Molecular cloning and expression of a sweet potato cysteine protease SPCP1 from senescent leaves

Botanical Studies

Volumn 50, Issue 2, 2009, Pages 159-170

  Chen, Hsien Jung ^a   Huang, Guan Jhong ^a   Chen, Wei Shan ^b   Su, Cheng Ting ^c   Hou, Wen Chi ^d   Lin, Yaw Huei ^e  

^a NATIONAL SUN YAT SEN UNIVERSITY   (Taiwan)

^b CHINA MEDICAL UNIVERSITY   (Taiwan)

^c CHINESE CULTURE UNIVERSITY   (Taiwan)

^d TAIPEI MEDICAL UNIVERSITY   (Taiwan)

^e INSTITUTE OF PLANT AND MICROBIAL BIOLOGY   (Taiwan)

Author keywords

Cysteine protease; Ethephon; Leaf senescence; SPCP1; Sweet potato

Indexed keywords

ALNUS; ALNUS GLUTINOSA; ARABIDOPSIS; ARABIDOPSIS THALIANA; ASTRAGALUS SINICUS; BRASSICA NAPUS; DAUCUS CAROTA; GOSSYPIUM HIRSUTUM; HORDEUM; HORDEUM VULGARE; IPOMOEA BATATAS; IRIS HOLLANDICA; MEDICAGO TRUNCATULA; NICOTIANA TABACUM; ORYZA SATIVA; RICINUS COMMUNIS; TRIFOLIUM REPENS;

EID: 66749141473     PISSN: 1817406X     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (41)

