Parasites bearing a single copy of the multi-drug resistance gene (pfmdr-1) with wild-type SNPs predominate amongst Plasmodium falciparum isolates from Malawi

Acta Tropica

Volumn 111, Issue 1, 2009, Pages 78-81

  Nkhoma, Standwell ^a,b,c   Nair, Shalini ^c   Mukaka, Mavuto ^a   Molyneux, Malcolm E ^a,b   Ward, Stephen A ^b   Anderson, Timothy J C ^c  

^a UNIVERSITY OF MALAWI   (Malawi)

^b LIVERPOOL SCHOOL OF TROPICAL MEDICINE   (United Kingdom)

^c SOUTHWEST FOUNDATION FOR BIOMEDICAL RESEARCH   (United States)

Author keywords

Antimalarial resistance; Artemisinin combination therapy (ACT); Coartem; Copy number; Malawi; Multi drug resistance gene (pfmdr 1); Plasmodium falciparum; Single nucleotide polymorphisms (SNPs)

Indexed keywords

ARTEMETHER; ARTEMETHER PLUS BENFLUMETOL; BENFLUMETOL; DIHYDROARTEMISININ; MEFLOQUINE; MULTIDRUG RESISTANCE PROTEIN 1; PHENYLALANINE; TYROSINE;

DRUG RESISTANCE; HEALTH POLICY; INFECTIOUS DISEASE; PARASITE; POLYMORPHISM; PROTOZOAN;

AFRICA; ARTICLE; CHILD; CONTROLLED STUDY; DRUG SENSITIVITY; GENETIC SUSCEPTIBILITY; GENOTYPE; HUMAN; HUMAN CELL; IN VITRO STUDY; MALARIA FALCIPARUM; MALAWI; NONHUMAN; PARASITE; PARASITE ISOLATION; PLASMODIUM FALCIPARUM; PRESCHOOL CHILD; SENSITIVITY ANALYSIS; SEQUENCE ANALYSIS; SINGLE NUCLEOTIDE POLYMORPHISM; WILD TYPE;

AMINO ACID SUBSTITUTION; ANIMALS; ANTIMALARIALS; ARTEMISININS; CHILD, PRESCHOOL; DRUG COMBINATIONS; DRUG RESISTANCE, MULTIPLE; ETHANOLAMINES; FLUORENES; GENE DOSAGE; HUMANS; INFANT; INHIBITORY CONCENTRATION 50; MALARIA, FALCIPARUM; MALAWI; MULTIDRUG RESISTANCE-ASSOCIATED PROTEINS; MUTATION, MISSENSE; PARASITIC SENSITIVITY TESTS; PLASMODIUM FALCIPARUM; POLYMORPHISM, SINGLE NUCLEOTIDE;

PLASMODIUM FALCIPARUM;

EID: 67349090962     PISSN: 0001706X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.actatropica.2009.01.011     Document Type: Article

References (20)

