A surface display yeast two-hybrid screening system for high-throughput protein interactome mapping

Analytical Biochemistry

Volumn 390, Issue 1, 2009, Pages 29-37

  Chen, Jun ^a   Zhou, Jianhong ^a   Sanders, Claire K ^a   Nolan, John P ^b   Cai, Hong ^a  

^a LOS ALAMOS NATIONAL LABORATORY   (United States)

^b LA JOLLA BIOENGINEERING INSTITUTE   (United States)

Author keywords

cDNA library screening; High throughput; Surface display; Y2H; yEGFP

Indexed keywords

ANTIGEN-ANTIBODY REACTIONS; DNA SEQUENCES; GENE ENCODING; LIQUIDS; MAGNETIC SEPARATION; MAPPING; NUTRIENTS; YEAST;

CDNA LIBRARIES; HIGH THROUGHPUT; HIGH THROUGHPUT SCREENING; PROTEIN INTERACTION NETWORKS; PROTEIN-PROTEIN INTERACTIONS; SURFACE DISPLAYS; YEAST ENHANCED GREEN FLUORESCENT PROTEIN (YEGFP); YEGFP;

PROTEINS;

GREEN FLUORESCENT PROTEIN;

ARTICLE; DNA LIBRARY; DNA SEQUENCE; HEMAGGLUTINATION; HIGH THROUGHPUT SCREENING; LIQUID CULTURE; MAGNETIC SEPARATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN INTERACTION; QUANTITATIVE ANALYSIS; YEAST;

EID: 65649100527     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2009.03.013     Document Type: Article

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