Ethanol diversely alters palmitate, stearate, and oleate metabolism in the liver and pancreas of rats using the deuterium oxide single tracer

Pancreas

Volumn 38, Issue 2, 2009, Pages

  Boros, Laszlo G ^a,b   Deng, Qinggao ^c   Pandol, Stephen J ^c,d   Tsukamoto, Hidekazu ^c,d   Go, Vay Liang W ^e   Lee, Wai Nang Paul ^a,b,e  

^a HARBOR UCLA MEDICAL CENTER   (United States)

^b UNIVERSITY OF SOUTHERN CALIFORNIA   (United States)

^c UNIVERSITY OF SOUTHERN CALIFORNIA   (United States)

^d VA GREATER LOS ANGELES HEALTHCARE SYSTEM   (United States)

^e UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Deuterium; Fatty acid ratios; Oleic acid; Palmitic acid; Stearic acid

Indexed keywords

ALCOHOL; DEUTERIUM OXIDE; FATTY ACID; OLEIC ACID; PALMITIC ACID; STABLE ISOTOPE; STEARIC ACID; TRACER; DIAGNOSTIC AGENT; STEARIC ACID DERIVATIVE;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BIOACCUMULATION; CONTROLLED STUDY; FATTY ACID METABOLISM; FATTY ACID SYNTHESIS; FATTY ACID TRANSPORT; ISOTOPE LABELING; LIPOGENESIS; LIVER; LONG TERM EXPOSURE; MALE; MASS FRAGMENTOGRAPHY; NONHUMAN; PANCREAS; PANCREAS FUNCTION; PRIORITY JOURNAL; RAT; WISTAR RAT; ANIMAL; CALORIC INTAKE; DRUG EFFECT; METABOLISM;

ANIMALS; DEUTERIUM OXIDE; ENERGY INTAKE; ETHANOL; LIVER; MALE; OLEIC ACID; PALMITIC ACID; PANCREAS; RATS; RATS, WISTAR; STEARIC ACIDS;

EID: 65349133060     PISSN: 08853177     EISSN: None     Source Type: Journal    
DOI: 10.1097/MPA.0b013e318199fea4     Document Type: Article

References (30)

