Transepithelial transport characteristics of the antihypertensive peptide, lys-val-leu-pro-val-pro, in human intestinal caco-2 cell monolayers

Bioscience, Biotechnology and Biochemistry

Volumn 73, Issue 2, 2009, Pages 293-298

  Sun, Haiyan ^a   Liu, Dong ^a   Li, Shimin ^a   Qin, Zhenyu ^b  

^a SHENZHEN POLYTECHNIC   (China)

^b UNIVERSITY OF CINCINNATI COLLEGE OF MEDICINE   (United States)

Author keywords

Antihypertensive peptide; Caco 2 cell; High performance liquid chromatography (HPLC); Lys Val Leu Pro Val Pro; Transepithelial transport

Indexed keywords

ANTIHYPERTENSIVE PEPTIDE; CACO-2 CELL; HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC); LYS-VAL-LEU-PRO-VAL-PRO; TRANSEPITHELIAL TRANSPORT;

AMINES; ANIMAL CELL CULTURE; BLOOD PRESSURE; BODY FLUIDS; CHROMATOGRAPHIC ANALYSIS; CHROMATOGRAPHY; HIGH PRESSURE LIQUID CHROMATOGRAPHY; LIQUIDS; SODIUM; SUGAR (SUCROSE);

HIGH PERFORMANCE LIQUID CHROMATOGRAPHY;

RATTUS;

ANTIHYPERTENSIVE AGENT; CARRIER PROTEIN; OLIGOPEPTIDE; PEPTIDE PERMEASE; SODIUM AZIDE;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; CELL STRAIN CACO 2; CHEMISTRY; CYTOLOGY; CYTOSOL; DIFFUSION; DOSE RESPONSE; DRUG EFFECT; EPITHELIUM CELL; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; INCUBATOR; INTESTINE; METABOLISM; REPRODUCIBILITY; TIME;

AMINO ACID SEQUENCE; ANIMALS; ANTIHYPERTENSIVE AGENTS; CACO-2 CELLS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CYTOSOL; DIFFUSION; DOSE-RESPONSE RELATIONSHIP, DRUG; EPITHELIAL CELLS; HUMANS; INCUBATORS; INTESTINES; MEMBRANE TRANSPORT PROTEINS; OLIGOPEPTIDES; REPRODUCIBILITY OF RESULTS; SODIUM AZIDE; TIME FACTORS;

EID: 65249103391     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.80473     Document Type: Article

References (33)

