Isolation of single choanoflagellate cells from field samples and establishment of clonal cultures

Cold Spring Harbor Protocols

Volumn 4, Issue 2, 2009, Pages

  King, Nicole ^a,b   Young, S L ^a   Abedin, Monika ^a   Carr, Martin ^c   Leadbeater, Barry S C ^d  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b CANADIAN INSTITUTE FOR ADVANCED RESEARCH   (Canada)

^c UNIVERSITY OF YORK   (United Kingdom)

^d UNIVERSITY OF BIRMINGHAM   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CELL CLONING; CELL CULTURE; CELL ISOLATION; CHOANOFLAGELLATE; DILUTION; FLAGELLATE; ISOLATION PROCEDURE; MICROPIPETTE; NONHUMAN;

EID: 65749113346     PISSN: None     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot5147     Document Type: Article

References (2)

1. King, N., Young, S.L., Abedin, M., Carr, M., and Leadbeater, B.S.C. 2009. The choanoflagellates: Heterotrophic nanoflagellates and sister group of the metazoa. Cold Spring Harb. Protoc. (this issue). doi: 10.1101/pdb.emo116.
2. Pratt, J.M. 1984. Coupled transcription-translation in prokaryotic cell-free systems. In Transcription and translation: A practical approach (eds. B.D. Hames and S.J. Higgins), pp. 179-209. IRL, Oxford, UK, New York.