaying three types of cellulolytic enzyme. Appl. Environ. Microbiol. 70:1207-1212.
12. Fujita, Y., S. Katahira, M. Ueda, A. Tanaka, H. Okada, Y. Morikawa, H. Fukuda, and A. Kondo. 2002. Construction of whole-cell biocatalyst for xylan degradation through cell-surface xylanase display in Saccharomyces cerevisiae. J. Mol. Catal. B Enzym. 17:189-195.
13. Fujita, Y., S. Takahashi, M. Ueda, A. Tanaka, H. Okada, Y. Morikawa, T. Kawaguchi, M. Arai, H. Fukuda, and A. Kondo. 2002. Direct and efficient production of ethanol from cellulosic material with a yeast strain displaying cellulolytic enzymes. Appl. Environ. Microbiol. 68:5136-5141.
14. Herrmann, M. C., M. Vrsanska, M. Jurickova, J. Hirsch, P. Biely, and C. P. Kubicek. 1997. The β-D-xylosidase of Trichoderma reesei is a multifunctional β-D-xylan xylohydrolase. Biochem. J. 321:375-381.
15. Ho, N. W. Y., Z. Chen, and A. P. Brainard. 1998. Genetically engineered Saccharomyces yeast capable of effective cofermentation of glucose and xylose. Appl. Environ. Microbiol. 64:1852-1859.
16. Hrmova, M., P. Biely, and M. Vrsanska. 1989. Cellulose- and xylan-degrading enzymes of Aspergillus terreus and Aspergillus niger. Enzyme Microb. Technol. 11:610-616.
17. Jeffries, T. W. 1983. Utilization of xylose by bacteria, yeasts, and fungi. Adv. Biochem. Eng. 27:1-32.
18. Jeppsson, M., B. Johansson, B. Hahn-Hägerdal, and M. F. Gorwa-Grauslund. 2002. Reduced oxidative pentose phosphate pathway flux in recombinant xylose-utilizing Saccharomyces cerevisiae strains improves the ethanol yield from xylose. Appl. Environ. Microbiol. 68:1604-1609.
19. Johansson, B., C. Christensson, T. Hobley, and B. Hahn-Hägerdal. 2001. Xylulokinase overexpression in two strains of Saccharomyces cerevisiae also expressing xylose reductase and xylitol dehydrogenase and its effect on fermentation of xylose and lignocellulosic hydrolysate. Appl. Environ. Microbiol. 67:4249-4255.
20. Kanai, T., H. Atomi, K. Umemura, H. Ueno, Y. Teranishi, M. Ueda, and A. Tanaka. 1996. A novel heterologous gene expression system in Saccharomyces cerevisiae using the isocitrate lyase gene promoter from Candida tropicalis. Appl. Microbiol. Biotechnol. 44:759-765.
21. Kitamoto, N., S. Yoshino, K. Ohmiya, and N. Tsukagoshi. 1999. Sequence analysis, overexpression, and antisense inhibition of a β-xylosidase gene, xylA, from Aspergillus oryzae KBN616. Appl. Environ. Microbiol. 65:20-24.
22. Kondo, A., and M. Ueda. 2004. Yeast cell-surface display-applications of molecular display. Appl. Microbiol. Biotechnol. 64:28-40.
23. Kulkarni, N., A. Shendye, and M. Rao. 1999. Molecular and biotechnological aspects of xylanases. FEMS Microbiol. Rev. 23:411-456.
24. La Grange, D. C., I. S. Pretorius, M. Claeyssens, and W. H. Van Zyl. 2001. Degradation of xylan to D-xylose by recombinant Saccharomyces cerevisiae coexpressing the Aspergillus niger β-xylosidase (xlnD) and the Trichoderma reesei xylanase II (xyn2) genes. Appl. Environ. Microbiol. 67:5512-5519.
25. La Grange, D. C., I. S. Pretorius, and W. H. Van Zyl. 1996. Expression of a Trichoderma reesei β-xylanase gene (XYN2) in Saccharomyces cerevisiae. Appl. Environ. Microbiol. 62:1036-1044.
26. La Grange, D. C., I. S. Pretorius, and W. H. Van Zyl. 1997. Cloning of the Bacillus pumilus β-xylosidase gene (xynB) and its expression in Saccharomyces cerevisiae. Appl. Microbiol. Biotechnol. 47:262-266.
