Multiplex PCR using conserved and species-specific 16S rRNA gene primers for simultaneous detection of Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis

Journal of Clinical Microbiology

Volumn 34, Issue 11, 1996, Pages 2674-2678

  Tran, Simon Dangtuan ^a   Rudney, Joel D ^a  

^a UNIVERSITY OF MINNESOTA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

RNA 16S;

ACTINOBACILLUS ACTINOMYCETEMCOMITANS; ARTICLE; BACTERIAL GENE; BACTERIUM DETECTION; MOUTH FLORA; PERIODONTITIS; POLYMERASE CHAIN REACTION; PORPHYROMONAS; PRIORITY JOURNAL;

ACTINOBACILLUS ACTINOMYCETEMCOMITANS; BACTERIA (MICROORGANISMS); PORPHYROMONAS GINGIVALIS;

EID: 0029964777     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/jcm.34.11.2674-2678.1996     Document Type: Article

References (36)

1. Albandar, J. M., and S. P. Lyngstadaas. 1995. PCR primers for the detection of Actinobacillus actinomycetemcomitans, abstr. 1299. J. Dent. Res. 74:174.
2. Alpagot, T., L. F. Wolff, Q. T. Smith, S. D. Tran, D. M. Aeppli, and N. A. Hardie. Risk indicators for periodontal disease in a racially diverse urban population. J. Clin. Periodontol., in press.
3. Bodinka, A., H. Schmidt, B. Henkel, T. F. Flemmig, B. Klaiber, and H. Karen. 1994. Polymerase chain reaction for the identification of Porphyromonas gingivalis collagenase genes. Oral Microbiol. Immunol. 9:161-165.
4. Chamberlain, J. S., and J. R. Chamberlain. 1994. Optimization of multiplex PCRs, p. 38-46. In K. B. Mullis, F. Ferre, and R. A. Gibbs (ed.), The polymerase chain reaction. Birkhauser, Boston.
5. Chamberlain, J. S., R. A. Gibbs, J. E. Ranier, and C. T. Caskey. 1990. Multiplex PCR for the diagnosis of Duchenne muscular dystrophy, p. 272-281. In M. Innis, D. Gelfand, J. Sninski, and T. While (ed.), PCR protocols: a guide to methods and applications. Academic Press, Orlando, Fla.
6. Choi, B. K., B. J. Paster, F. E. Dewhirst, and U. B. Göbel. 1994. Diversity of cultivable and uncultivable oral spirochetes from a patient with severe destructive periodontitis. Infect. Immun. 62:1889-1895.
7. Chuba, P. J., K. Pelz, G. Krekeler, T. S. De Isele, and U. Göbel. 1988. Synthetic oligodeoxynucleotide probes for the rapid detection of bacteria associated with human periodontitis. J. Gen. Microbiol. 134:1931-1938.
8. Dale, P., J. Johnson, and C. Schachtele. 1996. Detection of fimbrial genes in Actinomyces with polymerase chain reaction, abstr. 609. J. Dent. Res. 75:94.
9. Dewhirst, F. E., and B. J. Paster. 1991. DNA probe analyses for the detection of periodontopathic bacteria in clinical samples, p. 367-377. In S. Hamada, S. C. Holt, and J. R. McGhee (ed.), Periodontal disease: pathogens and host immune responses. Quintessence, Tokyo.
10. Dix, K., S. M. Watanabe, S. McArdle, D. I. Lee, C. Randolph, B. Moncia, and D. E. Schwartz. 1990. Species-specific oligodeoxynucleotide probes for the identification of periodontal bacteria. J. Clin. Microbiol. 28:319-323.
11. Evans, M. J., R. T. Evans, and S. J. Greenberg. 1993. Detection of Porphyromonas (Bacteroides) gingivalis using PCR, p. 609-619. In G. D. Ehrlich and S. J. Greenberg (ed.), PCR-based diagnostics in infectious disease. Blackwell Scientific Publications, Boston.
12. Flemmig, T. F., S. Rüdiger, U. Hofmann, H. Schmidt, B. Plaschke, A. Strätz, B. Klaiber, and H. Karch. 1995. Identification of Actinobacillus actinomycetemcomitans in subgingival plaque by PCR. J. Clin. Microbiol. 33:3102-3105.
