메뉴 건너뛰기




Volumn 15, Issue 3, 2009, Pages 559-567

Monolayer and spheroid culture of human liver hepatocellular carcinoma cell line cells demonstrate distinct global gene expression patterns and functional phenotypes

Author keywords

[No Author keywords available]

Indexed keywords

BIOACTIVITY; CELL MEMBRANES; ELECTRON ENERGY LEVELS; GENE EXPRESSION; LIVER; MONOLAYERS; THREE DIMENSIONAL; TISSUE; TISSUE CULTURE; TISSUE ENGINEERING;

EID: 64549117445     PISSN: 19373341     EISSN: 1937335X     Source Type: Journal    
DOI: 10.1089/ten.tea.2007.0434     Document Type: Article
Times cited : (256)

References (43)
  • 1
    • 84895102676 scopus 로고    scopus 로고
    • Port, F.K., Dykstra, D.M., Merion, R.M., and Wolfe, R. 2004 Annual Report of the U.S. Organ Procurement and Transplantation Network and the Scientific Registry of Transplant Recipients: Trasplant Data 1994-2003. Department of Health and Human Services, Division of Transplantation, Rockville, MD; United Network for Organ Sharing, Richmond, VA; University Renal Research and Education Association, Ann Arbor, MI, 2004.
    • Port, F.K., Dykstra, D.M., Merion, R.M., and Wolfe, R. 2004 Annual Report of the U.S. Organ Procurement and Transplantation Network and the Scientific Registry of Transplant Recipients: Trasplant Data 1994-2003. Department of Health and Human Services, Division of Transplantation, Rockville, MD; United Network for Organ Sharing, Richmond, VA; University Renal Research and Education Association, Ann Arbor, MI, 2004.
  • 3
    • 0035137478 scopus 로고    scopus 로고
    • Human hepatocyte cell lines proliferating as cohesive spheroid colonies in alginate markedly upregulate both synthetic and detoxificatory liver function
    • Khalil, M., Shariat-Panahi, A., Tootle, R., Ryder, T., McCloskey, P., Roberts, E., Hodgson, H., and Selden, C. Human hepatocyte cell lines proliferating as cohesive spheroid colonies in alginate markedly upregulate both synthetic and detoxificatory liver function. J Hepatol 34, 68, 2001.
    • (2001) J Hepatol , vol.34 , pp. 68
    • Khalil, M.1    Shariat-Panahi, A.2    Tootle, R.3    Ryder, T.4    McCloskey, P.5    Roberts, E.6    Hodgson, H.7    Selden, C.8
  • 4
    • 20544469908 scopus 로고    scopus 로고
    • Proliferation rates of HepG2 cells encapsulated in alginate are increased in a microgravity environment compared with static cultures
    • Coward, S.M., Selden, C., Mantalaris, A., and Hodgson, H.J. Proliferation rates of HepG2 cells encapsulated in alginate are increased in a microgravity environment compared with static cultures. Artif Organs 29, 152, 2005.
    • (2005) Artif Organs , vol.29 , pp. 152
    • Coward, S.M.1    Selden, C.2    Mantalaris, A.3    Hodgson, H.J.4
  • 5
    • 0041859380 scopus 로고    scopus 로고
    • Liver tissue engineering within alginate scaffolds: Effects of cell-seeding density on hepatocyte viability, morphology, and function
    • Dvir-Ginzberg, M., Gamlieli-Bonshtein, I., Agbaria, R., and Cohen, S. Liver tissue engineering within alginate scaffolds: effects of cell-seeding density on hepatocyte viability, morphology, and function. Tissue Eng 9, 757, 2003.
    • (2003) Tissue Eng , vol.9 , pp. 757
    • Dvir-Ginzberg, M.1    Gamlieli-Bonshtein, I.2    Agbaria, R.3    Cohen, S.4
  • 6
    • 11144228413 scopus 로고    scopus 로고
    • Ultrastructural and functional investigations of adult hepatocyte spheroids during in vitro cultivation
    • Dvir-Ginzberg, M., Elkayam, T., Aflalo, E.D., Agbaria, R., and Cohen, S. Ultrastructural and functional investigations of adult hepatocyte spheroids during in vitro cultivation. Tissue Eng 10, 1806, 2004.
