"Enzyme Test Bench," a high-throughput enzyme characterization technique including the long-term stability

Biotechnology and Bioengineering

Volumn 103, Issue 2, 2009, Pages 305-322

  Rachinskiy, Kirill ^a   Schultze, Hergen ^b   Boy, Matthias ^b   Bornscheuer, Uwe ^c   Büchs, Jochen ^a  

^a RWTH AACHEN UNIVERSITY   (Germany)

^b BASF SE   (Germany)

^c Institute of Biochemistry   (Germany)

Author keywords

Esterase; Experimental design; Fluorescent assay; High throughput; Long term stability; Microtiter plate; Modeling; Online monitoring; Parameter estimation; pH shift

Indexed keywords

ESTERASE; EXPERIMENTAL DESIGN; FLUORESCENT ASSAY; HIGH THROUGHPUT; LONG TERM STABILITY; MICROTITER PLATE; MODELING; ONLINE MONITORING;

CATALYSTS; ENZYMES; ESTERS; EXPERIMENTS; FLUORESCENCE; INSTRUMENTS; METAL ANALYSIS; MONITORING; OPTIMIZATION; PARAMETER ESTIMATION; PARTIAL DISCHARGES; STABILITY; STATISTICS; TEMPERATURE CONTROL; TESTING; THROUGHPUT;

ENZYME ACTIVITY;

ESTERASE;

ANALYTIC METHOD; ARTICLE; CALCULATION; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME ASSAY; ENZYME INACTIVATION; ENZYME STABILITY; ENZYME TEST BENCH; EXPERIMENTAL DESIGN; FLUORESCENCE; HYDROLYSIS; MATHEMATICAL MODEL; MICROTITER PLATE ASSAY; PH; REACTOR; SCREENING; TEMPERATURE ACCLIMATIZATION; TEMPERATURE DEPENDENCE; THERMAL CONDUCTIVITY; AUTOMATION; CHEMISTRY; METABOLISM; TEMPERATURE; THEORETICAL MODEL;

AUTOMATION; ENZYME STABILITY; ESTERASES; MODELS, THEORETICAL; TEMPERATURE;

EID: 65549139244     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22242     Document Type: Article

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