Preparation of high-molecular-weight genomic DNA from Monosiga brevicollis and other choanoflagellates

Cold Spring Harbor Protocols

Volumn 4, Issue 2, 2009, Pages

  King, Nicole ^a,b   Young, Susan L ^a   Abedin, Monika ^a   Carr, Martin ^c   Leadbeater, Barry S C ^d  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b CANADIAN INSTITUTE FOR ADVANCED RESEARCH   (Canada)

^c UNIVERSITY OF YORK   (United Kingdom)

^d UNIVERSITY OF BIRMINGHAM   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

GENOMIC DNA; REAGENT;

ARTICLE; CELL CULTURE EQUIPMENT; CHOANOFLAGELLATE; CULTURE TECHNIQUE; DNA ISOLATION; FLAGELLATE; MOLECULAR WEIGHT; MONOSIGA BREVICOLLIS; NONHUMAN; POLYMERASE CHAIN REACTION; SOUTHERN BLOTTING;

CHOANOFLAGELLIDA; MONOSIGA BREVICOLLIS;

EID: 65749087436     PISSN: None     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot5153     Document Type: Article

References (6)

1. King, N., Westbrook, M.J., Young, S.L., Kuo, A., Abedin, M., Chapman, J., Fairclough, S., Hellsten, U., Isogai, Y., Letunic, I., et al. 2008. The genome of the choanoflagellate Monosiga brevicollis and the origin of the metazoans. Nature 451: 783-788.
2. King, N., Young, S.L., Abedin, M., Carr, M., and Leadbeater, B.S.C. 2009a. Separation of choanoflagellate and bacterial genomic DNA. Cold Spring Harb. Protoc. (this issue). doi: 10.1101/pdb.prot5154.
3. King, N., Young, S.L., Abedin, M., Carr, M., and Leadbeater, B.S.C. 2009b. Rapid preparation of genomic DNA from Monosiga brevicollis and other choanoflagellates. Cold Spring Harb. Protoc. (this issue). doi: 10.1101/pdb.prot5152.
4. King, N., Young, S.L., Abedin, M., Carr, M., and Leadbeater, B.S.C. 2009c. Preparation of total RNA from Monosiga brevicollis and other choanoflagellates. Cold Spring Harb. Protoc. (this issue). doi: 10.1101/pdb.prot5151.
5. King, N., Young, S.L., Abedin, M., Carr, M., and Leadbeater, B.S.C. 2009d. The choanoflagellates: Heterotrophic nanoflagellates and sister group of the metazoa. Cold Spring Harb. Protoc. (this issue). doi: 10.1101/pdb.emo116.
6. King, N., Young, S.L., Abedin, M., Carr, M., and Leadbeater, B.S.C. 2009e. Starting and maintaining Monosiga brevicollis cultures. Cold Spring Harb. Protoc. (this issue). doi: 10.1101/pdb.prot5148.