Rapid preparation of genomic DNA from Monosiga brevicollis and other choanoflagellates

Cold Spring Harbor Protocols

Volumn 4, Issue 2, 2009, Pages

  King, Nicole ^a,b   Young, Susan L ^a   Abedin, Monika ^a   Carr, Martin ^c   Leadbeater, Barry S C ^d  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b CANADIAN INSTITUTE FOR ADVANCED RESEARCH   (Canada)

^c UNIVERSITY OF YORK   (United Kingdom)

^d UNIVERSITY OF BIRMINGHAM   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

GENOMIC DNA; REAGENT;

ARTICLE; CELL CULTURE EQUIPMENT; CHOANOFLAGELLATE; CULTURE MEDIUM; CULTURE TECHNIQUE; DNA ISOLATION; FLAGELLATE; MONOSIGA BREVICOLLIS; NONHUMAN; POLYMERASE CHAIN REACTION; SOUTHERN BLOTTING;

CHOANOFLAGELLIDA; MONOSIGA BREVICOLLIS;

EID: 65749114060     PISSN: None     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot5152     Document Type: Article

References (7)

1. King, N., Young, S.L., Abedin, M., Carr, M., and Leadbeater, B.S.C. 2009a. Preparation of high-molecular-weight genomic DNA from Monosiga brevicollis and other choanoflagellates. Cold Spring Harb. Protoc. (this issue). doi: 10.1101/pdb.prot5153.
2. King, N., Young, S.L., Abedin, M., Carr, M., and Leadbeater, B.S.C. 2009b. Separation of choanoflagellate and bacterial genomic DNA. Cold Spring Harb. Protoc. (this issue). doi: 10.1101/pdb.prot5154.
3. King, N., Young, S.L., Abedin, M., Carr, M., and Leadbeater, B.S.C. 2009c. Preparation of total RNA from Monosiga brevicollis and other choanoflagellates. Cold Spring Harb. Protoc. (this issue). doi: 10.1101/pdb.prot5151.
4. King, N., Young, S.L., Abedin, M., Carr, M., and Leadbeater, B.S.C. 2009d. The choanoflagellates: Heterotrophic nanoflagellates and sister group of the metazoa. Cold Spring Harb. Protoc. (this issue). doi: 10.1101/pdb.emo116.
5. King, N., Young, S.L., Abedin, M., Carr, M., and Leadbeater, B.S.C. 2009e. Isolation of single choanoflagellate cells from field samples and establishment of clonal cultures. Cold Spring Harb. Protoc. (this issue). doi: 10.1101/pdb.prot5147.
6. King, N., Young, S.L., Abedin, M., Carr, M., and Leadbeater, B.S.C. 2009f. Starting and maintaining Monosiga brevicollis cultures. Cold Spring Harb. Protoc. (this issue). doi: 10.1101/pdb.prot5148.
7. Leadbeater, B.S.C., Hassan, R., Nelson, M., Carr, M., and Baldauf, S.L. 2008. A new genus, Helgoeca gen. nov. for a nudiform choanoflagellate. Eur. J. Protistol. 44: 227-237.