27. Li, X.-L., and L. G. Lijungdahl. 1996. Expression of Aureobasidium pullulans xynA in, and secretion of the xylanase from, Saccharomyces cerevisiae. Appl. Environ. Microbiol. 62:209-213.
28. Margolles-Clark, E., M. Tenkanen, T. Nakari-Setälä, and M. Penttilä. 1996. Cloning of genes encoding α-L-arabinofuranosidase and β-xylosidase from Trichoderma reesei by expression in Saccharomyces cerevisiae. Appl. Environ. Microbiol. 62:3840-3846.
29. Moreau, A., S. Durand, and R. Morosoli. 1992. Secretion of a Cryptococcus albidus xylanase in Saccharomyces cerevisiae. Gene 116:109-113.
30. Morosoli, R., E. Zalce, and S. Durand. 1993. Secretion of a Cryptococcus albidus xylanase in Pichia stipitis resulting in a xylan fermenting transformant. Curr. Genet. 24:94-99.
31. Park, Y. S., S. W. Kang, J. S. Lee, S. I. Hong, and S. W. Kim. 2002. Xylanase production in solid state fermentation by Aspergillus niger mutant using statistical experimental designs. Appl. Microbiol. Biotechnol. 58:761-766.
32. Pérez-González, J. A., L. H. De Grat, J. Visser, and D. Ramon. 1996. Molecular cloning and expression in Saccharomyces cerevisiae of two Aspergillus nidulans xylanase genes. Appl. Environ. Microbiol. 62:2179-2182.
33. Poutanen, K., and J. Puls. 1988. Characteristics of Trichoderma reesei β-xylosidase and its use in the hydrolysis of solubilized xylans. Appl. Microbiol. Biotechnol. 28:425-432.
34. Sawai-Hatanaka, H., T. Ashikari, Y. Tanaka, Y. Asada, T. Nakayama, H. Minakata, N. Kunishima, K. Fukuyama, H. Yamada, Y. Shibano, and T. Amachi. 1995. Cloning, sequencing, and heterologous expression of a gene coding for Arthromyces ramosus peroxidase. Biosci. Biotechnol. Biochem. 59:1221-1228.
35. Tajima, M., Y. Nogi, and T. Fukasawa. 1985. Primary structure of the Saccharomyces cerevisiae GAL7 gene. Yeast 1:67-77.
36. Takahashi, S., M. Ueda, and A. Tanaka. 2001. Function of the prosequence for in vivo folding and secretion of active Rhizopus oryzae lipase in Saccharomyces cerevisiae. Appl. Microbiol. Biotechnol. 55:454-462.
37. Teeri, T. T., A. Koivula, M. Linder, G. Wohlfahrt, C. Divne, and T. A. Jones. 1998. Trichoderma reesei cellobiohydrolases: why so efficient on crystalline cellulose? Biochem. Soc. Trans. 26:173-178.
38. Thomson, J. A. 1993. Molecular biology of xylan degradation. FEMS Microbiol. Rev. 10:65-82.
39. Törrönen, A., A. Harkki, and J. Routinen. 1994. Three-dimensional structure of endo-l,4-β-xylanase II from Trichoderma reesei: two conformational states in the active site. EMBO J. 13:2493-2501.
40. Törrönen, A., R. L. Mach, R. Messner, R. Gonzalez, N. Kalkkinen, A. Harkki, and C. P. Kubicek. 1992. The two major xylanases from Trichoderma reesei: characterization of both enzymes and genes. Bio/Technology 10:1461-1465.
41. Tsuchiya, K., S. Tada, K. Gomi, K. Kitamoto, C. Kumagai, and G. Tamura. 1992. Deletion analysis of the Taka-amylase A gene promoter using a homologous transformation system in Aspergillus oryzae. Biosci. Biotechnol. Biochem. 56:1849-1853.
42. Wong, K. K. Y., and J. N. Saddler. 1992. Trichoderma xylanases, their properties and application. Crit. Rev. Biotechnol. 12:413-435.
43. Wood, T. M., and K. M. Bhat. 1988. Methods for measuring cellulase activities. Methods Enzymol. 160:87-112.