1. Maguire G, Lee P, Manheim D, et al. SiDMAP: a metabolomic approach to assess the effects of drug candidates on the dynamic properties of biochemical pathways. Expert Opin Drug Discov. 2006; 1:351-359.
2. Maguire G, Lee P, Boros L. Development of tracer-based metabolomics and its implications for the pharmaceutical industry. Int J Pharm Med. 2007;21:217-224.
3. Dunn WB, Ellis DI. Metabolomics: current analytical platforms and methodologies. Trends Anal Chem TRAC. 2005;24:285-294.
4. Fiehn O. Metabolomics: the link between genotypes and phenotypes. Plant Mol Biol. 2002;48:155-171.
5. Goodacre R, Vaidyanathan S, Dunn WB, et al. Metabolomics by numbers: acquiring and understanding global metabolite data. Trends Biotech. 2004;22:245-252.
6. Boros LG, Cascante M, Lee WN. Metabolic profiling of cell growth and death in cancer: applications in drug discovery. Drug Discov Today. 2002;7(6):364-372.
7. Buchanan TA, Xiang A, Kjos SL, et al. Gestational diabetes: antepartum characteristics that predict postpartum glucose intolerance and type 2 diabetes in Latino women. Diabetes. 1998;47(8):1302-1310.
8. Axelson M, Cronholm T, Sahlberg BL, et al. Changes in the metabolic profile of steroids in urine during ethanol metabolism in man. J Steroid Biochem. 1981;14:155-159.
9. Weiner H, Coker FG, Vrbanac JJ. Application of metabolic profiling to study the effects of ethanol on metabolism in rats. Alcohol. 1984;1:105-109.
10. Patton GM, Cann S, Brunengraber H, et al. Separation of methyl esters of fatty acids by gas chromatography on capillary columns, including the separation of deuterated from nondeuterated fatty acids. Methods Enzymol. 1981;72:8-20.
11. Tsukamoto H, French SW, Benson N, et al. Severe and progressive steatosis and focal necrosis in rat liver induced by continuous intragastric infusion of ethanol and low fat diet. Hepatology. 1985;5: 224-232.
12. Tsukamoto H, Reidelberger RD, French SW, et al. Long-term cannulation model for blood sampling and intragastric infusion in the rat. Am J Physiol. 1984;247:R595-R599.
13. Bassilian S, Ahmed S, Lim SK, et al. Loss of regulation of lipogenesis in the Zucker diabetic rat. II. Changes in stearate and oleate synthesis. Am J Physiol Endocrinol Metab. 2002;282: E507-E513.
14. Lee WN, Bassilian S, Lim S, et al. Loss of regulation of lipogenesis in the Zucker diabetic (ZDF) rat. Am J Physiol Endocrinol Metab. 2000;279:E425-E432.
15. Aije HO, Connor MJ, Lee WNP, et al. In vivo study of the biosynthesis of long chain fatty acids (LCFA) using deuterated water (D2O). Am J Physiol. 1995;269:E247-E252.
16. Wadke M, Brunengraber H, Lowenstein JM, et al. Fatty acid synthesis by liver perfused with deuterated and tritiated water. Biochemistry. 1973;12:2619-2624.
17. Lee WNP, Byerley LO, Bergner EA, et al. Mass isotopomer analysis: theoretical and practical considerations. Biol Mass Spectrom. 1991; 20:451-458.
18. Lee WNP. Stable isotope and mass isotopomer study of fatty acid and cholesterol synthesis: a review of the MIDA approach. In: Heber D, Kritchevsy D, eds. Dietary Fats, Lipids, Hormones and Tumorogenesis: New Horizons in Basic Research. London, England: Plenum Publishers; 1996:95-114.
19. Crabb DW. Alcohol deranges hepatic lipid metabolism via altered transcriptional regulation. Trans Am Clin Climatol Assoc. 2004;115: 273-287.
20. Crabb DW, Liangpunsakul S. Alcohol and lipid metabolism. J Gastroenterol Hepatol. 2006;21(suppl 3):S56-S60.
21. Lieber CS. Alcoholic fatty liver: its pathogenesis and mechanism of progression to inflammation and fibrosis. Alcohol. 2004;34:9-19.
22. Mutch DM, Fauconnot L, Grigorov M, et al. Putting the "Ome" in lipid metabolism. Biotechnol Annu Rev. 2006;12:67-84.
23. Bunout D. Nutritional and metabolic effects of alcoholism: their relationship with alcoholic liver disease. Nutrition. 1999;15: 583-589.
24. Pawlosky RJ, Salem N Jr. Alcohol consumption in rhesus monkeysdepletes tissues of polyunsaturated fatty acids and alters essential fatty acid metabolism. Alcohol Clin Exp Res. 1999;23:311-317.
25. Tsukamoto H, Mkrtchyan H, Dynnyk A. Intragastric ethanol infusion model in rodents. In: Nagy LE, ed. Alcohol: Methods and Protocols. Totowa, NJ: Humana Press; 2008:33-48.
26. French SW, Miyamoto K, Tsukamoto H. Ethanol-induced hepatic fibrosis in the rat: role of the amount of dietary fat. Alcohol Clin Exp Res. 1986;10:13S-19S.
27. Nakamura MT, Tang AB, Villanueva J, et al. Selective reduction of delta 6 and delta 5 desaturase activities but not delta 9 desaturase in micropigs chronically fed ethanol. J Clin Invest. 1994;93:450-454.
28. Werner J, Laposata M, Fernández-del Castillo C, et al. Pancreatic injury in rats induced by fatty acid ethyl ester, a nonoxidative metabolite of alcohol. Gastroenterology. 1997;113:286-294.
29. Haber PS, Apte MV, Moran C, et al. Non-oxidative metabolism of ethanol by rat pancreatic acini. Pancreatology. 2004;4:82-89.
30. Apte MV, Norton ID, Wilson JS. Ethanol induced acinar cell injury. Alcohol Alcohol Suppl. 1994;2:365-368.