    • (2004) Tissue Eng , vol.10 , pp. 1806
    • Dvir-Ginzberg, M.1    Elkayam, T.2    Aflalo, E.D.3    Agbaria, R.4    Cohen, S.5
  • 7
    • 33644894164 scopus 로고    scopus 로고
    • Alginate/galactosylated chitosan/heparin scaffold as a new synthetic extracellular matrix for hepatocytes
    • Seo, S.J., Choi, Y.J., Akaike, T., Higuchi, A., and Cho, C.S. Alginate/galactosylated chitosan/heparin scaffold as a new synthetic extracellular matrix for hepatocytes. Tissue Eng 12,33, 2006.
    • (2006) Tissue Eng , vol.12 , pp. 33
    • Seo, S.J.1    Choi, Y.J.2    Akaike, T.3    Higuchi, A.4    Cho, C.S.5
  • 8
    • 38849113971 scopus 로고    scopus 로고
    • Verma, P., Verma, V., Ray, P., and Ray, A.R. Formation and characterization of three dimensional human hepatocyte cell line spheroids on chitosan matrix for in vitro tissue engineering applications. In Vitro Cell Dev Biol Anim 2007.
    • Verma, P., Verma, V., Ray, P., and Ray, A.R. Formation and characterization of three dimensional human hepatocyte cell line spheroids on chitosan matrix for in vitro tissue engineering applications. In Vitro Cell Dev Biol Anim 2007.
  • 10
    • 33846261187 scopus 로고    scopus 로고
    • Enhanced functional maturation of fetal porcine hepatocytes in threedimensional poly-L-lactic acid scaffolds: A culture condition suitable for engineered liver tissues in large-scale animal studies
    • Huang, H., Hanada, S., Kojima, N., and Sakai, Y. Enhanced functional maturation of fetal porcine hepatocytes in threedimensional poly-L-lactic acid scaffolds: a culture condition suitable for engineered liver tissues in large-scale animal studies. Cell Transplant 15, 799, 2006.
    • (2006) Cell Transplant , vol.15 , pp. 799
    • Huang, H.1    Hanada, S.2    Kojima, N.3    Sakai, Y.4
  • 13
    • 1042277975 scopus 로고    scopus 로고
    • Influence of flow conditions and matrix coatings on growth and differentiation of three-dimensionally cultured rat hepatocytes
    • Fiegel, H.C., Havers, J., Kneser, U., Smith, M.K., Moeller, T., Kluth, D., Mooney, D.J., Rogiers, X., and Kaufmann, P.M. Influence of flow conditions and matrix coatings on growth and differentiation of three-dimensionally cultured rat hepatocytes. Tissue Eng 10, 165, 2004.
    • (2004) Tissue Eng , vol.10 , pp. 165
    • Fiegel, H.C.1    Havers, J.2    Kneser, U.3    Smith, M.K.4    Moeller, T.5    Kluth, D.6    Mooney, D.J.7    Rogiers, X.8    Kaufmann, P.M.9
  • 14
    • 33750240767 scopus 로고    scopus 로고
    • Biopolymer-based biomaterials as scaffolds for tissue engineering
    • Velema, J., and Kaplan, D. Biopolymer-based biomaterials as scaffolds for tissue engineering. Adv Biochem Eng Biotechnol 102, 187, 2006.
    • (2006) Adv Biochem Eng Biotechnol , vol.102 , pp. 187
    • Velema, J.1    Kaplan, D.2
  • 15
    • 18244370254 scopus 로고    scopus 로고
    • Dexamethasone effects on rat hepatocyte spheroid formation and function
    • Abu-Absi, S.F., Hu, W.S., and Hansen, L.K. Dexamethasone effects on rat hepatocyte spheroid formation and function. Tissue Eng 11, 415, 2005.
    • (2005) Tissue Eng , vol.11 , pp. 415
    • Abu-Absi, S.F.1    Hu, W.S.2    Hansen, L.K.3
  • 16
    • 0025135443 scopus 로고
    • Formation of multicellular spheroids composed of adult rat hepatocytes in dishes with positively charged surfaces and under other nonadherent environments
    • Koide, N., Sakaguchi, K., Koide, Y., Asano, K., Kawaguchi, M., Matsushima, H., Takenami, T., Shinji, T., Mori, M., and Tsuji, T. Formation of multicellular spheroids composed of adult rat hepatocytes in dishes with positively charged surfaces and under other nonadherent environments. Exp Cell Res 186, 227, 1990.
