Microbial testing in periodontics: Value, limitations and future directions

Periodontology 2000

Volumn 50, Issue 1, 2009, Pages 25-38

  Shaddox, Luciana M ^a   Walker, Clay ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

ANTIINFECTIVE AGENT;

ARTICLE; BACTERIUM; CLASSIFICATION; DRUG EFFECT; HUMAN; METHODOLOGY; MICROBIOLOGICAL EXAMINATION; MICROBIOLOGY; MULTIMODALITY CANCER THERAPY; PATHOGENICITY; PERIODONTAL DISEASE; PERIODONTICS;

ANTI-BACTERIAL AGENTS; BACTERIA; COMBINED MODALITY THERAPY; HUMANS; MICROBIOLOGICAL TECHNIQUES; PERIODONTAL DISEASES; PERIODONTICS;

EID: 64549112244     PISSN: 09066713     EISSN: 16000757     Source Type: Journal    
DOI: 10.1111/j.1600-0757.2008.00285.x     Document Type: Article

References (83)

1. Al-Mubarak S, Al-Nowaiser A, Rass MA, Alsuwyed A, Alghofili A, Al-Mubarak EK. Antibiotic prescription and dental practice in Saudi Arabia: the need to reinforce guidelines and implement specialty needs. J Int Acad Periodontol 2004 : 6 : 47 55.
2. Albandar JM, Lyngatadaas SP, Forbord B. PCR primers for the amplification of the 16S rRNA gene of oral bacteria and for the specific identification of Actinobacillus actinomycetemcomitans. Eur J Oral Sci 1996 : 104 : 144 147.
3. Badersten A, Nilveus R, Elgelberg J. Effect of nonsurgical periodontal therapy. I. Moderately advanced periodontitis. J Clin Periodontol 1981 : 8 : 57 72.
4. Badersten A, Nilveus R, Egelberg J. Effect of nonsurgical periodontal therapy. II. Severely advanced periodontitis. J Clin Periodontol 1984 : 11 : 63 76.
5. Barretto-Tinoco EM, Stevens R, Haubek D, Lai CH, Balachandran S, Preus H. Relationship of serotype, leukotoxin gene type, and lysogeny in Actinobacillus actinomycetemcomitans. Eur J Oral Sci 1997 : 105 : 310 317.
6. Brito LCN, Teles FR, Teles RP, Franca EC, Ribeiro-Sobrinh AP, Haffajee AD, Socransky SS. Use of mutiple-displacement amplification and checkerboard DNA-DNA hybridization to examine the microbiota of endodontic infections. J Clin Microbiol 2007 : 45 : 3039 3049.
7. Caffesse RG, Sweeney PL, Smith BA. Scaling and root planing with and without periodontal flap surgery. J Clin Periodontol 1986 : 13 : 205 210.
8. Call DR, Bakko MK, Krug MJ, Roberts MC. Identifying antimicrobial resistance genes with DNA microarrays. Antimicrob Agents Chemother 2003 : 47 : 3290 3295.
9. Chaves ES, Jeffcoat MK, Ryerson CC, Snyder B. Persistent bacterial colonization of Porphyromonas gingivalis, Prevotella intermedia, and Actinobacillus actinomycetemcomitans in periodontitis and its association with alveolar bone loss after 6 months of therapy. J Clin Periodontol 2000 : 27 : 897 903.
10. Choudhury M, Needleman I, Gillam D, Moles DR. Systemic and local antimicrobial use in periodontal therapy in England and Wales. J Clin Periodontol 2001 : 28 : 833 839.
11. Christersson LA, Wikesjö UME, Albini B, Zambon JJ, Genco RJ. Tissue localization of Actinobacillus actinomycetemcomitans in human periodontitis. J Periodontol 1987 : 58 : 540 545.
12. Cobb CM. Non-surgical pocket therapy: mechanical. Ann Periodontol 1996 : 1 : 443 490.
13. Cohen L, Rams T, Slots J, Walker C. Independent analyses of microbiological samples by three testing laboratories. J Dent Res 2001 : 80 : 219. Abstr. 1465.