1. Asp, T., S. Bowra, S. Borg, and P.B. Holm. 2004. Cloning and characterization of three groups of cysteine protease genes expressed in the senescing zones of white clover (Trifolium repens) nodules. Plant Sci. 167: 825-837.
2. Buchanan-Wollaston V. 1997. The molecular biology of leaf senescence. J. Exp. Bot. 48: 181-199.
3. Buchanan-Wollaston, V., T. Page, E. Harrison, E. Breeze, and P.O. Lim. 2005. Comparative transcriptome analysis reveals significanct differences in gene expression and signaling pathways between developmental and dark/starvationinduced senescence in Arabidopsis. Plant J. 42: 567-585.
4. Chen, H.J., W.C. Hou, W.N. Jane, and Y.H. Lin. 2000. Isolation and characterization of an isocitrate lyase gene from senescent leaves of sweet potato (Ipomoea batatas cv. Tainong 57). J. Plant Physiol. 157: 669-676.
5. Chen, H.J., W.C. Hou, J.S. Liu, C.Y. Yang, D.J. Huang, and Y.H. Lin. 2004. Molecular cloning and characterization of a cDNA encoding asparaginyl endopeptidase from sweet potato (Ipomoea batatas (L.) Lam) senescent leaves. J. Exp. Bot. 55: 825-835.
6. Chen, H.J., W.C. Hou, C.Y. Yang, D.J. Huang, J.S. Liu, and Y.H. Lin. 2003. Molecular cloning of two metallothionein-like protein genes with differential expression patterns from sweet potato (Ipomoea batatas (L.) Lam.) leaves. J. Plant Physiol. 160: 547-555.
7. Chen, H.J., G.J. Huang, W.C. Hou, J.S. Liu, and Y.H. Lin. 2006. Molecular cloning and characterization of a granulincontaining cysteine protease SPCP3 from sweet potato (Ipomoea batatas) senescent leaves. J. Plant Physiol. 163: 863-876.
8. Chen, H.J., I.C. Wen, G.J. Huang, W.C. Hou, and Y.H. Lin. 2008. Expression of sweet potato asparaginyl endopeptidase caused altered phenotypic characteristics in transgenic Arabidopsis. Bot. Stud. 49: 109-117.
9. Gietl, C., B. Wimmer, J. Adamec, and F. Kalousek. 1997. A cysteine endopeptidase isolated from castor bean endosperm microbodies processes the glyoxysomal malate dehydrogenase precursor protein. Plant Physiol. 113: 863-871.
10. Goetting-Minesky, M.P. and B.C. Mullin. 1994. Differential gene expression in an actinorhizal symbiosis: Evidence for a nodule-specific cysteine proteinase. Proc. Natl. Acad. Sci. USA 91: 9891-9895.
11. Gombert, J., P. Etienne, A. Ourry, and F.L. Dily. 2006. The expression patterns of SAG12/Cab genes reveal the spatial and temporal progression of leaf senescence in Brassica napus L. with sensitivity to the environment. J. Exp. Bot. 57: 1949-1956.
12. Hattori, T., T. Nakagawa, M. Maeshima, K. Nakamura, and T. Asahi. 1985. Molecular cloning and nucleotide sequence of cDNA for sporamin, the major soluble protein of sweet potato tuberous roots. Plant Mol. Biol. 5: 313-320.
13. Hou, W.C., Y.C. Chen, H.J. Chen, Y. H. Lin, L.L. Yang, and M. H. Lee. 2001. Antioxidant activities of trypsin inhibitor, a 33 kDa root storage protein of sweet potato (Ipomoea batatas (L.) Lam cv. Tainong 57). J. Agri. Food Chem. 49: 2978-2981.
14. Hou, W.C., H.J. Chen, and Y.H. Lin. 2003. Antioxidant peptides with angiotensin converting enzyme inhibitory activities and applications for angiotensin converting enzyme purification. J. Agri. Food Chem. 51: 1706-1709.
15. Huang, D.J., W.C. Hou, H.J. Chen, and Y.H. Lin. 2006. Sweet potato (Ipomoea batatas [L.] Lam 'Tainong 57') storage root mucilage exhibited angiotensin converting enzyme inhibitory activity in vitro. Bot. Stud. 47: 397-402.
16. Huang, G.J., H.J. Chen, Y.S. Chang, M.J. Shue, and Y.H. Lin. 2007a. Recombinant sporamin and its synthesized peptides with antioxidant activities in vitro. Bot. Stud. 48: 133-140.
17. Huang, G.J., M.J. Sheu, H.J. Chen, Y.S. Chang, and Y.H. Lin. 2007b. Inhibition of reactive nitrogen species in vitro and ex vivo by trypsin inhibitor from sweet potato 'Tainong 57' storage roots. J. Agri. Food Chem. 55: 6000-6006.
18. Huang, G.J., M.J. Sheu, H.J. Chen, Y.S. Chang, and Y.H. Lin. 2007c. Growth inhibition and induction of apoptosis in NB4 promyelocytic leukemia cells by trypsin inhibitor from sweet potato storage roots. J. Agri. Food Chem. 55: 2548-2553.