1. Anderson T.J., Nair S., Qiu W.G., Singlam S., Brockman A., Paiphun L., and Nosten F. Are transporter genes other than the chloroquine resistance locus (pfcrt) and multi drug resistance gene (pfmdr) associated with antimalarial drug resistance?. Antimicrob. Agents Chemother. 272 (2005) 1153-1161
2. Dokomajilar C., Nsobya S.L., Greenhouse B., Rosenthal P.J., and Dorsey G. Selection of Plasmodium falciparum pfmdr1 alleles following therapy with artemether-lumefantrine in an area of Uganda where malaria is highly endemic. Antimicrob. Agents Chemother. 50 (2006) 1893-1895
3. Duraisingh M.T., Jones P., Sambou I., von Seidlein L., Pinder M., and Warhurst D.C. The tyrosine-86 allele of the pfmdr1 gene of Plasmodium falciparum is associated with increased sensitivity to the anti-malarials mefloquine and artemisinin. Mol. Biochem. Parasitol. 108 (2000) 13-23
4. Hastings I.M., and Ward S.A. Coartem (Artemether-Lumefantrine) in Africa: the beginning of the end?. J. Infect. Dis. 192 (2005) 1303-1304
5. Jambou R., Legrand E., Niang M., Khim N., Lim P., Volney B., Ekala M.T., Bouchier C., Esterre P., Fandeur T., and Mercereau-Puijalon O.S. Resistance of Plasmodium falciparum field isolates to in-vitro artemether and point mutations of the SERCA-type PfATPase6. Lancet 366 (2005) 1960-1963
6. Johnson J.D., Dennull R.A., Gerena L., Lopez-Sanchez M., Roncal N.E., and Waters N.C. Assessment and continued validation of the malaria Sybr Green-I based fluorescence assay for use in malaria drug screening. Antimicrob. Agents Chemother. 51 (2007) 1926-1933
7. Nair S., Nash D., Sudimack D., Jaidee A., Barends M., Uhlemann A.C., Krishna S., Nosten F., and Anderson T.J. Recurrent gene amplification and soft selective sweeps during evolution of multidrug resistance in malaria parasites. Mol. Biol. Evol. 24 (2007) 562-573
8. Nkhoma S., Molyneux M., and Ward S. In vitro antimalarial susceptibility profile and pfcrt/pfmdr-1 genotypes of Plasmodium falciparum field isolates from Malawi. Am. J. Trop. Med. Hyg. 76 6 (2007) 1107-1112
9. Pickard A.L., Wongsrichanalai C., Purfield A., Kamwendo D., Emery K., Zalewski C., Kawamoto F., Miller R.S., and Meshnick S.R. Resistance to antimalarials in Southeast Asia and genetic polymorphisms in pfmdr1. Antimicrob. Agents. Chemother. 47 (2003) 2418-2423
10. Price R.N., Cassar C., Brockman A., Duraisingh M., van Vugt M., White N.J., Nosten F., and Krishna S. The pfmdr1 gene is associated with a multidrug-resistant phenotype in Plasmodium falciparum from the western border of Thailand. Antimicrob. Agents. Chemother. 43 (1999) 2943-2949
11. Price R.N., Uhlemann A.C., Uhlemann A.C., Brockman A., McGready R., Ashley E., Phaipun L., Patel R., Laing K., Looareesuwan S., White N.J., Nosten F., and Krishna S. Mefloquine resistance in Plasmodium falciparum and increased pfmdr1 gene copy number. Lancet 364 (2004) 438-447
12. Price R.N., Uhlemann A.C., van Vugt M., Brockman A., Hutagalung R., Nair S., Nash D., Singhasivanon P., Anderson T.J., Krishna S., White N.J., and Nosten F. Molecular and pharmacological determinants of the therapeutic response to artemether-lumefantrine in multidrug-resistant Plasmodium falciparum malaria. Clin. Infect. Dis. 42 (2006) 1570-1577
13. Roper C., Pearce R., Nair S., Sharp B., Nosten F., and Anderson T. Intercontinental spread of pyrimethamine-resistant malaria. Science 305 (2004) 1124
14. Sidhu A.B., Uhlemann A.C., Valderramos S.G., Valderramos J.C., Krishna S., and Fidock D.A. Decreasing pfmdr1 copy number in Plasmodium falciparum malaria heightens susceptibility to mefloquine, lumefantrine, halofantrine, quinine and artemisinin. J. Infect. Dis. 194 (2006) 528-535
15. Sisowath C., Stromberg J., Martensson A., Msellem M., Obondo C., Bjorkman A., and Gil J.P. In vivo selection of Plasmodium falciparum pfmdr1 86N coding alleles by artemether-lumefantrine (Coartem). J. Infect. Dis. 191 (2005) 1014-1017
16. Sisowath C., Ferreira P.E., Bustamante L.Y., Dahlstrom S., Martensson A., Bjorkman A., Krishna S., and Gil J.P. The role of pfmdr1 in Plasmodium falciparum tolerance to artemether-lumefantrine in Africa. Trop. Med. Int. Health 12 (2007) 736-742
17. Triglia T., Foote S.J., Kemp D.J., and Cowman A.F. Amplification of the multidrug resistance gene pfmdr1 in Plasmodium falciparum has arisen as multiple independent events. Mol. Cell. Biol. 11 (1991) 5244-5250
18. Uhlemann A.C., Ramharter M., Lell B., Kremsner P.G., and Krishna S. Amplification of Plasmodium falciparum multidrug resistance gene 1 in isolates from Gabon. J. Infect. Dis. 192 (2005) 1830-1835
19. Wilson C.M., Volkman S.K., Thaithong S., Martin R.K., Kyle D.E., Milhous W.K., and Wirth D.F. Amplification of pfmdr1 associated with mefloquine and halofantrine resistance in Plasmodium falciparum from Thailand. Mol. Biochem. Parasitol. 57 (1993) 15-160
20. Wootton J.C., Feng X., Ferdig M.T., Cooper R.A., Mu J., Baruch D.I., Magill A.J., and Su X.Z. Genetic diversity and chloroquine selective sweeps in Plasmodium falciparum. Nature 418 (2002) 320-323