Identification and characterization of GDP-D-mannose 4, 6-dehydratase and GDP-L-fucose synthetase in a GDP-L-fucose biosynthetic gene cluster from Helicobacter pylori

Biochemical and Biophysical Research Communications

Volumn 285, Issue 2, 2001, Pages 364-371

  Wu, Bingyuan ^a   Zhang, Yingxin ^a   Wang, Peng George ^a  

^a WAYNE STATE UNIVERSITY   (United States)

Author keywords

GDP 4 keto 6 deoxy D mannose; GDP D mannose; GDP D mannose 4, 6 dehydratase; GDP L fucose; GDP L fucose synthetase; Helicobacter pylori

Indexed keywords

GUANOSINE DIPHOSPHATE FUCOSE; GUANOSINE DIPHOSPHATE FUCOSE SYNTHETASE; GUANOSINE DIPHOSPHATE MANNOSE; GUANOSINE DIPHOSPHATE MANNOSE 4,6 DEHYDRATASE; HYDROLYASE; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; RECOMBINANT ENZYME; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; SYNTHETASE; TETRAMER; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL GENE; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE CLUSTER; GENE OVEREXPRESSION; HELICOBACTER PYLORI; MOLECULAR CLONING; NONHUMAN; OPEN READING FRAME; PH; PHYLOGENY; PRIORITY JOURNAL;

ESCHERICHIA COLI; HELICOBACTER PYLORI;

EID: 0034806401     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1006/bbrc.2001.5137     Document Type: Article

References (34)

1. GDP-L-fucose synthetase from Escherichia coli: Sructure of a unique member of the short-chain dehydrogenase/reductase family that catalyzes two distinct reactions at the same active site
2. Expression of the human cell surface glycoconjugates Lewis x and Lewis y by Helicobacter pylori isolates is related to cagA status
3. Lipopolysaccharides of Helicobacter pylori strains P466 and MO19: Structures of the O antigen and core oligosaccharide regions
4. Simultaneous expression of type 1 and type 2 Lewis blood group antigens by Helicobacter pylori lipopolysaccharides. Molecular mimicry between H. pylori lipopolysaccharides and human gastric epithelial cell surface glycoforms
5. Formation of guanosine diphosphate L-fucose from guanosine diphosphate D-mannose
6. Studies on the biosynthesis of guanosine diphosphate L-fucose
7. The metabolism of 6-deoxy-hexoses in bacterial and animal cells
8. Structural and kinetic analysis of Escherichia coli GDP-D-mannose 4, 6 dehydratase provides insights into the enzyme’s catalytic mechanism and regulation by GDP-L-fucose
9. Molecular cloning of human GDP-D-mannose 4, 6-dehydratase and reconstitution of GDP-L-fucose biosynthesis in vitro
10. An epimerase-reductase in L-fucose synthesis
11. Stereochemical course and steady state mechanism of the reaction catalyzed by the GDP-L-fucose synthetase from Escherichia coli
12. Probing the catalytic mechanism of GDP-4-keto-6-deoxy-D-mannose epimerase/reductase by kinetic and crystallographic characterization of site-specific mutants
13. Purification and characterization of GDP-D-mannose 4, 6-dehydratase from porcine thyroid
14. Evidence that the enzyme catalyzing the conversion of guanosine diphosphate D-mannose to a 4-keto sugar nucleotide intermediate requires nicotinamide adenine dinucleotide phosphate
15. Expression, purification and characterization of GDP-D-mannose 4, 6-dehydratase from Escherichia coli
16. The MUR1 gene of Arabidopsis thaliana encodes an isoform of GDP-D-mannose-4, 6-dehydratase, catalyzing the first step in the de novo synthesis of GDP-L-fucose
17. Synthesis of GDP-L-fucose by the human FX protein
18. Identification of the fucose synthetase gene in the colanic acid gene cluster of Escherichia coli K-12
19. A bifunctional epimerase-reductase acts downstream of the MUR1 gene product and completes the de novo synthesis of GDP-L-fucose in Arabidopsis
20. GDP-4-keto-6-deoxy-D-mannose epimerase/reductase from Escherichia coli, a key enzyme in the biosynthesis of GDP-L-fucose, displays the structural characteristics of the RED protein homology superfamily
21. Gapped BLAST and PSI-BLAST: A new generation of protein database search programs
22. Multiple sequence alignment with hierarchical clustering
23. Structural and enzymatic characterization of human recombinant GDP-D-mannose-4, 6-dehydratase
24. Short-chain dehydrogenases/reductases (SDR)
25. Structural comparisons lead to the definition of a new superfamily of NAD(P) (H)-accepting oxidoreductases: The single-domain reductases/epimerases/dehydrogenases (the ’RED’ family)
26. GDP-4-keto-6-deoxy-D-mannose epimerase/reductase from Escherichia coli, a key enzyme in the biosynthesis of GDP-L-fucose, displays the structural characteristics of the RED protein homology superfamily
27. Bacterial polysaccharide synthesis and gene nomenclature
28. The JUMPstart sequence: A 39 bp element common to several polysaccharide gene clusters
29. Variation of the rfb gene clusters in Salmonella enterica
30. The colanic acid gene cluster of Salmonella enterica has a complex history
31. Organization of the Escherichia coli K-12 gene cluster responsible for production of the extracellular polysaccharide colanic acid
32. Characterization of the O-antigen gene clusters of Yersinia pseudotuberculosis and the cryptic O-antigen gene cluster of Yersinia pestis shows that the plague bacillus is most closely related to and has evolved from Y. pseudotuberculosis serotype O:1b
33. Sequence and analysis of the O antigen gene (rfb) cluster of Escherichia coli O111
34. Analysis of the genes responsible for the O-antigen synthesis in enterohaemorrhagic Escherichia coli O157