    • (1990) Exp Cell Res , vol.186 , pp. 227
    • Koide, N.1    Sakaguchi, K.2    Koide, Y.3    Asano, K.4    Kawaguchi, M.5    Matsushima, H.6    Takenami, T.7    Shinji, T.8    Mori, M.9    Tsuji, T.10
  • 17
    • 34547124838 scopus 로고    scopus 로고
    • Identification and characterization of a novel prespheroid 3-dimensional hepatocyte monolayer on galactosylated substratum
    • Du, Y., Han, R., Ng, S., Ni, J., Sun, W., Wohland, T., Ong, S.H., Kuleshova, L., and Yu, H. Identification and characterization of a novel prespheroid 3-dimensional hepatocyte monolayer on galactosylated substratum. Tissue Eng 13, 1455, 2007.
    • (2007) Tissue Eng , vol.13 , pp. 1455
    • Du, Y.1    Han, R.2    Ng, S.3    Ni, J.4    Sun, W.5    Wohland, T.6    Ong, S.H.7    Kuleshova, L.8    Yu, H.9
  • 20
    • 0031904597 scopus 로고    scopus 로고
    • Growing tissues in microgravity
    • Unsworth, B.R., and Lelkes, P.I. Growing tissues in microgravity. Nat Med 4, 901, 1998.
    • (1998) Nat Med , vol.4 , pp. 901
    • Unsworth, B.R.1    Lelkes, P.I.2
  • 21
    • 0034807979 scopus 로고    scopus 로고
    • Optimized suspension culture: The rotating-wall vessel
    • Hammond, T.G., and Hammond, J.M. Optimized suspension culture: the rotating-wall vessel. Am J Physiol Renal Physiol 281, F12, 2001.
    • (2001) Am J Physiol Renal Physiol , vol.281
    • Hammond, T.G.1    Hammond, J.M.2
  • 22
    • 33745766692 scopus 로고    scopus 로고
    • Enhancing the drug metabolism activities of C3A-a human hepatocyte cell line-by tissue engineering within alginate scaffolds
    • Elkayam, T., Amitay-Shaprut, S., Dvir-Ginzberg, M., Harel, T., and Cohen, S. Enhancing the drug metabolism activities of C3A-a human hepatocyte cell line-by tissue engineering within alginate scaffolds. Tissue Eng 12, 1357, 2006.
    • (2006) Tissue Eng , vol.12 , pp. 1357
    • Elkayam, T.1    Amitay-Shaprut, S.2    Dvir-Ginzberg, M.3    Harel, T.4    Cohen, S.5
  • 23
    • 13444276396 scopus 로고    scopus 로고
    • Signal transduction and mechanical stress
    • Hughes-Fulford, M. Signal transduction and mechanical stress. Sci STKE 2004, RE12, 2004.
    • (2004) Sci STKE , vol.2004
    • Hughes-Fulford, M.1
  • 24
    • 33744488545 scopus 로고    scopus 로고
    • Cellular mechanotransduction: Putting all the pieces together again
    • Ingber, D.E. Cellular mechanotransduction: putting all the pieces together again. Faseb J 20, 811, 2006.
    • (2006) Faseb J , vol.20 , pp. 811
    • Ingber, D.E.1
  • 25
    • 33646867022 scopus 로고    scopus 로고
    • Fibroblast growth factor-2 is an immediate-early gene induced by mechanical stress in osteogenic cells
    • Li, C.F., and Hughes-Fulford, M. Fibroblast growth factor-2 is an immediate-early gene induced by mechanical stress in osteogenic cells. J Bone Miner Res 21, 946, 2006.
    • (2006) J Bone Miner Res , vol.21 , pp. 946
    • Li, C.F.1    Hughes-Fulford, M.2
  • 26
    • 0027275495 scopus 로고
    • A microassay for measuring cytochrome P450IA1 and P450IIB1 activities in intact human and rat hepatocytes cultured on 96-well plates
    • Donato, M.T., Gomez-Lechon, M.J., and Castell, J.V. A microassay for measuring cytochrome P450IA1 and P450IIB1 activities in intact human and rat hepatocytes cultured on 96-well plates. Anal Biochem 213, 29, 1993.
    • (1993) Anal Biochem , vol.213 , pp. 29
    • Donato, M.T.1    Gomez-Lechon, M.J.2    Castell, J.V.3
  • 28
    • 0028870496 scopus 로고
    • Hormonal regulation of albumin gene expression in primary cultures of rat hepatocytes
