Chapter 5 Limbal Stem Cells. Application in Ocular Biomedicine

International Review of Cell and Molecular Biology

Volumn 275, Issue C, 2009, Pages 133-181

  Vemuganti, Geeta K ^a   Fatima, Anees ^a   Madhira, Soundarya Lakshmi ^a   Basti, Surendra ^b   Sangwan, Virender S ^a  

^a L V PRASAD EYE INSTITUTE   (India)

^b NORTHWESTERN UNIVERSITY   (United States)

Author keywords

Clinical application; Cryopreservation; Limbal stem cell deficiency; Limbal stem cells; Microarrays; Proteomics; Tissue engineering

Indexed keywords

CHITOSAN; COLLAGEN TYPE 4; COPOLYMER; FIBRIN; FIBRONECTIN; FLUOROURACIL; IMMUNOSUPPRESSIVE AGENT; LAMININ; MITOMYCIN C;

AIRLIFT REACTOR; ALLOTRANSPLANTATION; AMNION; AUTOIMMUNE DISEASE; BLEPHAROSPASM; CADAVER DONOR; CELL COUNT; CELL LABELING; CELL POPULATION; CELL STRAIN 3T3; CELL STRUCTURE; CELL THERAPY; CHEMICAL INJURY; CHRONIC INFLAMMATION; COLONY FORMING UNIT; CONJUNCTIVA; CONJUNCTIVA EPITHELIUM; CONTACT LENS; CORNEA CELL; CORNEA EPITHELIUM; CORNEA LIMBUS; CORNEA OPACITY; CULTURE MEDIUM; DEBRIDEMENT; DIABETES MELLITUS; DISEASE CLASSIFICATION; DRY EYE; ENDOMETRIUM CELL; EPIPHORA; EYE BURN; EYE DISEASE; EYE INFLAMMATION; EYE PAIN; EYE SURGERY; EYELID DISEASE; GENE EXPRESSION; GRAFT REJECTION; HUMAN; HUMAN CELL CULTURE; IATROGENIC DISEASE; IMMUNOPHENOTYPING; IMMUNOSUPPRESSIVE TREATMENT; KERATITIS; KERATOPATHY; LENS CAPSULE; LIMBAL STEM CELL DEFICIENCY; LIMBAL STEM CELL TRANSPLANTATION; LIMBUS STEM CELL DEFICIENCY; LIVING DONOR; NEOVASCULARIZATION (PATHOLOGY); NONHUMAN; ORGAN CULTURE; PHOTOPHOBIA; PRIORITY JOURNAL; PROTEOMICS; QUALITY CONTROL; RADIATION INJURY; REVIEW; STEM CELL; STEVENS JOHNSON SYNDROME; SUSPENSION CELL CULTURE; TEAR FILM; TISSUE ENGINEERING; TISSUE TRANSPLANTATION; VISUAL IMPAIRMENT; ANIMAL; CELL TRANSPLANTATION; CORNEA DISEASE; CYTOLOGY; EPITHELIUM CELL; STEM CELL TRANSPLANTATION; TRANSPLANTATION;

ANIMALS; CELL TRANSPLANTATION; CORNEAL DISEASES; EPITHELIAL CELLS; EPITHELIUM, CORNEAL; HUMANS; LIMBUS CORNEAE; STEM CELL TRANSPLANTATION; STEM CELLS;

EID: 66149114064     PISSN: 19376448     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S1937-6448(09)75005-1     Document Type: Review

References (174)

1. Adachi W., Ulanovsky H., Li Y., Norman B., Davis J., and Piatigorsky J. Serial analysis of gene expression (SAGE) in the rat limbal and central corneal epithelium. Invest. Ophthalmol. Vis. Sci. 47 (2006) 3801-3810
2. Ahmad S., Kolli S., Li D.Q., de Paiva C.S., Pryzborski S., Dimmick I., Armstrong L., Figueiredo F.C., and Lako M. A putative role for RHAMM/HMMR as a negative marker of stem cell-containing population of human limbal epithelial cells. Stem Cells 26 (2008) 1609-1619
3. Ang L.P., Nakamura T., Inatomi T., Sotozono C., Koizumi N., Yokoi N., and Kinoshita S. Autologous serum-derived cultivated oral epithelial transplants for severe ocular surface disease. Arch. Ophthalmol. 124 (2006) 1543-1551
4. Ang L.P., Sotozono C., Koizumi N., Suzuki T., Inatomi T., and Kinoshita S. A comparison between cultivated and conventional limbal stem cell transplantation for Stevens-Johnson syndrome. Am. J. Ophthalmol. 143 (2007) 178-180
5. Argüeso P., Tisdale A., Spurr-Michaud S., Sumiyoshi M., and Gipson I.K. Mucin characteristics of human corneal-limbal epithelial cells that exclude the rose bengal anionic dye. Invest. Ophthalmol. Vis. Sci. 47 (2006) 113-119
6. Baharvand H., Heidari M., Ebrahimi M., Valadbeigi T., and Salekdeh G.H. Proteomic analysis of epithelium-denuded human amniotic membrane as a limbal stem cell niche. Mol. Vis. 13 (2007) 1711-1721
7. Barrandon Y., and Green H. Three clonal types of keratinocyte with different capacities for multiplication. Proc. Natl. Acad. Sci. USA. 84 (1987) 2302-2306
8. Bickenbach J.R. Identification and behavior of label-retaining cells in oral mucosa and skin. J. Dent. Res. 60 (1981) 1611-1620
9. Bickenbach J.R., and Chism E. Selection and extended growth of murine epidermal stem cells in culture. Exp. Cell. Res 10 (1998) 184-195
10. Boyce S.T., and Ham R.G. Calcium-regulated differentiation of normal human epidermal keratinocytes in chemically defined clonal culture and serum-free serial culture. J. Invest. Dermatol. 81 (1983) 33s-40s
11. Calonge M., Diebold Y., Sáez V., Enríquez de Salamanca A., García-Vázquez C., Corrales R.M., and Herreras J.M. Impression cytology of the ocular surface: A review. Exp. Eye Res. 78 (2004) 457-472
12. Capuco A.V. Identification of putative bovine mammary epithelial stem cells by their retention of labeled DNA strands. Exp. Biol. Med.(Maywood) 232 (2007) 1381-1390
