Coexpression of human α- and circularly permuted β-globins yields a hemoglobin with normal R state but modified T state properties

Biochemistry

Volumn 48, Issue 23, 2009, Pages 5456-5465

  Asmundson, Anna L ^a,c   Taber, Alexandria M ^a   Van Der Walde, Adella ^a   Lin, Danielle H ^a   Olson, John S ^b   Anthony Cahill, Spencer J ^a  

^a WESTERN WASHINGTON UNIVERSITY   (United States)

^b RICE UNIVERSITY   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL CELLS; BIMOLECULAR ASSOCIATION; BIPHASIC; CO-EXPRESSION; COEXPRESSED; FLASH PHOTOLYSIS; GEL CHROMATOGRAPHY; LIGANDED HEMOGLOBIN; N-VALUE; OXYGEN BINDING; RAPID MIXING; TIME COURSE;

CHROMATOGRAPHIC ANALYSIS; GELATION; HEMOGLOBIN; OLIGOMERS; OXYGEN; PHOTOLYSIS;

RATE CONSTANTS;

ALPHA GLOBIN; BETA GLOBIN; CARBON DIOXIDE; CIRCULARLY PERMUTED BETA GLOBIN; DEOXYHEMOGLOBIN; HEMOGLOBIN; HEMOGLOBIN A; HEMOGLOBIN ALPHA CHAIN; HEMOGLOBIN BETA CHAIN; LIGAND; OXYGEN; OXYHEMOGLOBIN; PROTEIN SUBUNIT; RECOMBINANT HEMOGLOBIN; UNCLASSIFIED DRUG;

ALPHA CHAIN; ARTICLE; BACTERIAL CELL; BACTERIAL STRAIN; BETA CHAIN; DIMERIZATION; ELECTROSPRAY MASS SPECTROMETRY; ESCHERICHIA COLI; GEL CHROMATOGRAPHY; GENE CONSTRUCT; HEMOGLOBIN DETERMINATION; HUMAN; LIGAND BINDING; MOLECULAR WEIGHT; NONHUMAN; OXYGEN AFFINITY; PHOTOLYSIS; POLYMERIZATION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN STRUCTURE;

ALPHA-GLOBINS; BETA-GLOBINS; BINDING SITES; CARBON MONOXIDE; HEMOGLOBINS; HUMANS; KINETICS; LIGANDS; MODELS, MOLECULAR; OXYGEN; PHOTOLYSIS; PROTEIN CONFORMATION; RECOMBINANT PROTEINS;

BACTERIA (MICROORGANISMS);

EID: 67049134249     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi900216p     Document Type: Article

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