19. Jonson, T., E.H. Mahlamaki, R. Karhu, L. Gorunova, B. Johansson, and M. Huglund. 2000. Characterization of genomically amplified segments using PCR: Optimizing relative-PCR for reliable and simple gene expression and gene copy analyses. Gen. Chrom. Cancer 29: 192-199.
20. Lee, T.M. and Y.H. Lin. 1995. Trypsin inhibitor and trypsin-like protease activity in air- or submergence-grown rice (Oryza sativa L.) coleoptiles. Plant Sci. 106: 43-54.
21. Lichtenthaler, H.K. 1987. Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Meth. Enzym. 148: 350-382.
22. Lim, P.O., H.J. Kim, and H.G. Nam. 2007. Leaf senescence. Annu. Rev. Plant Biol. 58: 115-136.
23. Makino, A. and B. Osmond. 1991. Effects of nitrogen nutrition on nitrogen partitioning between chloroplasts and mitochondria in pea and wheat. Plant Physiol. 96: 355-362.
24. Martinez, D.E., M.L. Costa, F.M. Gomez, M.S. Otegui, and J.J. Guiamet. 2008. "Senescence-associated vacuoles" are involved in the degradation of chloroplast proteins in tobacco leaves. Plant J. (Published Online 28 June, 2008).
25. Marty, F. 1999. Plant vacuoles. Plant Cell 11: 587-599.
26. Mitsuhashi, W., T. Yamashita, T. Toyomasu, Y. Kashiwagi, and T. Konnai. 2004. Sequential development of cysteine proteinase activities and gene expression during somatic embryogenesis in carrot. Biosci. Biotechnol. Biochem. 68: 705-713.
27. Naito, Y., M. Fujie, S. Usami, Y. Murooka, and T. Yamada. 2000. The involvement of a cysteine proteinase in the nodule development in Chinese milk vetch infected with Mesorhizobium huakuii subsp. Rengei. Plant Physiol. 124: 1087-1095.
28. No h , Y.S. and R.M. Amasino . 1999 . Regulation of developmental senescence is conserved between Arabidopsis and Brassica napus. Plant Mol. Biol. 41: 195-206.
29. Okamoto, T. and T. Minamikawa. 1999. Molecular cloning and characterization of Vigna mungo processing enzyme 1 (VmPE-1), an asparaginyl endopeptidase possibly involved in post-translational processing of a vacuolar cysteine endopeptidase (SH-EP). Plant Mol. Biol. 39: 63-73.
30. Otegui, M.S., Y.S. Noh, D.E.Martinez, M.G. Vila Petroff, L.A. Staehelin, R.M. Amasino, and J.J. Guiamet. 2005. Senescence-associated vacuoles with intense proteolytic activity develop in leaves of Arabidopsis and soybean. Plant J. 41: 831-844.
31. Pontier, D., S. Gan, R.M. Amasino, D. Roby, and E. Lam. 1999. Markers for hypersensitive response and sequence show distinct patterns of expression. Plant Mol. Biol. 39: 1243-1255.
32. Pourtau, N., R. Jennings, E. Pelzer, J. Pallas, and A. Wingler. 2006. Effect of sugar-induced senescence on gene expression and implications for the regulation of senescence in Arabidopsis. Planta 224: 556-568.
33. Quirino, B.F., Y.S. Noh, E. Himelblau, and R.M. Amasino. 2000. Molecular aspects of leaf senescence. Trends. Plant Sci. 5: 278-282.
34. Sambrook, J., E.F. Fritsch, and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press.
35. Schlereth, A, D. Standhardt, H.P. Mock, and K. Muntz. 2001. Stored cysteine proteinases start globulin mobilization in protein bodies of embryonic axes and cotyledons during vetch (Vicia sativa L.) seed germination. Planta 212: 718-727.
36. Schmid, M., D. Simpson, F. Kalousek, and C. Gietl. 1998. A cysteine endopeptidase with a C-terminal KDEL motif isolated from castor bean endosperm is a marker enzyme for the ricinosome, a putative lytic compartment. Planta 206: 466-475.
37. Sihachakr, D., R. Haïcour, J.M. Cavalcante Alves, I. Umboh, D. Nzoghé, A. Servaes, and G. Ducreux. 1997. Plant regeneration in sweet potato (Ipomoea batatas L., Convolvulaceae). Euphytica 96: 143-152.
38. Vierstra, R.D. 1996. Proteolysis in plants: mechanisms and functions. Plant Mol. Biol. 32: 275-302.
39. Vincent, J.L., M.R.Knox, T.H. Ellis, P. Kalo, G.B. Kiss, and N.J. Brewin. 2000. Nodule-expressed Cyp15a cysteine protease genes map to syntenic genome regions in Pisum and Medicago spp. Mol. Plant Microbe. Int. 13: 715-723.
40. Yang, T.H., Y.C. Tsai, C.T. Hseu, H.S. Ko, S.W. Chen, and R.Q. Blackwell. 1975. Protein content and its amino acid distribution of locally produced rice and sweet potato in Taiwan. J. Chin. Agri. Chem. Soc. 13: 132-138.
41. Yoshida, S. 2003. Molecular regulation of leaf senescence. Curr. Opin. Plant Biol. 6: 79-84.