    • Kimball, S.R., Horetsky, R.L., and Jefferson, L.S. Hormonal regulation of albumin gene expression in primary cultures of rat hepatocytes. Am J Physiol 268, E6, 1995.
    • (1995) Am J Physiol , vol.268
    • Kimball, S.R.1    Horetsky, R.L.2    Jefferson, L.S.3
  • 29
    • 0023128221 scopus 로고
    • Stimulation of albumin gene transcription by insulin in primary cultures of rat hepatocytes
    • Lloyd, C.E., Kalinyak, J.E., Hutson, S.M., and Jefferson, L.S. Stimulation of albumin gene transcription by insulin in primary cultures of rat hepatocytes. Am J Physiol 252, C205, 1987.
    • (1987) Am J Physiol , vol.252
    • Lloyd, C.E.1    Kalinyak, J.E.2    Hutson, S.M.3    Jefferson, L.S.4
  • 30
    • 0021089020 scopus 로고
    • Selective block of albumin gene expression in chick embryo hepatocytes cultured without hormones and its partial reversal by insulin
    • Plant, P.W., Deeley, R.G., and Grieninger, G. Selective block of albumin gene expression in chick embryo hepatocytes cultured without hormones and its partial reversal by insulin. J Biol Chem 258, 15355, 1983.
    • (1983) J Biol Chem , vol.258 , pp. 15355
    • Plant, P.W.1    Deeley, R.G.2    Grieninger, G.3
  • 31
    • 0022065448 scopus 로고
    • Posttranscriptional regulation of albumin gene expression in Xenopus liver
    • Kazmaier, M., Bruning, E., and Ryffel, G.U. Posttranscriptional regulation of albumin gene expression in Xenopus liver. Embo J 4, 1261, 1985.
    • (1985) Embo J , vol.4 , pp. 1261
    • Kazmaier, M.1    Bruning, E.2    Ryffel, G.U.3
  • 32
    • 0034538443 scopus 로고    scopus 로고
    • Three dimensional culture upregulates extracellular matrix protein expression in human liver cell lines-a step towards mimicking the liver in vivo?
    • Selden, C., Khalil, M., and Hodgson, H. Three dimensional culture upregulates extracellular matrix protein expression in human liver cell lines-a step towards mimicking the liver in vivo? Int J Artif Organs 23, 774, 2000.
    • (2000) Int J Artif Organs , vol.23 , pp. 774
    • Selden, C.1    Khalil, M.2    Hodgson, H.3
  • 33
    • 1842426730 scopus 로고    scopus 로고
    • Cell shape, cytoskeletal tension, and RhoA regulate stem cell lineage commitment
    • McBeath, R., Pirone, D.M., Nelson, C.M., Bhadriraju, K., and Chen, C.S. Cell shape, cytoskeletal tension, and RhoA regulate stem cell lineage commitment. Dev Cell 6, 483, 2004.
    • (2004) Dev Cell , vol.6 , pp. 483
    • McBeath, R.1    Pirone, D.M.2    Nelson, C.M.3    Bhadriraju, K.4    Chen, C.S.5
  • 34
    • 0028557342 scopus 로고
    • Extracellular matrix-dependent tissue-specific gene expression in mammary epithelial cells requires both physical and biochemical signal transduction
    • Roskelley, C.D., Desprez, P.Y., and Bissell, M.J. Extracellular matrix-dependent tissue-specific gene expression in mammary epithelial cells requires both physical and biochemical signal transduction. Proc Natl Acad Sci U S A 91, 12378, 1994.
    • (1994) Proc Natl Acad Sci U S A , vol.91 , pp. 12378
    • Roskelley, C.D.1    Desprez, P.Y.2    Bissell, M.J.3
  • 35
    • 2642666080 scopus 로고
    • Cell shape controls terminal differentiation of human epidermal keratinocytes
    • Watt, F.M., Jordan, P.W., and O'Neill, C.H. Cell shape controls terminal differentiation of human epidermal keratinocytes. Proc Natl Acad Sci U S A 85, 5576, 1988.
    • (1988) Proc Natl Acad Sci U S A , vol.85 , pp. 5576
    • Watt, F.M.1    Jordan, P.W.2    O'Neill, C.H.3
  • 36
    • 0032881990 scopus 로고    scopus 로고