14. Colombo AP, Haffajee AD, DeWhirst FE, Paster BJ, Smith CM, Cugini MA, Socransky SS. Clinical and microbiological features of refractory periodontitis subjects. J Clin Periodontol 1998 : 25 : 169 180.
15. Demirbas F, Gjermo PE, Preus HR. Antibiotic prescribing practices among Norwegian dentists. Acta Odontol Scand 2006 : 64 : 355 359.
16. Fine DH. Microbial identification and antibiotic sensitivity testing, an aid for patients refractory to periodontal therapy. J Clin Periodontol 1994 : 21 : 98 106.
17. Fine DH, Markowitz V, Furgang D, Fairlie K, Ferrandiz J, Nasri C. Aggregatibacter actinomycetemcomitans and its relationship to initiation of localized aggressive periodontitis: longitudinal cohort study of initially healthy adolescents. J Clin Microbiol 2007 : 45 : 3859 3869.
18. Gajardo M, Silva N, Gomez L, Leon R, Parra B, Contreras A. Prevalace of periodontopathic bacteria in aggressive periodontitis patients in a Chilean population. J Periodontol 2005 : 76 : 289 294.
19. Gomi K, Yashima A, Iino F, Kanazaashi M, Nagano T, Shibukawa N. Drug concentration in inflamed periodontal tissues after systemic administrated azithromycin. J Periodontol 2007 : 78 : 918 923.
20. Haffajee AD, Socransky SS, Gunsolley JC. Systemic anti-infective periodontal therapy. A systematic review. Ann Periodontol 2003 : 8 : 115 181.
21. Haffajee AD, Dibart S, Kent RL, Socransky SS. Factors associated with different responses to periodontal therapy. J Clin Periodontol 1995 : 22 : 628 636.
22. Haffajee AD, Cugini MA, Dibart S, Smith C, Kent RL, Socransky SS. Clinical and microbiological features of subjects with adult periodontitis who responded poorly to scaling and root planing. J Clin Periodontol 1997 : 24 : 767 776.
23. Haubek D, Poulsen K, Westergaard J, Dahlén G, Kilian M. Highly toxic clone of Actinobacillus actinomycetemcomitans in geographically widespread cases of juvenile periodontitis in adolescents of African origin. J Clin Microbiol 1996 : 34 : 1576 1578.
24. Haubek D, Dirienzo JM, Tinoco EM, Westergaard J, Lopez NJ, Chung CP. Racial tropism of a highly toxic clone of Actinobacillus actinomycetemcomitans associated with juvenile periodontitis. J Clin Microbiol 1997 : 35 : 3037 3042.
25. Herrera D, Sanz M, Jepsen S, Needleman I, Roldán S. A systematic review on the effect of systemic antibiotics as an adjunct to scaling and root planing in periodontitis patients. J Clin Periodontol 2002 : 29 : 136 159.
26. Jacoby GA. ß-lactamase nomenclature. Antimicrob Agents Chemother 2006 : 50 : 1123 1129.
27. Kaldahl WB, Kalkwarf KL, Patil KD, Dyer JK, Bates RE. Evaluation of four modalities of periodontal therapy. Mean probing depth, probing attachment level and recession changes. J Periodontol 1988 : 59 : 783 793.
28. Kumar PS, Griffen AL, Moeschberger ML, Leys EJ. Identification of candidate periodontal pathogens and beneficial species by quantitative 16S clonal analysis. J Clin Microbiol 2005 : 43 : 3944 3955.
29. Kumar MS, Griffen AL, Barton JA, Paster BJ, Moeschberger ML, Leys EJ. New bacterial species associated with chronic periodontitis. J Dent Res 2003 : 82 : 338 344.
