Functional characterization and substrate specificity of spinosyn rhamnosyltransferase by in Vitro reconstitution of spinosyn biosynthetic enzymes

Journal of Biological Chemistry

Volumn 284, Issue 11, 2009, Pages 7352-7363

  Chen, Yi Lin ^a   Chen, Yi Hsine ^a   Lin, Yu Chin ^a   Tsai, Kuo Chung ^a   Chiu, Hsien Tai ^a  

^a NATIONAL CHIAO TUNG UNIVERSITY   (Taiwan)

Author keywords

[No Author keywords available]

Indexed keywords

BIOLOGICAL ACTIVITIES; BIOSYNTHETIC ENZYMES; CATALYTIC MECHANISMS; CELL WALL BIOSYNTHESIS; COMBINATORIAL TOOLS; FUNCTIONAL CHARACTERIZATION; GLYCOSYLATED; IN-SITU; IN-VITRO; INSECTICIDAL ACTIVITY; L-RHAMNOSE; POLYKETIDE; PURIFIED ENZYME; SITE DIRECTED MUTAGENESIS; STRUCTURE-ACTIVITY; SUBSTRATE SPECIFICITY; UNIQUE SEQUENCE;

BIOCHEMICAL ENGINEERING; BIOCHEMISTRY; BIOSYNTHESIS; CATALYSTS; ENZYMES; ESCHERICHIA COLI; MOLECULAR BIOLOGY; MOLECULAR RECOGNITION; SUBSTRATES; SUGAR (SUCROSE); SUGARS;

ENZYME INHIBITION;

4 KETO 6 DEOXYGLUCOSE EPIMERASE; 4 KETORHAMNOSE REDUCTASE; BACTERIAL ENZYME; GLUTAMATE DEHYDROGENASE; OXIDOREDUCTASE; SPINOSYN RHAMNOSYLTRANSFERASE; UNCLASSIFIED DRUG; BACTERIAL PROTEIN; GLYCOSYLTRANSFERASE; INSECTICIDE; MACROLIDE; NUCLEOSIDE DIPHOSPHATE SUGAR; PYRIMIDINE NUCLEOTIDE; RECOMBINANT PROTEIN; THYMIDINE DIPHOSPHATE RHAMNOSE;

ACTINOBACTERIA; ARTICLE; BIOSYNTHESIS; CATALYSIS; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME INHIBITION; ENZYME KINETICS; ENZYME SPECIFICITY; ENZYME STRUCTURE; IN VITRO STUDY; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; SACCHAROPOLYSPORA SPINOSA; SITE DIRECTED MUTAGENESIS; CHEMISTRY; ENZYMOLOGY; GENETICS; METABOLISM; PHYSIOLOGY; PROTEIN TERTIARY STRUCTURE; SACCHAROPOLYSPORA; STRUCTURE ACTIVITY RELATION;

ESCHERICHIA COLI; SACCHAROPOLYSPORA SPINOSA;

BACTERIAL PROTEINS; CATALYSIS; HEXOSYLTRANSFERASES; INSECTICIDES; MACROLIDES; NUCLEOSIDE DIPHOSPHATE SUGARS; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; SACCHAROPOLYSPORA; STRUCTURE-ACTIVITY RELATIONSHIP; SUBSTRATE SPECIFICITY; THYMINE NUCLEOTIDES;

EID: 65449167261     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M808441200     Document Type: Article

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