Epithelial proliferative potential of organ cultured corneoscleral rims; implications for allo-limbal transplantation and eye banking

British Journal of Ophthalmology

Volumn 90, Issue 1, 2006, Pages 55-58

  Shanmuganathan, V A ^b   Rotchford, A P ^b   Tullo, A B ^c   Joseph, A ^b   Zambrano, I ^c   Dua, Harminder S ^a,b  

^a NOTTINGHAM UNIVERSITY HOSPITALS NHS TRUST   (United Kingdom)

^b UNIVERSITY OF NOTTINGHAM   (United Kingdom)

^c MANCHESTER ROYAL EYE HOSPITAL   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CELL PROLIFERATION; CONTROLLED STUDY; CORNEA EPITHELIUM; CORNEA LIMBUS; CORNEA TRANSPLANTATION; ENUCLEATION; EXPLANT; EYEBANK; HUMAN; HUMAN CELL; HUMAN TISSUE; IN VITRO STUDY; ORGAN CULTURE; PENETRATING KERATOPLASTY; PRIORITY JOURNAL;

ADULT; AGE FACTORS; AGED; AGED, 80 AND OVER; CELL PROLIFERATION; CORNEAL TRANSPLANTATION; CULTURE MEDIA; EPITHELIUM, CORNEAL; EYE BANKS; HUMANS; LIMBUS CORNEAE; MIDDLE AGED; ORGAN CULTURE TECHNIQUES; STEM CELL TRANSPLANTATION; STEM CELLS; TIME FACTORS; TISSUE AND ORGAN HARVESTING; TISSUE PRESERVATION;

EID: 29744460326     PISSN: 00071161     EISSN: None     Source Type: Journal    
DOI: 10.1136/bjo.2005.071910     Document Type: Article

References (17)

1. Dua HS, Joseph A, Shanmuganathan VA, et al. Stem cell differentiation and the effects of deficiency. Eye 2003;17:877-85.
2. Espana EM, Pascuale MD, Grueterich M, et al. Keratolimbal allograft in corneal reconstruction. Eye 2004;18:406-17.
3. Croasdale CR, Schwartz GS, Malling JV, et al. Keratolimbal allograft: Recommendations for tissue procurement and preparation by the eye banks, and standard surgical technique. Cornea 1999;18:52-8.
4. Armitage WJ, Easty DL. Factors influencing the suitability of organ-cultured corneas for transplantation. Invest Ophthalmol Vis Sci 1997;38:16-24.
5. Mass-Reijs J, Pels E, Tullo AB. Eye banking in Europe 1991-1995. Acta Ophthalmol Scand 1997;75:541-3.
6. James SE, Rowe A, Ilari L, et al. The potential for eye bank limbal rings to generate cultured corneal epithelial allografts. Cornea 2001;20:488-94.
7. Kim Hs, Song XJ, De Paiva CS, et al. Phenotypic characterisation of human corneal epithelial cells expanded ex vivo from limbal explant and single cell cultures. Exp Eye Res 2004;79:41-9.
8. Pellegrini G, Golisano O, Paterna P, et al. Location and clonal analysis of stem cells and their differentiated progeny in the human ocular surface. J Cell Biol 1999;145:769-82.
9. Fuchs E, Tumbar T, Guasch G. Socializing with the neighbors: stem cells and their niche. Cell 2004;116:769-78.
10. Dua HS, Shanmuganathan VA, Powell-Richards AO, et al. Limbal epithelial crypts-a novel anatomical structure and putative stem cell niche. Br J Ophthalmol 2005;89:529-32.
11. Gaudreault M, Carrier P, Larouche K, et al. Influence of Sp1/Sp3 expression on corneal epithelial cells proliferation and differentiation properties in reconstructed tissues. Invest Ophthalmol Vis Sci 2003;44:1447-57.
12. Grueterich M, Espana EM, Tseng SCG. Ex Vivo expansion of limbal stem cells: Amniotic membrane serving as a stem cell niche. Surv Ophthalmol 2003;48:631-46.
13. Nakamura T, Inatomi T, Sotozono C, et al. Transplantation of cultivated autologous oral mucosal epithelial cells in patients with severe ocular surface disorders. Br J Ophthalmol 2004;88:1280-4.
14. Grueterich M, Tseng SCG. Human limbal progenitor cells expanded on intact amniotic membrane ex-vivo. Arch Ophthalmol 2002;120:783-90.
15. Tsubota K, Satake Y, Kaido M, et al. Treatment of severe ocular-surface disorders with corneal epithelium stem-cell transplantation. N Engl J Med 1999;340:1697-703.
16. Ilari L, Daya SM. Long term outcomes of keratolimbal allograft for the treatment of severe ocular surface disorders. Ophthalmology 2002;109:1278-84.
17. Ardjomand N, Berghold A, Reich ME. Loss of corneal Langerhans cells during storage in organ culture medium, Optisol and McCarey-Kaufman medium. Eye 1998;12:134-8.