30. Kuriyama T, Williams DW, Yanagisawa M, Iwahara K, Shimizu C, Nakagawa K. Antimicrobial susceptibility of 800 anaerobic isolates from patients with dentoalveolar infection to 13 oral antibiotics. Oral Microbiol Immunol 2007 : 22 : 285 288.
31. Lamell CW, Griffen AL, McCellan DL, Leys EJ. Acquisition and colonization stability of Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis in children. J Clin Microbiol 2000 : 38 : 1196 1199.
32. Levi D, Csima A, Birek P, Ellen RP, McCulloch C. Impact of microbiological diagnostic tools in the management of periodontal disease. Res Microbiol 1993 : 152 : 631 639.
33. Listgarten MA, Loomer PM. Microbial identification in the management of periodontal disease. A systematic review. Ann Periodontol 2003 : 8 : 182 192.
34. Löe H, Theilade E, Jensen SB. Experimental gingivitis in man. J Periodontol 1965 : 36 : 177 187.
35. Loomer PM. Microbial diagnostic testing in the treatment of periodontal diseases. Periodontol 2000 2004 : 34 : 49 56.
36. Maeda H, Fujimoto C, Haruki Y, Maeda T, Kokeguchi S, Petelin M, Arai H, Tanimoto I, Nishimura F, Takashiba S. Quantitative real-time PCR using TaqMan and SYBR Green for Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, tetQ gene and total bacteria. FEMS Immunol Med Microbiol 2003 : 39 : 81 86.
37. Mellado JR, Freedman AL, Salkin LM, Stein MD, Schneider DB, Cutler RH. The clinical relevance of microbiologic testing: a comparative analysis of microbiologic samples secured from the same sites and cultured in two independent laboratories. Int J Periodontics Restorative Dent 2001 : 21 : 232 239.
38. Mikx FH, Konig KG, van Rossum GM, Bruers JJ. Prescription of antibiotics for the prevention of bacterial endocarditis by dentists in the Netherlands. Nederlands Tijdschrift Voor Tandheelkunde 1995 : 102 : 337 339.
39. Mombelli A, van Winkelhoff AJ. The systemic use of antibiotics in periodontal therapy: Second European Workshop in Periodontology. Berne, Switzerland : Quintessence, 1996.
40. Mombelli A, Gmur R, Gobbi C, Lang NP. Actinobacillus actenomycetemcomitans in adult periodontitis. II. Characterization of isolated strains and effect of mechanical periodontal treatment. J Periodontol 1994 : 65 : 827 834.
41. Mombelli A, Gmur R, Gobbi C, Lang NP. Actinobacillus actinomycetemcomitans in adult periodontitis.I. Topographic distribution before and after treatment. J Periodontol 1994 : 65 : 820 826.
42. Mombelli A, Schmid B, Rutar A, Lang NP. Persistent patterns of Porphyromonas gingivalis, Prevotella intermedia/nigrescens, and Actinobacillus actinomycetemcomitans after mechanical therapy of periodontal disease. J Periodontol 2000 : 71 : 14 21.
43. Olsvik B, Hansen BF, Tenover FC, Olsen I. Tetracycline-resistant microorganisms recovered from patients with refractory periodontal disease. J Clin Periodontol 1995 : 22 : 391 396.
44. Pajukanta R, Asikainen S, Saarela M, Alauusua S, Jousimies-Somer H. In vitro antimicrobial susceptibility of different serotypes of Actinobacillus actinomycetemcomitans. Scand J Dent Res 1993 : 101 : 299 303.
45. Pallasch TJ. Pharmacokinetic principles of antimicrobial therapy. Periodontol 2000 1996 : 10 : 5 11.
46. Paster BJ, Boches SK, Galvin JL, Ericson RE, Lau CN, Levanos VA, Sahasrabudhe A, Dewhirst FE. Bacterial diversity in human subgingival plaque. J Bacteriol 2001 : 183 : 3370 3783.