13. Cauchi P.A., Ang G.S., Azuara-Blanco A., and Burr J.M. A systematic literature review of surgical interventions for limbal stem cell deficiency in humans. Am. J. Ophthalmol. 146 (2008) 251-259
14. Chen J.J., and Tseng S.C. Corneal wound healing in partial limbal deficiency. Invest. Ophthalmol. Vis. Sci. 31 (1990) 1301-1314
15. Chen J.J., and Tseng S.C. Abnormal corneal epithelial wound healing in partial-thickness removal of limbal epithelium. Invest. Ophthalmol. Vis. Sci. 32 (1991) 2219-2233
16. Chen Y.T., Li W., Hayashida Y., He H., Chen S.Y., Tseng D.Y., Kheirkhah A., and Tseng S.C. Human amniotic epithelial cells as novel feeder layers for promoting ex vivo expansion of limbal epithelial progenitor cells. Stem Cells 25 (2007) 1995-2005
17. Collinson J.M., Morris L., Reid A.I., Ramaesh T., Keighren M.A., Flockhart J.H., Hill R.E., Tan S.S., Ramaesh K., Dhillon B., and West J.D. Clonal analysis of patterns of growth, stem cell activity, and cell movement during the development and maintenance of the murine corneal epithelium. Dev. Dyn. 224 (2002) 432-440
18. Conboy M.J., Karasov A.O., and Rando T.A. High incidence of non-random template strand segregation and asymmetric fate determination in dividing stem cells and their progeny. PLoS Biol. 5 (2007) e102
19. Coster D.J., Aggarwal R.K., and Williams K.A. Surgical management of ocular surface disorders using conjunctival and stem cell allografts. Br. J. Ophthalmol. 79 (1995) 977-982
20. Cotsarelis G., Cheng S.Z., Dong G., Sun T.T., and Lavker R.M. Existence of slow-cycling limbal epithelial basal cells that can be preferentially stimulated to proliferate: Implications on epithelial stem cells. Cell 57 (1989) 201-209
21. Cotsarelis G., Kaur P., Dhouailly D., Hengge U., and Bickenbach J. Epithelial stem cells in the skin: Definition, markers, localization andfunctions. Exp. Dermatol. 8 (1999) 80-88
22. Croasdale C.R., Schwartz G.S., Malling J.V., and Holland E.J. Keratolimbal allograft: Recommendations for tissue procurement and preparation by eye banks, and standard surgical technique. Cornea 18 (1999) 52-58
23. Davanger M., and Evensen A. Role of the pericorneal papillary structure in renewal of corneal epithelium. Nature 229 (1971) 560-561
24. Daya S.M., Bell R.W., Habib N.E., Powell-Richards A., and Dua H.S. Clinical and pathologic findings in human keratolimbal allograft rejection. Cornea 19 (2000) 443-450
25. Di Girolamo N., Chui J., Wakefield D., and Coroneo M.T. Cultured human ocular surface epithelium on therapeutic contact lenses. Br. J. Ophthalmol. 91 (2007) 459-464
26. Di Girolamo N., Sarris M., Chiu J., Cheema H., Coroneo M.T., and Wakefield D. Localisation of the low affinity nerve growth factor receptor p75 in human limbal epithelial cells. J. Cell Mol. Med. 12 (2009) 2799-2811
27. Djalilian A.R., Mahesh S.P., Koch C.A., Nussenblatt R.B., Shen D., Zhuang Z., Holland E.J., and Chan C.C. Survival of donor epithelial cells after limbal stem cell transplantation. Invest Ophthalmol Vis Sci. 46 (2005) 803-807
28. Doillon C.J., Watsky M.A., Hakim M., Wang J., Munger R., Laycock N., Osborne R., and Griffith M. A collagen-based scaffold for a tissue engineered human cornea: Physical and physiological properties. Int. J. Artif. Organs. 26 (2003) 764-773
29. Dravida S., Gaddipati S., Griffith M., Merrett K., Lakshmi Madhira S., Sangwan V.S., and Vemuganti G.K. A biomimetic scaffold for culturing limbal stem cells: A promising alternative for clinical transplantation. J. Tissue Eng. Regen. Med. 2 (2008) 263-271
30. Dua H.S. The conjunctiva in corneal epithelial wound healing. Br. J. Ophthalmol. 82 (1998) 1407-1411
31. Dua H.S., and Azuara-Blanco A. Limbal stem cells of the corneal epithelium. Surv. Ophthalmol. 44 (2000) 415-425
32. Dua H.S., and Forrester H.V. The corneo-scleral limbus in human corneal epithelial wound healing. Am. J. Ophthalmol. 110 (1990) 646-656
33. Dua H.S., Gomes J.A., and Singh A. Corneal epithelial wound healing. Br. J. Ophthalmol. 78 (1994) 401-408
34. Ebato B., Friend J., and Thoft R.A. Comparison of central and peripheral human corneal epithelium in tissue culture. Invest. Ophthalmol. Vis. Sci. 28 (1987) 1450-1456
35. Elder M.J., Hiscott P., and Dart J.K. Intermediate filament expression by normal and diseased human corneal epithelium. Hum. Pathol. 28 (1997) 1348-1354
36. Epstein S.P., Wolosin J.M., and Asbell P.A. P63 expression levels in side population and low light scattering ocular surface epithelial cells. Trans. Am. Ophthalmol. Soc. 103 (2005) 187-199 discussion 199
