Structure of the UDP-glucosyltransferase GtfB that modifies the heptapeptide aglycone in the biosynthesis of vancomycin group antibiotics

Structure

Volumn 9, Issue 7, 2001, Pages 547-557

  Mulichak, Anne M ^a   Losey, Heather C ^b   Walsh, Christopher T ^b   Garavito, R Michael ^a  

^a Michigan State University   (United States)

^b HARVARD MEDICAL SCHOOL   (United States)

Author keywords

Antibiotic biosynthesis; Family 1 glycosyltransferases, UDP glucose; Glycosylation; Rossmann fold; Vancomycin; X ray crystal structure

Indexed keywords

ANTIBIOTIC AGENT; CHLOROEREMOMYCIN; DISACCHARIDE; GLUCOSYLTRANSFERASE; HEPTAPEPTIDE; L 4 EPI VANCOSAMINE 1,2 GLUCOSE; L VANCOSAMINE 1,2 GLUCOSE; MUTANT PROTEIN; PROTEIN D13A; PROTEIN D332A; PROTEIN GTFB; PROTEIN H125A; UNCLASSIFIED DRUG; URIDINE DIPHOSPHATE GLUCOSE; VANCOMYCIN;

AMINO TERMINAL SEQUENCE; ANTIBIOTIC BIOSYNTHESIS; ANTIBIOTIC RESISTANCE; ARTICLE; BINDING SITE; CARBOXY TERMINAL SEQUENCE; DRUG EFFICACY; DRUG STRUCTURE; ENZYME STRUCTURE; ESCHERICHIA COLI; GLYCOSYLATION; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN PROCESSING; PROTEIN PURIFICATION; STEREOCHEMISTRY; X RAY CRYSTALLOGRAPHY;

AMINO ACID SEQUENCE; ANTI-BACTERIAL AGENTS; BINDING SITES; CATALYSIS; CRYSTALLOGRAPHY, X-RAY; GLUCOSYLTRANSFERASES; GLYCOSYLATION; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PEPTIDES; PROTEIN CONFORMATION; SEQUENCE HOMOLOGY, AMINO ACID; VANCOMYCIN;

ESCHERICHIA COLI; NEGIBACTERIA; T4 PHAGE;

EID: 0034938546     PISSN: 09692126     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0969-2126(01)00616-5     Document Type: Article

References (40)

1. Medical consequences of antibiotic use in agriculture
2. The specter of glycopeptide resistance: Current trends and future considerations
3. Reductive alkylation of glycopeptide antibiotics: Synthesis and antibacterial activity
4. Novel glycopeptide antibiotics: N-alkylated derivatives active against vancomycin-resistant enterococci
5. Vancomycin derivatives that inhibit peptidoglycan biosynthesis without binding D-Ala-D-Ala
6. Sequencing and analysis of genes involved in the biosynthesis of a vancomycin group antibiotic
7. The role of the hydrophobic substituents in the biological activity of glycopeptide antibiotics
8. Glycopeptide antibiotic activity and the possible role of dimerization: A model for biological signaling
9. Binding of glycopeptide antibiotics to a model of a vancomycin-resistant bacterium
10. Overproduction and characterization of the erythromycin C-12 hydroxylase, EryK
11. Analysis of seven genes from the eryAI-eryK region of the erythromycin biosynthetic gene cluster in Saccharopolyspora erythraea
12. Analysis of eryBI, eryBIII and eryBVII from the erythromycin biosynthetic gene cluster in Saccharopolyspora erythraea
13. Glycosylation of macrolide antibiotics; purification and kinetic studies of a macrolide glycosyltransferase from Streptomyces antibioticus
14. Crystal structure of the DNA modifying enzyme beta-glucosyltransferase in the presence and absence of the substrate uridine diphosphoglucose
15. The 1.9 Å crystal structure of E. coli MurG, a membrane-associated glycosyltransferase involved in peptidoglycan biosynthesis
16. Structure of the nucleotide-diphospho-sugar transferase, SpsA from Bacillus subtilis, in native and nucleotide-complexed forms
17. Crystal structures of the bovine β4galactosyltransferase catalytic domain and its complex with uridine diphosphogalactose
18. X-ray crystal structure of rabbit N-acetylglucosaminyltransferase I: Catalytic mechanism and a new protein superfamily
19. Heparan/chondroitin sulfate biosynthesis. Structure and mechanism of human glucuronyltransferase I
20. Bovine α1,3-galactosyltransferase catalytic domain structure and its relationship with ABO histo-blood group and glycosphingolipid glycosyltransferases
21. Crystal structure of the retaining galactosyltransferase LgtC from Neisseria meningitidis in complex with donor and acceptor sugar analogs
22. T4 phage beta-glucosyltransferase: Substrate binding and proposed catalytic mechanism
23. A classification of nucleotide-disphospho-sugar glycosyltransferases based on amino acid sequence similarities
24. Glycosyltransferase structure and mechanism
25. Beta-glucosyltransferase and phosphorylase reveal their common theme
26. Evolutionary link between glycogen phosphorylase and a DNA modifying enzyme
27. Crystal structure of vancomycin
28. Site-directed mutagenesis by overlap extension using the polymerase chain reaction
29. Production of hybrid glycopeptide antibiotics in vitro and in Streptomyces toyocaensis
30. Tandem action of glycosyltransferases in the maturation of vancomycin and teicoplanin aglycones: Novel glycopeptides
31. Automated structure solution for MIR and MAD
32. Joint CCP4 ESF-EACBM Newslett
33. The CCP4 (Collaborative Computational Project Number 4) suite: Programs for protein crystallography
34. Processing of X-ray diffraction data collected in oscillation mode
35. Crystallography and NMR system (CNS): A new software system for macromolecular structure determination
36. Procheck: A program to check the stereochemical quality of protein structures
37. Setor: Hardware lighted three-dimensional solid model representations of macromolecules
38. RASTER3D version 2.0. A program for photorealistic molecular graphics