47. Rams TE, Babalola OO, Slots J. Subgingival occurrence of enteric rods, yeasts, and staphylococci after systemic doxycycline therapy. Oral Microbiol Immunol 1990 : 5 : 166 168.
48. Rams TE, Listgarten MA, Slots J. Utility of 5 major putative periodontal pathogens and selected clinical parameters to predict periodontal breakdown in patients on maintenance care. J Clin Periodontol 1996 : 23 : 346 354.
49. Roberts MC. Update on acquired tetracycline resistance genes. FEMS Microbiol Lett 2005 : 245 : 195 230.
50. Rosenberg ES, Torosian JP, Hammond BF, Cutler SA. Routine anaerobic bacterial culture and systemic antibiotic usage in the treatment of adult periodontitis: a 6-year longitudinal study. Int J Periodontics Restorative Dent 1993 : 13 : 213 243.
51. Sakamoto M, Umeda M, Ishikawa I, Benno Y. Comparison of the oral bacterial flora in saliva from a healthy subjects and two periodontitis patients by sequence analysis of 16S rDNA libraries. Microbiol Immunol 2000 : 44 : 643 652.
52. Sakamoto M, Huang Y, Ohnishi M, Umeda M, Ishikawa I, Benno Y. Changes in oral microbial profiles after periodontal treatment as determined by molecular analysis of 16S rRNA genes. J Med Microbiol 2004 : 53 : 563 571.
53. Sakellari D, Goodson JM, Kolokotronis A, Kostantinidis A. Concentration of 3 tetracyclines in plasma, gingival crevice fluid, and saliva. J Clin Periodontol 2000 : 27 : 53 60.
54. Salkin LM, Freedman AL, Mellado JR, Stein MD, Schneider DB, Butler L. The clinical relevance of microbiologic testing. Part 2: a comparative analysis of microbiologic samples secured simultaneously from the same sites and cultured in the same laboratory. Int J Periodontics Restorative Dent 2003 : 23 : 121 127.
55. Sato K, Yoneyama T, Okamoto H, Dahlén G, Lindhe J. The effect of subgingival debridement on periodontal disease parameters and the subgingival microbiota. J Clin Periodontol 1993 : 20 : 359 365.
56. Sedlacek M, Walker C. Antibiotic resistance in an in vitro subgingival biofilm model. Oral Microbiol Immunol 2007 : 22 : 333 339.
57. Simonson LG, Robinson PJ, Pranger RJ, Cohen ME, Morton HE. Treponema denticola and Porphyromonas gingivalis as prognostic markers following periodontal treatment. J Periodontol 1992 : 63 : 270 273.
58. Slots J. Systemic antibiotics in periodontitics. J Periodontol 2004 : 75 : 1553 1565.
59. Slots J, Rams TE. Antibiotics in periodontal therapy: advantages and disadvantages. J Clin Periodontal 1990 : 17 (pt2 479 493.
60. Slots J, Ting M. Systemic antibiotic in the treatment of periodontal diseases. Periodontol 2000 2002 : 28 : 106 176.
61. Slots J, Ashimoto A, Flynn MJ, Li G, Chen C. Detection of putative periodontal pathogens in subgingival specimens by 16S ribosomal DNA amplification with the polymerase chain reaction. Clin Infect Dis 1995 : 20 : S304 S307.
62. Socransky SS, Haffajee AD. Periodontal microbial ecology. Periodontol 2000 2005 : 38 : 135 187.
63. Socransky SS, Haffajee AD, Cugini MA, Smith C, Kent RL. Microbial complexes in subgingival plaque. J Clin Periodontol 1998 : 25 : 134 144.
64. Socransky SS, Smith C, Martin L, Paster BJ, Dewhirst FE, Levin AE. "Checkerboard" DNA-DNA hybridization. Biotechniques 1994 : 17 : 488 492.
