pMPY-ZAP: A reusable polymerase chain reaction-directed gene disruption cassette for Saccharomyces cerevisiae

Yeast

Volumn 12, Issue 2, 1996, Pages 129-134

  Schneider, B L ^a   Steiner, B ^a   Seufert, W ^a,b   Futcher, A B ^a  

^a Watson School of Biological Sciences   (United States)

^b UNIVERSITY OF MUNICH   (Germany)

Author keywords

Gene disruption; PCR mediated disruption; Saccharomyces cerevisiae; Uraduction

Indexed keywords

ARTICLE; CONTROLLED STUDY; GENE DISRUPTION; GENETIC ANALYSIS; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE;

BASE SEQUENCE; GENETIC MARKERS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, INSERTIONAL; OROTIDINE-5'-PHOSPHATE DECARBOXYLASE; POLYMERASE CHAIN REACTION; SACCHAROMYCES CEREVISIAE; SELECTION (GENETICS); TRANSFORMATION, GENETIC;

SACCHAROMYCES CEREVISIAE;

EID: 0030051339     PISSN: 0749503X     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0061(199602)12:2<129::AID-YEA891>3.0.CO;2-O     Document Type: Article

References (11)

1. Alani, E., Cao, L. and Kleckner, N. (1987). A method for gene disruption that allows repeated use of URA3 selection in the construction of multiply disrupted yeast strains. Genetics 116, 541-545.
2. Ausubel, F. M., et al. (Eds) (1992). Current Protocols in Molecular Biology. Greene and Wiley, New York.
3. Baudin, A., Ozier-Kalogeropoulos, O., Denouel, A., Lacroute, F. and Culin, C. (1993). A simple and efficient method for direct gene deletion in Saccharomyces cerevisiae. Nucl. Acids Res. 21, 3329-3330.
4. Guthrie, C. and Fink, G. R. (1991). Guide to Yeast Genetics and Molecular Biology. Methods in Enzymology, vol. 194. Academic Press, New York.
5. Ko, C. H., Liang, H. and Gaber, R. F. (1993). Role of multiple glucose transporters in Saccharomyces cerevisiae. Mol. Cell. Biol. 13, 638-648.
6. Rothstein, R. (1991). Targeting, disruption, replacement, and allele rescue: integrative DNA transformation in yeast. In Guide to Yeast Genetics and Molecular Biology. Methods in Enzymology, vol. 194. Academic Press, New York, 281-301.
7. Sauer, B. (1994). Recycling selectable markers in yeast. Biotechniques 16, 1086-1088.
8. SIC1 controls the G1 to S transition in S. cerevisiae. Cell 79, 233-244.
9. Singer, M. S. and Gottschling, D. E. (1994). TLC1: Template RNA component of Saccharomyces cerevisiae telomerase. Science 266, 404-409.
10. Thomas, B. J. and Rothstein, R. (1989). Elevated recombination rates in transcriptionally active DNA. Cell 56, 619-630.
11. Vidai, M. and Gaber, R. F. (1994). Selectable marker replacement in Saccharomyces cerevisiae. Yeast 10, 141-149.