    • Geometric control of switching between growth, apoptosis, and differentiation during angiogenesis using micropatterned substrates
    • Dike, L.E., Chen, C.S., Mrksich, M., Tien, J., Whitesides, G.M., and Ingber, D.E. Geometric control of switching between growth, apoptosis, and differentiation during angiogenesis using micropatterned substrates. In Vitro Cell Dev Biol Anim 35, 441, 1999.
    • (1999) In Vitro Cell Dev Biol Anim , vol.35 , pp. 441
    • Dike, L.E.1    Chen, C.S.2    Mrksich, M.3    Tien, J.4    Whitesides, G.M.5    Ingber, D.E.6
  • 38
    • 34548321321 scopus 로고    scopus 로고
    • Cadherins, RhoA, and Rac1 are differentially required for stretch-mediated proliferation in endothelial versus smooth muscle cells
    • Liu, W.F., Nelson, C.M., Tan, J.L., and Chen, C.S. Cadherins, RhoA, and Rac1 are differentially required for stretch-mediated proliferation in endothelial versus smooth muscle cells. Circ Res 101, e44, 2007.
    • (2007) Circ Res , vol.101
    • Liu, W.F.1    Nelson, C.M.2    Tan, J.L.3    Chen, C.S.4
  • 39
    • 34648837293 scopus 로고    scopus 로고
    • Culture of HepG2 liver cells on three dimensional polystyrene scaffolds enhances cell structure and function during toxicological challenge
    • Bokhari, M., Carnachan, R.J., Cameron, N.R., and Przyborski, S.A. Culture of HepG2 liver cells on three dimensional polystyrene scaffolds enhances cell structure and function during toxicological challenge. J Anat 211, 567, 2007.
    • (2007) J Anat , vol.211 , pp. 567
    • Bokhari, M.1    Carnachan, R.J.2    Cameron, N.R.3    Przyborski, S.A.4
  • 40
    • 33749060495 scopus 로고    scopus 로고
    • Identification and functional analysis of candidate genes regulating mesenchymal stem cell self-renewal and multipotency
    • Song, L., Webb, N.E., Song, Y., and Tuan, R.S. Identification and functional analysis of candidate genes regulating mesenchymal stem cell self-renewal and multipotency. Stem Cells 24, 1707, 2006.
    • (2006) Stem Cells , vol.24 , pp. 1707
    • Song, L.1    Webb, N.E.2    Song, Y.3    Tuan, R.S.4
  • 41
    • 27744455423 scopus 로고    scopus 로고
    • Identification of genes specific to ''oval cells'' in the rat 2-acetylaminofluorene/partial hepatectomy model
    • Batusic, D.S., Cimica, V., Chen, Y., Tron, K., Hollemann, T., Pieler, T., and Ramadori, G. Identification of genes specific to ''oval cells'' in the rat 2-acetylaminofluorene/partial hepatectomy model. Histochem Cell Biol 124, 245, 2005.
    • (2005) Histochem Cell Biol , vol.124 , pp. 245
    • Batusic, D.S.1    Cimica, V.2    Chen, Y.3    Tron, K.4    Hollemann, T.5    Pieler, T.6    Ramadori, G.7
  • 42
    • 33747337426 scopus 로고    scopus 로고
    • Characterization of two distinct liver progenitor cell subpopulations of hematopoietic and hepatic origins
    • Corcelle, V., Stieger, B., Gjinovci, A., Wollheim, C.B., and Gauthier, B.R. Characterization of two distinct liver progenitor cell subpopulations of hematopoietic and hepatic origins. Exp Cell Res 312, 2826, 2006.
    • (2006) Exp Cell Res , vol.312 , pp. 2826
    • Corcelle, V.1    Stieger, B.2    Gjinovci, A.3    Wollheim, C.B.4    Gauthier, B.R.5
  • 43
    • 33745814436 scopus 로고    scopus 로고
    • Signalling and functional diversity within the Axl subfamily of receptor tyrosine kinases
    • Hafizi, S., and Dahlback, B. Signalling and functional diversity within the Axl subfamily of receptor tyrosine kinases. Cytokine Growth Factor Rev 17, 295, 2006.
    • (2006) Cytokine Growth Factor Rev , vol.17 , pp. 295
    • Hafizi, S.1    Dahlback, B.2


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.