65. Socransky S, Haffajee A, Smith C, Martin L, Haffajee J, Uzel N, Goodson J. Use of checkerboard DNA-DNA hybridization to study complex microbial ecosystems. Oral Microbiol Immunol 2004 : 19 : 352 362.
66. Suchett-Kaye G, Morrier JJ, Barsotti O. Clinical usefulness of microbiological dianostic tools in the management of periodontal disease. Res Microbiol 2001 : 152 : 631 639.
67. Sweeney EA, Alcoforado GA, Nyman S, Slots J. Prevalence and microbiology of localized prepubertal periodontitis. Oral Microbiol Immunol 1987 : 2 : 65 70.
68. Teles FR, Haffajee AD, Socransky SS. Multiple-displacement as an aid in checkerboard DNA-DNA hybridization. Oral Microbiol Immunol 2007 : 22 : 118 125.
69. Theilade E. The experimental gingivitis studies: the microbiological perspective. J Dent Res 1996 : 75 : 1434 1438.
70. Theilade E, Wright WH, Jensen SB, Löe H. Experimental gingivitis in man. A longitudinal clinical and bacteriological investigation. J Periodontal Res 1966 : 1 : 1 13.
71. Thiha K, Takeuchi Y, Umeda M, Huang Y, Ohnishi M, Ishikawa I. Identification of periodontopathic bacteria in gingival tissue of Japanese periodontitis patients. Oral Microbiol Immunol 2007 : 22 : 201 207.
72. Tran SD, Rudney JD. Multplex PCR using conserved species-specific 16S rRNA gene primers for simultaneous detection of Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis. J Clin Microbiol 1996 : 34 : 2674 2678.
73. Tran SD, Rudney JD. Improved multiplex PCR using conserved and species-specific 16S rRNA gene primers for simultaneous detection of Actinobacillus actinomycetemicomitans, Bacteroides forsythus, and Porphyromonas gingivalis. J Clin Microbiol 1999 : 37 : 3504 3508.
74. Wahlfors J, Meurman JH, Väisänen P, Alakuijala P, Korhonen A, Torkko H, Jänne J. Simultaneous detection of Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis by a rapid PCR method. J Dent Res 1995 : 74 : 1796 1801.
75. Walker CB. The acquisition of antibiotic resistance in the periodontal microflora. Periodontol 2000 1996 : 10 : 79 88.
76. Walker SL, Ash MM. A study of root planing by scanning electron microscopy. Dent Hyg 1976 : 50 : 109 114.
77. Walker C, Karpinia K. Rational for the use of antibiotics in periodontics. J Periodontol 2002 : 73 : 1189 1197.
78. Walker C, Machion L,. Antibiotics. In : Ciancio S, editor. ADA/PDR Guide to Dental Therapeutics, 4th edn. Chicago : American Dental Assoc, 2007 : 161 216.
79. Walker CB, Gordon JM, Socransky SS. Antibiotic susceptibility testing of subgingival plaque samples. J Clin Periodontol 1983 : 10 : 422 432.
80. Walker C, Karpinia K, Baehni P. Chemotherapeutics: antibiotics and other antimicrobials. Periodontol 2000 2004 : 36 : 146 165.
81. Yang HW, Asikainen S, Dogan B, Suda R, Lai CH. Relationship of Actinobacillus actinomycetemcomitans serotype b to aggressive periodontitis: frequency in pure culture infection. J Periodontol 2004 : 75 : 592 599.
82. Zambon JJ. Principles of the evaluation of the diagnostic value of subgingival bacteria. Ann Periodontol 1997 : 2 : 138 148.
83. Zambon JJ, Christerrsson LA, Slots J. Actinobacillus actinomycetemcomitans in human periodontal disease. Prevalence in patient groups and distribution of biotypes and serotypes within families. J Periodontol 1983 : 54 : 707 711.