37. Espana E., Sandoval H., Ti S.E., and Goto E. Ex vivo preservation and expansion of human limbal epithelial stem cells on amniotic membrane for treating corneal diseases with total limbal stem cell deficiency. Adv. Exp. Med. Biol. 506 (2002) 1323-1334
38. Espana E.M., Kawakita T., Romano A., Pascuale M.D., Smiddy R., Liu C., and Tseng S.G. Stromal niche controls the plasticity of limbal and corneal epithelial differentiation in a rabbit model of recombined tissue. Invest. Ophthalmol. Vis. Sci. 44 (2003) 5130-5135
39. Espana E.M., Di Pascuale M.A., He H., Kawakita T., Raju V.K., Liu C.Y., and Tseng S.C. Characterization of corneal pannus removed from patients with total limbal stem cell deficiency. Invest. Ophthalmol. Vis. Sci. 45 (2004) 2961-2966
40. Fatima A., Sangwan V.S., Iftekhar G., Reddy P., Matalia H., Balasubramanian D., and Vemuganti G.K. Technique of cultivating limbal derived corneal epithelium on human amniotic membrane for clinical transplantation. J. Postgrad. Med. 52 (2006) 257-261
41. Fatima A., Vemuganti G.K., Iftekhar G., Rao G.N., and Sangwan V.S. In vivo survival and stratification of cultured limbal epithelium. Clin. Exp. Ophthalmol. 35 (2007) 96-98
42. Fatima A., Iftekhar G., Sangwan V.S., and Vemuganti G.K. Ocular surface changes in limbal stem cell deficiency caused by chemical injury: A histologic study of excised pannus from recipients of cultured corneal epithelium. Eye 22 (2008) 1161-1167
43. Figueira E.C., Di Girolamo N., Coroneo M.T., and Wakefield D. The phenotype of limbal epithelial stem cells. Invest. Ophthalmol. Vis. Sci. 48 (2007) 144-156
44. Foreman D.M., Pancholi S., Jarvis-Evans J., McLeod D., and Boulton M.E. A simple organ culture model for assessing the effects of growth factors on corneal re-epithelialization. Exp. Eye Res. 62 (1996) 555-564
45. Francis D., Abberton K., Thompson E., and Daniell M. Myogel supports the ex-vivo amplification of corneal epithelial cells. Exp. Eye Res. 88 (2009) 339-346
46. Fujishima H., Shimazaki J., and Tsubota K. Temporary corneal stem cell dysfunction after radiation therapy. Br. J. Ophthalmol. 80 (1996) 911-914
47. Galal A., Perez-Santonja J.J., Rodriguez-Prats J.L., Abad M., and Alio J. Human anterior lens capsule as a biologic substrate for the ex vivo expansion of limbal stem cells in ocular surface reconstruction. Cornea 26 (2007) 473-478
48. Griffith M., Osborne R., Munger X.X., Doillon C., Laycock N.L.C., Hakim M., Song Y., and Watsky M.A. Functional human corneal equivalents from cell lines. Science 286 (1999) 2169-2172
49. Grueterich M., and Tseng S.C. Human limbal progenitor cells expanded on intact amniotic membrane ex vivo. Arch. Ophthalmol. 120 (2002) 783-790
50. Grueterich M., Espana E., and Tseng S.C. Connexin 43 expression and proliferation of human limbal epithelium on intact and denuded amniotic membrane. Invest Ophthalmol Vis Sci. 43 (2002) 63-71
51. Grueterich M., Espana E.M., and Tseng S.C. Modulation of keratin and connexin expression in limbal epithelium expanded on denuded amniotic membrane with and without a 3T3 fibroblast feeder layer. Invest. Ophthalmol. Vis. Sci. 44 (2003) 4230-4236
52. Hanna C. Proliferation and migration of epithelial cells during corneal wound repair in the rabbit and the rat. Am. J. Ophthalmol 61 (1966) 55-63
53. 3H]thymidine labels less than half of the DNA-synthesizing cells in the mouse tumour, carcinoma NT. Cell Tissue Kinet. 15 (1982) 405-411
54. Hayashida Y., Nishida K., Yamato M., Watanabe K., Maeda N., Watanabe H., Kikuchi A., Okano T., and Tano Y. Ocular surface reconstruction using autologous rabbit oral mucosal epithelial sheets fabricated ex vivo on a temperature-responsive culture surface. Invest. Ophthalmol. Vis. Sci. 46 (2005) 1632-1639
55. Henderson T.R., McCall S.H., Taylor G.R., and Noble B.A. Do transplanted corneal limbal stem cells survive in vivo long-term? Possible techniques to detect donor cell survival by polymerase chain reaction with the amelogenin gene and Y-specific probes. Eye 11 (1997) 779-785
56. Henderson T.R., Findlay I., Matthews P.L., and Noble B.A. Identifying the origin of single corneal cells by DNA fingerprinting: Part II-application to limbal allografting. Cornea 20 (2001) 404-407
57. Higa K., Shimmura S., Kato N., Kawakita T., Miyashita H., Itabashi Y., Fukuda K., Shimazaki J., and Tsubota K. Proliferation and differentiation of transplantable rabbit epithelial sheets engineered with or without an amniotic membrane carrier. Invest. Ophthalmol. Vis. Sci. 48 (2007) 597-604
58. Holbrook K.A., and Odland G.F. The fine structure of developing human epidermis: Light, scanning, and transmission electron microscopy of the periderm. J. Invest. Dermatol. 65 (1975) 16-38
