Probing the role of the proximal heme ligand in cytochrome P450cam by recombinant incorporation of selenocysteine

Proceedings of the National Academy of Sciences of the United States of America

Volumn 106, Issue 14, 2009, Pages 5481-5486

  Aldag, Caroline ^a   Gromov, Igor A ^a   García Rubio, Inés ^a   Von Koenig, Konstanze ^b   Schlichting, Ilme ^b   Jaun, Bernhard ^a   Hilvert, Donald ^a  

^a ETH ZURICH   (Switzerland)

^b MAX PLANCK INSTITUTE FOR MEDICAL RESEARCH   (Germany)

Author keywords

Protein engineering; Selenocysteine insertion sequence element selenoenzyme; Stop codon suppression; X ray crystallography

Indexed keywords

CAMPHOR 5 MONOOXYGENASE; HEME; SELENOCYSTEINE;

AMINO ACID SUBSTITUTION; ARTICLE; CATALYSIS; CODON USAGE; COMPLEX FORMATION; CRYSTAL STRUCTURE; ELECTRON TRANSPORT; ENZYME ACTIVITY; ENZYME STRUCTURE; ENZYME SYNTHESIS; OXIDATION; PRIORITY JOURNAL; PROTEIN ENGINEERING; PROTEIN FUNCTION; X RAY CRYSTALLOGRAPHY;

AMINO ACID SUBSTITUTION; CAMPHOR 5-MONOOXYGENASE; CLONING, MOLECULAR; CRYSTALLOGRAPHY, X-RAY; ELECTRON TRANSPORT; ESCHERICHIA COLI; HEME; KINETICS; LIGANDS; MOLECULAR PROBE TECHNIQUES; OXIDATION-REDUCTION; PROTEIN ENGINEERING; SELENOCYSTEINE;

BACTERIA (MICROORGANISMS);

EID: 65249158572     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.0810503106     Document Type: Article

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