1. Imazu, M., Hypertension and insulin disorders. Curr. Hyper-tens. Rep., 4, 477-482 (2002).
2. Karaki, H., Doi, K., and Sugano, S., Antihypertensive effect of tryptic hydrolyzate of milk casein in spontaneously hypertensive rats. Comp. Biochem. Physiol., 96, 367-371 (1990).
3. Yoshikawa, M., Fujita, H., and Matoba, N., Bioactive peptides derived from food proteins preventing lifestyle-related diseases. Biofactors, 12, 143-146 (2000).
4. Fujita, H., Yokoyama, K., and Yoshikawa, M., Classification and antihypertensive activity of angiotensin I-converting enzyme inhibitory peptides derived from food proteins. J. Food Sci, 65, 564-569 (2000).
5. Arai, S., Osawa, T., and Ohigashi, H., A mainstay of functional food science in Japan: history, present status, and future outlook. Biosci. Biotechnol. Biochem., 65, 1-13 (2001).
6. Pappenheimer, J. R., Dahl, C. E., and Karnovsky, M. L., Intestinal absorption and excretion of octapeptides composed of D-amino acids. Proc. Natl. Acad. Sci. USA, 91, 1942-1945 (1994).
7. Adson, A., Raub, T. J., and Burton, P. S., Quantitative approaches to declineate paracellular diffusion in cultured epithelial cell monolayers. J. Pharm. Sci., 83, 1529-1536 (1994).
8. Shimizu, M., Tsunogai, M., and Arai, S., Transepithelial transport of oligopeptides in the human intestinal cell, Caco-2. Peptides, 18, 681-687 (1997).
9. Sai, M., Kajita, M., and Tamai, I., Adsorptive-mediated endocytosis of a basic peptide in erythrocyte-like Caco-2 cells. Am. J. Physiol., 275, 514-520 (1998).
10. Fei, Y. J., Kanai, Y., and Nussberger, S., Expression cloning of a mammalian proton-coupled oligopeptides transporter. Nature, 368, 563-566 (1994).
11. +/peptide cotransporter. J. Biol. Chem., 270, 6456-6463 (1995).
12. Liu, D., Sun, H. Y., and Zhang, L. J., High-level expression of milk-derived AHP in Escherichia coli and its bioactivity. J. Agric. Food Chem., 55, 5109-5112 (2007).
13. Hidalgo, I. J., Raub, T. J., and Borchardt, R. T., Characterization of the human colon carcinoma cell line (Caco-2) as a model system for intestinal epithelial permeability. Gastroenterology, 96, 736-749 (1989).
14. Artursson, P., Palm, K., and Luthman, K., Caco-2 monolayers in experimental and theoretical predictions of drug transport. Drug Deliv. Rev., 46, 27-43 (2001).
15. Zhu, X. L., Matsui, T., Watanabe, K., Shiraishi, K., Ueki, T., and Matsumoto, K., Simultaneous determination of Angiotensin I-converting enzyme inhibitory peptides in tryptic casein hydrolysate by high-performance liquid chromatography combined with a replicate heart-cut column-switching technique. J. Agric. Food Chem., 55, 8367-8371 (2007).
16. Irvine, J. D., Takahashi, L., and Lockhart, K., MDCK (Madin-Darby canine kidney) cells: a tool for membrane permeability screening. J. Pharm. Sci., 88, 28-33 (1999).
17. Augustijns, P. F., Bradshaw, T. P., and Gan, L. S., Evidence for a polarized efflux system in Caco-2 cells capable of modulating cyclosporin A transport. Biochem. Biophys. Res. Commun., 197, 360-365 (1993).
18. Li, H., Chung, S. J., and Kim, D. C, The transport of a reversible proton pump antagonist, 5,6-dimethyl-2-(4-fluoro-phenylamino)-4-(l-methyl-l,2,3,4- tetrahydroisoquinoline-2-yl) pyrimidine hydrochloride (YH1885), across Caco-2 cell monolayers. Drug Metab. Dispos., 29, 54-59 (2001).
19. Matsumoto, S.-L, Saito, H., and Inui, K.-L, Transcellular transport of oral cephalosporins in human intestinal epithelial cells, Caco-2: interaction with dipeptide transport systems in apical and basolateral membranes. J. Pharmacol. Exp. Ther., 270, 498-504 (1994).
20. Gan, L. S., Hsyu, P. H., Pritchard, J. F., and Thakker, D. R., Mechanism of intestinal absorption of ranitidine and ondansetron: transport across Caco-2 cell monolayers. Pharm. Res., 10, 1722-1725 (1993).
21. Karnes, H. T., Shiu, G., and Shah, V. P., Validation of bioanalytical methods. Pharm. Res., 8, 421-4-26 (1991).
22. Tsuji, A., and Tamai, I., Carrier-mediated intestinal transport of drugs. Pharmcol. Res., 13, 963-977 (1996).
23. Artursson, P., and Karlsson, J., Correlation between oral drug absorption in humans and apparent drug permeability coefficients in human intestinal epithelial (Caco-2) cells. Biochem. Biophys. Res. Commun., 175, 880-885 (1991).
24. Liao, K., Hoffman, R. D., and Lane, M. D., Phosphotyrosyl turnover in insulin signaling: characterization of two membrane-bound ppl5 protein tyrosine phosphatases from 3T3-L1 adipocytes. J. Biol. Chem., 266, 6544-6553 (1991).
25. Lo, Y. L., and Huang, J. D., Effects of sodium deoxycholate and sodium caprate on the transport of epirubicin in human intestinal epithelial Caco-2 cell layers and everted gut sacs of rats. Biochem. Pharmacol., 59, 665-672 (2000).
26. Richard, A., Walgren, U., Kristina, W., and Thomas, W., Transport of quercetin and its glucosides across human intestinal epithelial Caco-2 cells. Biochem. Pharmacol., 55, 1721-1727 (1998).
27. Dyer, J., Beechey, R. B., and Gorvel, J. P., Glycyl-L-Proline transport in rabbit enterocyte basolateral-membrane vesicles. Biochem. J., 269, 565-571 (1990).
28. Saito, H., and Inui, K., Dipeptide transporters in apical and basolateral membranes of the human intestinal cell line Caco-2. Am. J. Physiol., 265, 289-294 (1993).
29. Enjoh, M., Hashimoto, K., and Arai, S., Inhibitory effect of arphamenine A on intestinal dipeptide transport. Biosci. Biotechnol. Biochem., 60, 1893-1895 (1996).
30. Hashimoto, K., and Shimizu, M., Epithelial properties of human intestinal Caco-2 cells cultured in serum-free medium. Cyto-technology, 13, 175-184 (1993).
31. Satake, M., Enjoh, M., Nakamura, Y., Takano, T., Kawamura, Y., Arai, S., and Shimizu, M., Transepithelial transport of bioactive tripeptide, Val-Pro-Pro, in human intestinal Caco-2 cell monolayers. Biosci. Biotechnol. Biochem., 66, 378-384 (2002).
32. Vermeirssen, V., Deplancke, B., Tappenden, K. A., Vancamp, J., Gaskins, H. R., and Verstraete, W., Intestinal transport of the lactokinin Ala-Leu-Pro-Met-His-Ile-Arg through a Caco-2 bbe monolayer. J. Pept. Sci., 8, 95-100 (2002).
33. Quirós, A., Dóvalosb, A., Lasunción, M. A., Ramos, M., and Recio, I., Bioavailability of the antihypertensive peptide LHLPLP: transepithelial flux of HLPLP. Int. Dairy J., 18, 279-286 (2008).