59. Holland E.J., and Schwartz G.S. The evolution of epithelial transplantation for severe ocular surface disease and a proposed classification system. Cornea 15 (1996) 549-556
60. Holland E.J., and Schwartz G.S. Iatrogenic limbal stem cell deficiency. Trans. Am. Ophthalmol. Soc. 95 (1997) 95-107 discussion 107-110
61. Homma R., Yoshikawa H., Takeno M., Kurokawa M.S., Masuda C., Takada E., Tsubota K., Ueno S., and Suzuki N. Induction of epithelial progenitors in vitro from mouse embryonic stem cells and application for reconstruction of damaged cornea in mice. Invest. Ophthalmol. Vis. Sci. 45 (2004) 4320-4326
62. Houseman B.T., Huh J.H., Kron S.J., and Mrksich M. Peptide chips for the quantitative evaluation of protein kinase activity. Nat. Biotechnol. 20 (2002) 270-274
63. Ilari L., and Daya S.M. Long-term outcomes of keratolimbal allograft for the treatment of severe ocular surface disorders. Ophthalmology 109 (2002) 1278-1284
64. James S.E., Rowe A., Ilari L., Daya S., and Martin R. The potential for eye bank limbal rings to generate cultured corneal epithelial allografts. Cornea 20 (2001) 488-494
65. + cell enumeration in blood and leukapheresis products: Report from the second Nordic Workshop. Nordic Stem Cell Laboratory Group (NSCL-G). J. Hematother. 5 (1996) 237-245
66. Jones P.H., and Watt F.M. Separation of human epidermal stem cells from transit amplifying cells on the basis of differences in integrin function and expression. Cell 73 (1993) 713-724
67. Joseph A., Powell-Richards A.O., Shanmuganathan V.A., and Dua H.S. Epithelial cell characteristics of cultured human limbal explants. Br. J. Ophthalmol. 88 (2004) 393-398
68. Kawasaki S., Tanioka H., Yamasaki K., Yokoi N., Komuro A., and Kinoshita S. Clusters of corneal epithelial cells reside ectopically in human conjunctival epithelium. Invest. Ophthalmol. Vis. Sci. 47 (2006) 1359-1367
69. Kenyon K.R., and Tseng S.C. Limbal autograft transplantation for ocular surface disorders. Ophthalmology 96 (1989) 709-722 discussion 722-723
70. Kim J.C., and Tseng S.C. Transplantation of preserved human amniotic membrane for surface reconstruction in severely damaged rabbit corneas. Cornea 14 (1995) 473-484
71. Kim H.S., Jun Song X., de Paiva C.S., Chen Z., Pflugfelder S.C., and Li D.Q. Phenotypic characterization of human corneal epithelial cells expanded ex vivo from limbal explant and single cell cultures. Exp. Eye Res. 79 (2004) 41-49
72. Kinoshita S., Kiritoshi A., Ohji M., Ohashi Y., and Manabe R. Disappearance of palisades of Vogt in ocular surface disease. Jpn. J. Clin. Ophthalmol. 40 (1986) 363-366
73. Koizumi N., Inatomi T., Suzuki T., Sotozono C., and Kinoshita S. Cultivated corneal epithelial stem cell transplantation in ocular surface disorders. Ophthalmology 108 (2001) 1569-1574
74. Koizumi N., Cooper L.J., Fullwood N.J., Nakamura T., Inoki K., Tsuzuki M., and Kinoshita S. An evaluation of cultivated corneal limbal epithelial cells, using cell-suspension culture. Invest. Ophthalmol. Vis. Sci. 43 (2002) 2114-2121
75. Koizumi N., Rigby H., Fullwood N.J., Kawasaki S., Tanioka H., Koizumi K., Kociok N., Joussen A.M., and Kinoshita S. Comparison of intact and denuded amniotic membrane as a substrate for cell-suspension culture of human limbal epithelial cells. Graefes Arch. Clin. Exp. Ophthalmol. 245 (2007) 123-134
76. Kolli S., Lako M., Figueiredo F., Mudhar H., and Ahmad S. Loss of corneal epithelial stem cell properties in outgrowths from human limbal explants cultured on intact amniotic membrane. Regen. Med. 3 (2008) 329-342
77. Kruse E.F. Stem cells and corneal regeneration. Eye 8 (1994) 170-183
78. Kruse F.E., Chen J.J., Tsai R.J., and Tseng S.C. Conjunctival transdifferentiation is due to the incomplete removal of limbal basal epithelium. Invest. Ophthalmol. Vis. Sci. 31 (1990) 1903-1913
79. Lajtha L.G. Haemopoietic stem cells: Concept and definitions. Blood Cells 5 (1979) 447-455
80. Lavker R.M., and Sun T.T. Heterogeneity in epidermal basal keratinocytes: Morphological and functional correlations. Science 215 (1982) 1239-1241
81. Lavker R.M., and Sun T.T. Epidermal stem cells: Properties, markers, and location. Proc. Natl. Acad. Sci. USA. 97 (2000) 13473-13475
82. Lavker R.M., Dong G., Cheng S.Z., Kudoh K., Cotsarelis G., and Sun T.T. Relative proliferative rates of limbal and corneal epithelia. Implications of corneal epithelial migration, circadian rhythm, and suprabasally located DNA-synthesizing keratinocytes. Invest. Ophthalmol. Vis. Sci. 32 (1991) 1864-1875
