Preparation of total RNA from Monosiga brevicollis and other choanoflagellates

Cold Spring Harbor Protocols

Volumn 4, Issue 2, 2009, Pages

  King, Nicole ^a,b   Young, Susan L ^a   Abedin, Monika ^a   Carr, Martin ^c   Leadbeater, Barry S C ^d  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b CANADIAN INSTITUTE FOR ADVANCED RESEARCH   (Canada)

^c UNIVERSITY OF YORK   (United Kingdom)

^d UNIVERSITY OF BIRMINGHAM   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

REAGENT; RNA;

ARTICLE; CELL CULTURE EQUIPMENT; CENTRIFUGATION; CHOANOFLAGELLATE; FLAGELLATE; HOMOGENATE; MONOSIGA BREVICOLLIS; NONHUMAN; REAL TIME POLYMERASE CHAIN REACTION; RNA DEGRADATION; SPECTROPHOTOMETER; TEMPERATURE;

CHOANOFLAGELLIDA; MONOSIGA BREVICOLLIS;

EID: 65749100362     PISSN: None     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot5151     Document Type: Article

References (5)

1. King, N., Young, S.L., Abedin, M., Carr, M., and Leadbeater, B.S.C. 2009a. The choanoflagellates: Heterotrophic nanoflagellates and sister group of the metazoa. Cold Spring Harb. Protoc. (this issue). doi: 10.1101/pdb.emo116.
2. King, N., Young, S.L., Abedin, M., Carr, M., and Leadbeater, B.S.C. 2009b. Rapid preparation of genomic DNA from Monosiga brevicollis and other choanoflagellates. Cold Spring Harb. Protoc. (this issue). doi: 10.1101/pdb.prot5152.
3. King, N., Young, S.L., Abedin, M., Carr, M., and Leadbeater, B.S.C. 2009c. Preparation of high-molecular-weight genomic DNA from Monosiga brevicollis and other choanoflagellates. Cold Spring Harb. Protoc. (this issue). doi: 10.1101/pdb.prot5153.
4. King, N., Young, S.L., Abedin, M., Carr, M., and Leadbeater, B.S.C. 2009d. Separation of choanoflagellate and bacterial genomic DNA. Cold Spring Harb. Protoc. (this issue). doi: 10.1101/pdb.prot5154.
5. King, N., Young, S.L., Abedin, M., Carr, M., and Leadbeater, B.S.C. 2009e. Starting and maintaining Monosiga brevicollis cultures. Cold Spring Harb. Protoc. (this issue). doi: 10.1101/pdb.prot5148.