Separation of choanoflagellate and bacterial genomic DNA

Cold Spring Harbor Protocols

Volumn 4, Issue 2, 2009, Pages

  King, Nicole ^a,b   Young, Susan L ^a   Abedin, Monika ^a   Carr, Martin ^c   Leadbeater, Barry S C ^d  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b CANADIAN INSTITUTE FOR ADVANCED RESEARCH   (Canada)

^c UNIVERSITY OF YORK   (United Kingdom)

^d UNIVERSITY OF BIRMINGHAM   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; CYTOSINE; GENOMIC DNA; GUANINE; REAGENT;

ARTICLE; CELL CULTURE EQUIPMENT; CENTRIFUGATION; CHOANOFLAGELLATE; DNA CONTENT; FLAGELLATE; NONHUMAN; PRECIPITATION; SEPARATION TECHNIQUE;

BACTERIA (MICROORGANISMS);

EID: 65749097615     PISSN: None     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot5154     Document Type: Article

References (3)

1. Flamm, W.G., Birnstiel, M.L., and Walker, P.M.B. 1972. Isopycnic centrifugation of DNA: Methods and applications. In Subcellular components: Preparation and fractionation (ed. G.D. Birnie), pp. 279-310. Butterworth, London, and University Park Press, Baltimore, MD.
2. King, N., Young, S.L., Abedin, M., Carr, M., and Leadbeater, B.S.C. 2009a. The choanoflagellates: Heterotrophic nanoflagellates and sister group of the metazoa. Cold Spring Harb. Protoc. (this issue). doi: 10.1101/pdb.emo116.
3. King, N., Young, S.L., Abedin, M., Carr, M., and Leadbeater, B.S.C. 2009b. Preparation of high-molecular-weight genomic DNA from Monosiga brevicollis and other choanoflagellates. Cold Spring Harb. Protoc. (this issue). doi: 10.1101/pdb.prot5153.