83. Leblond C.P. The life history of cells in renewing systems. Am. J. Anat. 160 (1981) 114-158
84. Lee S.H., and Tseng S.C. Amniotic membrane transplantation for persistent epithelial defects with ulceration. Am. J. Ophthalmol. 123 (1997) 303-312
85. Lee J.B., Song J.M., Lee J.E., Park J.H., Kim S.J., Kang S.M., Kwon J.N., Kim M.K., Roh S.I., and Yoon H.S. Available human feeder cells for the maintenance of human embryonic stem cells. Reproduction 128 (2003) 727-735
86. Lehrer M.S., Sun T.T., and Lavker R.M. Strategies of epithelial repair: Modulation of stem cell and transit amplifying cell proliferation. J. Cell Sci. 111 (1998) 2867-2875
87. Lemp M.A. Report of the national eye institute/industry workshop on clinical trials in dry eyes. CLAO J 21 (1995) 221-232
88. Li F., Carlsson D.J., Lohmann C.P., Suuronen E.J., Vascotto S., Kobuch K., Sheardown H., Munger M., and Griffith M. Cellular and nerve regeneration within a biosynthetic extracellular matrix: Corneal implantation. Proc. Natl. Acad. Sci. USA 100 (2003) 15346-15351
89. Li D.Q., Chen Z., Song X.J., de Paiva C.S., Kim H.S., and Pflugfelder S.C. Partial enrichment of a population of human limbal epithelial cells with putative stem cell properties based on collagen type IV adhesiveness. Exp. Eye Res. 80 (2005) 581-590
90. Lindberg K., Brown M.E., Chaves H.V., Kenyon K.R., and Rheinwald J.G. In vitro propagation of human ocular surface epithelial cells for transplantation. Invest. Ophthalmol. Vis. Sci. 34 (1993) 2672-2679
91. Ma D.H., Chen J.K., Zhang F., Lin K.Y., Yao J.Y., and Yu J.S. Regulation of corneal angiogenesis in limbal stem cell deficiency. Prog. Retin. Eye Res. 25 (2006) 563-590
92. Madhavan H.N., Malathi J., Joseph R.P., Mori Y., Abraham S.J.K., and Yoshioka H. A study on the growth of continuous culture cell lines embedded in Mebiol Gel. Curr. Sci. 87 (2004) 1275-1277
93. Madhira S.L., Vemuganti G., Bhaduri A., Gaddipati S., Sangwan V.S., and Ghanekar Y. Culture and characterization of oral mucosal epithelial cells on human amniotic membrane for ocular surface reconstruction. Mol. Vis. 14 (2008) 189-196
94. Meller D., and Tseng S.C. Conjunctival epithelial cell differentiation on amniotic membrane. Invest. Ophthalmol. Vis. Sci. 40 (1999) 878-886
95. Meller D., Dabul V., and Tseng S.C. Expansion of conjunctival epithelial progenitor cells on amniotic membrane. Exp. Eye Res. 74 (2002) 537-545
96. Mi S., Yang X., Zhao Q., Qu L., Chen S., M Meek K., and Dou Z. Reconstruction of corneal epithelium with cryopreserved corneal limbal stem cells in a goat model. Mol. Reprod. Dev. 75 (2008) 1607-1616
97. Moore J.E., McMullen C.B., Mahon G., and Adamis A.P. The corneal epithelial stem cell. DNA Cell Biol. 21 (2002) 443-451
98. Morris R.J., Fischer S.M., Klein-Szanto A.J., and Slaga T.J. Subpopulations of primary adult murine epidermal basal cells sedimented on density gradients. Cell Tissue Kinet. 23 (1990) 587-602
99. Nakagawa S., Nishida T., Kodama Y., and Itoi M. Spreading of cultured corneal epithelial cells on fibronectin and other extracellular matrices. Cornea 9 (1990) 125-130
100. Nelson D.J. Impression cytology. Cornea 7 (1988) 71-81
101. Nishida K., Yamato M., Hayashida Y., Watanabe K., Maeda N., Watanabe H., Yamamoto K., Nagai S., Kikuchi A., Tano Y., and Okano T. Functional bioengineered corneal epithelial sheet grafts from corneal stem cells expanded ex vivo on a temperature-responsive cell culture surface. Transplantation 77 (2004) 379-385
102. Nishida K., Yamato M., Hayashida Y., Watanabe K., Yamamoto K., Adachi E., Nagai S., Kikuchi A., Maeda N., Watanabe H., Okano T., and Tano Y. Corneal reconstruction with tissue-engineered cell sheets composed of autologous oral mucosal epithelium. N. Engl. J. Med. 351 (2004) 1187-1196
103. Nishiwaki-Dantas C.M., Dantas P.E.C., and Reggi J.R.A. Ipsilateral limbal translocation for treatment of partial limbal deficiency secondary to ocular alkali burn. Br. J. Ophthalmol. 85 (2001) 1031-1033
104. Notara M., Haddow D.B., MacNeil S., and Daniels J.T. A xenobiotic-free culture system for human limbal epithelial stem cells. Regen. Med. 2 (2007) 919-927
105. Ono K., Yokoo S., Mimura T., Usui T., Miyata K., Araie M., Yamagami S., and Amano S. Autologous transplantation of conjunctival epithelial cells cultured on amniotic membrane in a rabbit model. Mol. Vis. 13 (2007) 1138-1143
106. Pan Z., Zhang W., and Sun B. [A study on proliferation and differentiation of limbal stem cells]. Zhonghua Yan Ke Za Zhi. 35 (1999) 19-21
107. Pan Z., Zhang W., and Wu Y. [An experimental study on treatment of limbal alkali burn by allograft transplantation with cultured stem cells on amniotic membrane]. Zhonghua Yan Ke Za Zhi. 36 (2000) 32-53
108. Park K.S., Lim C.H., Min B.M., Lee J.L., Chung H.Y., Joo C.K., Park C.W., and Son Y. The side population cells in the rabbit limbus sensitively increased in response to the central cornea wounding. Invest. Ophthalmol. Vis. Sci. 47 (2006) 892-900
109. Pavlovitch J.H., Rizk-Rabin M., Jaffray P., Hoehn H., and Poot M. Characteristics of homogeneously small keratinocytes from newborn rat skin: Possible epidermal stem cells. Am. J. Physiol. 261 (1991) C964-C972
110. Pellegrini G., Traverso C.E., Franzi A.T., Zingirian M., Cancedda R., and De Luca M. Long-term restoration of damaged corneal surfaces with autologous cultivated corneal epithelium. Lancet 349 (1997) 990-993
111. Pellois J.P., Zhou X., Srivannavit O., Zhou T., Gulari E., and Gao X. Individually addressable parallel peptide synthesis on microchips. Nat. Biotechnol. 20 (2002) 922-926
112. Pfister R.R. Corneal stem cell disease: Concepts, categorization, and treatment by auto- and homotransplantation of limbal stem cells. CLAO J 20 (1994) 64-72
113. Polisetty N., Fatima A., Madhira S.L., Sangwan V.S., and Vemuganti G.K. Mesenchymal cells from limbal stroma of human eye. Mol. Vis. 14 (2008) 431-442
114. Puangsricharern V., and Tseng S.C. Cytologic evidence of corneal disease with limbal stem cell deficiency. Ophthalmology 102 (1995) 1476-1485
115. Qi H., Chuang E.Y., Yoon K.C., de Paiva C.S., Shine H.D., Jones D.B., Pflugfelder S.C., and Li D.Q. Patterned expression of neurotrophic factors and receptors in human limbal and corneal regions. Mol. Vis. 13 (2007) 1934-1941
116. Qi H., Li D.Q., Shine H.D., Chen Z., Yoon K.C., Jones D.B., and Pflugfelder S.C. Nerve growth factor and its receptor trka serve as potential markers for human corneal epithelial progenitor cells. Exp. Eye Res. 86 (2008) 34-40
117. Qu L., Yang X., Wang X., Zhao M., Mi S., Dou Z., and Wang H. Reconstruction of corneal epithelium with cryopreserved corneal limbal stem cells in a rabbit model. Vet. J. 179 (2009) 392-400
118. Rama P., Bonini S., Lambiase A., Golisano O., Paterna P., De Luca M., and Pellegrini G. Autologous fibrin-cultured limbal stem cells permanently restore the corneal surface of patients with total limbal stem cell deficiency. Transplantation 72 (2001) 1478-1485
119. Rama P., Giannini R., Bruni A., Gatto C., Tiso R., and Ponzin D. Further evaluation of amniotic membrane banking for transplantation in ocular surface diseases. Cell Tissue Bank 2 (2001) 155-163
120. Rao S.K., Rajagopal R., Sitalaxmi G., and Padmanabhan P. Limbal autografting: Comparison of results in acute and chronic phases of ocular surface burns. Cornea 18 (1999) 164-171
121. Rheinwald J.G., and Green H. Serial cultivation of strains of human epidermal keratinocytes: The formation of keratinizing colonies from single cells. Cell 6 (1975) 331-343
122. Sangwan V.S., Vemuganti G.K., Iftekhar G., Bansal A.K., and Rao G.N. Use of autologous cultured limbal and conjunctival epithelium in a patient with severe bilateral ocular surface disease induced by acid injury: A case report of unique application. Cornea 22 (2003) 478-481
123. Sangwan V.S., Matalia H.P., Vemuganti G.K., Ifthekar G., Fatima A., Singh S., and Rao G.N. Early results of penetrating keratoplasty after cultivated limbal epithelium transplantation. Arch. Ophthalmol. 123 (2005) 334-340
124. Sangwan V.S., Ramamurthy B., Shah U., Garg P., Sridhar M.S., and Rao G.N. Outcome of corneal transplant rejection: A 10-year study. Clin. Exp. Ophthalmol. 33 (2005) 623-627
125. Sangwan V.S., Matalia H.P., Vemuganti G.K., Fatima A., Ifthekar G., Singh S., Nutheti R., and Rao G.N. Clinical outcome of autologous cultivated limbal epithelium transplantation. Indian J. Ophthalmol. 54 (2006) 29-34
126. Santos M.S., Gomes J.A., Hofling-Lima A.L., Rizzo L.V., Romano A.C., and Belfort Jr. R. Survival analysis of conjunctival limbal grafts and amniotic membrane transplantation in eyes with total limbal stem cell deficiency. Am. J. Ophthalmol. 140 (2005) 223-230
127. Schermer A., Galvin S., and Sun T.T. Differentiation-related expression of a major 64K corneal keratin in vivo and in culture suggests limbal location of corneal epithelial stem cells. J. Cell Biol. 103 (1986) 49-62
128. Schmaljohann D., Oswald J., Jørgensen B., Nitschke M., Beyerlein D., and Werner C. Thermo-responsive pnipaam-g-PEG films for controlled cell detachment. Biomacromolecules 4 (2003) 1733-1739
129. Schwab I.R., Reyes M., and Isseroff R.R. Successful transplantation of bioengineered tissue replacements in patients with ocular surface disease. Cornea 19 (2000) 421-426
130. Shanmuganathan V.A., Rotchford A.P., Tullo A.B., Joseph A., Zambrano I., and Dua H.S. Epithelial proliferative potential of organ cultured corneoscleral rims; implications for allo-limbal transplantation and eye banking. Br. J. Ophthalmol. 90 (2006) 55-58
131. Shaptron M.S., Friend J., and Thoft R.A. Corneal reepithelisation from conjunctiva. Invest. Ophthalmol. Vis. Sci. 21 (1981) 135-142
132. Shimazaki J., Shimmura S., Ishioka M., and Tsubota K. Randomized clinical trial of deep lamellar keratoplasty vs penetrating keratoplasty. Am. J. Ophthalmol. 134 (2002) 159-165
133. Shimazaki J., Konomi K., Shimmura S., and Tsubota K. Ocular surface reconstruction for thermal burns caused by fireworks. Cornea 25 (2006) 139-145
134. Shimmura S., and Tsubota K. Surgical treatment of limbal stem cell deficiency: Are we really transplanting stem cells?. Am. J. Ophthalmol. 146 (2008) 154-155
135. Shimmura S., Miyashita H., Higa K., Yoshida S., Shimazaki J., and Tsubota K. Proteomic analysis of soluble factors secreted by limbal fibroblasts. Mol. Vis. 12 (2006) 478-484
136. Shinohara T., Orwig K.E., Avarbock M.R., and Brinster R.L. Spermatogonial stem cell enrichment by multiparameter selection of mouse testis cells. Proc. Natl. Acad. Sci. USA. 97 (2000) 8346-8351
137. Shortt A.J., Secker G.A., Lomas R.J., Wilshaw S.P., Kearney J.N., Tuft S.J., and Daniels J.T. The effect of amniotic membrane preparation method on its ability to serve as a substrate for the ex-vivo expansion of limbal epithelial cells. Biomaterials 30 (2009) 1056-1065
138. Sitalakshmi G., Sudha B., Madhavan H.N., Vinay S., Krishnakumar S., Mori Y., Yoshioka H., and Abraham S. Ex vivo cultivation of corneal limbal epithelial cells in a thermoreversible polymer (Mebiol Gel) and their transplantation in rabbits: An animal model. Tissue Eng. Part A. (2008) Aug 26. Epub ahead of print
139. Srinivasan B.D., and Eakins K.E. The reepithelialization of rabbit cornea following single and multiple denudation. Exp. Eye Res. 29 (1979) 595-600
140. Sudha B., Madhavan H.N., Sitalakshmi G., Malathi J., Krishnakumar S., Mori Y., Yoshioka H., and Abraham S. Cultivation of human corneal limbal stem cells in Mebiol gel-A thermo-reversible gelation polymer. Indian J. Med. Res. 124 (2006) 655-664
141. Sun T.T., and Green H. Cultured epithelial cells of cornea, conjunctiva and skin: Absence of marked intrinsic divergence of their differentiated states. Nature 269 (1977) 489-493
142. Talbot M., Carrier P., Giasson C.J., Deschambeault A., Guérin S.L., Auger F.A., Bazin R., and Germain L. Autologous transplantation of rabbit limbal epithelia cultured on fibrin gels for ocular surface reconstruction. Mol. Vis. 12 (2006) 65-75
143. Tani H., Morris R.J., and Kaur P. Enrichment for murine keratinocyte stem cells based on cell surface phenotype. Proc. Natl. Acad. Sci. USA. 97 (2000) 10960-10965
144. Tanioka H., Kawasaki S., Yamasaki K., Ang L.P., Koizumi N., Nakamura T., Yokoi N., Komuro A., Inatomi T., and Kinoshita S. Establishment of a cultivated human conjunctival epithelium as an alternative tissue source for autologous corneal epithelial transplantation. Invest. Ophthalmol. Vis. Sci. 47 (2006) 3820-3827
145. Theng J.T., and Tan D.T. Combined penetrating keratoplasty and limbal allograft transplantation for severe corneal burns. Ophthalmic. Surg. Lasers 28 (1997) 765-768
146. Thoft R.A. The role of the limbus in ocular surface maintenance and repair. Acta Ophthalmol. Suppl. 192 (1989) 91-94
147. Thoft R.A., and Friend J. The X, Y, Z hypothesis of corneal epithelial maintenance. Invest. Ophthalmol. Vis. Sci. 24 (1983) 1442-1443
148. Ti S.E., Grueterich M., Espana E.M., Touhami A., Anderson D.F., and Tseng S.C. Correlation of long term phenotypic and clinical outcomes following limbal epithelial transplantation cultivated on amniotic membrane in rabbits. Br. J. Ophthalmol. 88 (2004) 422-427
149. Touhami A., Grueterich M., and Tseng S.C. The role of NGF signaling in human limbal epithelium expanded by amniotic membrane culture. Invest. Ophthalmol. Vis. Sci. 43 (2002) 987-994
150. Touhami A., Grueterich M., and Tseng S.C. The role of NGF signaling in human limbal epithelium expanded by amniotic membrane culture. Invest. Ophthalmol. Vis. Sci. 43 (2007) 987-994
151. Tsai R.J., and Tseng S.C. Human allograft limbal transplantation for corneal surface reconstruction. Cornea 13 (1994) 389-400
152. Tsai R.J., Li L., and Chen J. Reconstruction of damaged corneas by transplantation of autologous limbal epithelial cells(1). Am. J. Ophthalmol. 130 (2000) 543
153. Tsai R.J., Li L.M., and Chen J.K. Reconstruction of damaged corneas by transplantation of autologous limbal epithelial cells. N. Engl. J. Med. 343 (2000) 86-93
154. Tseng S.C. Staging of conjunctival squamous metaplasia by impression cytology. Ophthalmology 92 (1985) 728-733
155. Tseng S.C.G., Prabhasawat P., Barton K., Gray T., and Meller D. Amniotic membrane transplantation with or without limbal allografts for severe ocular surface disorders. Ophthalmology 102 (1995) 1486-1496
156. Tseng S.C., Prabhasawat P., Barton K., Gray T., and Meller D. Amniotic membrane transplantation with or without limbal allografts for corneal surface reconstruction in patients with limbal stem cell deficiency. Arch. Ophthalmol. 116 (1998) 431-441
157. Tsubota K., Satake Y., Ohyama M., Toda I., Takano Y., Ono M., Shinozaki N., and Shimazaki J. Surgical reconstruction of the ocular surface in advanced ocular cicatricial pemphigoid and Stevens-Johnson syndrome. Am. J. Ophthalmol. 122 (1996) 38-52
158. Tsubota K., Satake Y., Kaido M., Shinozaki N., Shimmura S., Bissen-Miyajima H., and Shimazaki J. Treatment of severe ocular-surface disorders with corneal epithelial stem-cell transplantation. N. Engl. J. Med. 340 (1999) 1697-1703
159. Tungsiripat T., Sarayba M.A., Taban M., Sweet P.M., Osann K.E., and Chuck R.S. Viability of limbal epithelium after anterior lamellar harvesting using a microkeratome. Ophthalmology 111 (2004) 469-475
160. Umemoto T., Yamato M., Nishida K., Yang J., Tano Y., and Okano T. Limbal epithelial side-population cells have stem cell-like properties, including quiescent state. Stem Cells 24 (2006) 86-94
161. Van Buskirk E.M. The anatomy of the limbus. Eye 3 (1989) 101-108
162. Vascotto S.G., and Griffith M. Localization of candidate stem and progenitor cell markers within the human cornea, limbus, and bulbar conjunctiva in vivo and in cell culture. Anat. Rec. A Discov. Mol. Cell Evol. Biol. 288 (2006) 921-931
163. Vemuganti G.K., Kashyap S., Sangwan V.S., and Singh S. Ex-vivo potential of cadaveric and fresh limbal tissues to regenerate cultured epithelium. Indian J. Ophthalmol. 52 (2004) 113-120
164. Wang D.Y., Hsueh Y.J., Yang V.C., and Chen J.K. Propagation and phenotypic preservation of rabbit limbal epithelial cells on amniotic membrane. Invest. Ophthalmol. Vis. Sci. 44 (2003) 4698-4704
165. Wei Z.G., Cotsarelis G., Sun T.T., and Lavker R.M. Label-retaining cells are preferentially located in fornical epithelium: Implications on conjunctival epithelial homeostasis. Invest. Ophthalmol. Vis. Sci. 36 (1995) 236-246
166. Williams K.A., Brereton H.M., Aggarwal R., Sykes P.J., Turner D.R., Russ G.R., and Coster D.J. Use of DNA polymorphisms and the polymerase chain reaction to examine the survival of a human limbal stem cell allograft. Am. J. Ophthalmol. 120 (1995) 342-350
167. Wylegała E.A., Dobrowolski D., Gabryel B., Małecki A., Tarnawska D., Jurewicz A., and Logiewa-Toborek J. [Culture of the corneal epithelium-comparison of the mitotic potential of Limbal cells from living and cadaveric donors]. Klin. Oczna. 106 (2004) 737-742
168. Xia L.Q. [Air-liquid interface cultivation of human keratinocytes]. Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 11 (1989) 430-432
169. Yang X., Moldovan N.I., Zhao Q., Mi S., Zhou Z., Chen D., Gao Z., Tong D., and Dou Z. Reconstruction of damaged cornea by autologous transplantation of epidermal adult stem cells. Mol. Vis. 14 (2008) 1064-1070
170. Yao Y.F., Zhang B., Zhou P., and Jiang J.K. Autologous limbal grafting combined with deep lamellar keratoplasty in unilateral eye with severe chemical or thermal burn at late stage. Ophthalmology 109 (2002) 2011-2017
171. Yeh H.J., Yao C.L., Chen H.I., Cheng H.C., and Hwang S.M. Cryopreservation of human limbal stem cells ex vivo expanded on amniotic membrane. Cornea 27 (2008) 327-333
172. Yew D.T., Sha O., Li W.W., Lam T.T., and Lorke D.E. Proliferation and apoptosis in the epithelium of the developing human cornea and conjunctiva. Life Sci. 68 (2001) 2987-3003
173. Zhou M., Li X.M., and Lavker R.M. Transcriptional profiling of enriched populations of stem cells versus transient amplifying cells. A comparison of limbal and corneal epithelial basal cells. J. Biol. Chem. 281 (2006) 19600-19609
174. Zhu X., Beuerman R.W., Chan-Park M.B., Cheng Z., Ang L.P., and Tan D.T. Enhancement of the mechanical and biological properties of a biomembrane for tissue engineering the ocular surface. Ann. Acad. Med. Singapore 35 (2006) 210-214