13. Gelfand, D. H., and T. J. While. 1990. Thermostable DNA polymerases, p. 129-141. In M. Innis, D. Gelfand, J. Sninski, and T. While (ed.), PCR protocols: a guide to methods and applications. Academic Press. Orlando, Fla.
14. Goncharoff, P., D. H. Figurski, R. H. Stevens, and D. H. Fine. 1993. Identification of Actinobacillus actinomycetemcomitans: polymerase chain reaction amplification of IktA-specific sequences. Oral Microbiol. Immunol. 8:105-110.
15. Haffajee, A. D., and S. S. Socransky. 1994. Microbial etiological agents of destructive periodontal diseases. Periodontology 2000 5:78-111.
16. Kwok, S., and R. Higuchi. 1984. Avoiding false positives with PCR. Nature (London) 339:237-238.
17. Larsen, N., G. J. Olsen, B. L. Maidak, M. J. McCaughey, R. Overbeek, T. J. Macke, T. L. Marsh, and C. R. Woese. 1993. The ribosomal database project. Nucleic Acids Res. 21(Suppl.):3021-3023.
18. Leser, T. D. 1995. Quantitation of Pseudomonas sp. strain B13(FR1) in the marine environment by competitive polymerase chain reaction. J. Microbiol. Methods 22:249-262.
19. Leys, E. J., A. L. Griffen, S. J. Strong, and P. A. Fuerst. 1994. Detection and strain identification of Actinobacillus actinomycetemcomitans by nested PCR. J. Clin. Microbiol. 32:1288-1294.
20. Loesche, W. J., D. E. Lopatin, J. Stoll, N. van Poperin, and P. P. Hujoel. 1992. Comparison of various detection methods for periodontopathic bacteria: can culture be considered the primary reference standard? J. Clin. Microbiol. 30:418-426.
21. Longo, M. C., M. S. Berninger, and J. L. Hartley. 1990. Use of uracil DNA glycosylase to control carry-over contamination in polymerase chain reaction. Gene 93:125-128.
22. Loos, B. G., D. W. Dyer, R. G. Genco, R. K. Selander, and D. P. Dickinson. 1993. Natural history and epidemiology of Porphyromonas gingivalis, p. 3-31. In H. Shah, D. Mayrand, and R. J. Genco (ed.), Biology of the species Porphyromonas gingivalis. CRC Press, Boca Raton, Fla.
23. Meier, A., D. H. Persing, M. Finken, and E. C. Böttger. 1993. Elimination of contaminating DNA with polymerase chain reaction reagents: implications for a general approach to detection of uncultured pathogens. J. Clin. Microbiol. 31:646-652.
24. Moore, W. E. C., and L. V. H. Moore. 1994. The bacteria of periodontal diseases. Periodontology 2000 5:66-77.
25. Page, R. C., G. C. Armitage, T. A. DeRouen, R. J. Genco, P. Hujoel, M. K. Jeffcoat, K. S. Kornman, and R. C. Williams. 1995. Design and conduct of clinical trials of products designed for the prevention, diagnosis, and therapy of periodontitis. American Academy of Periodontology, Chicago.
26. Prince, A. M., and L. Andrus. 1992. PCR: how to kill unwanted DNA. BioTechniqucs 12:358-360.
27. Relman, D. A. 1993. Universal bacterial 16S rDNA amplification and sequencing, p. 489-495. In D. H. Persing, T. F. Smith, F. C. Tenover, and T. J. White (ed.), Diagnostic molecular microbiology: principles and applications. American Society for Microbiology, Washington, D.C.
28. Savitt, E. D., M. N. Strzempko, K. K. Vaccaro, W. J. Peros, and C. K. French. 1988. Comparison of cultural methods and DNA probe analyses for the detection of Actinobacillus actinomycetemcomitans, Bacteroides gingivalis, and Bacteroides intermedius in subgingival plaque samples. J. Periodontol. 59:431-438.
29. Socransky, S. S., C. Smith, L. Martin, B. J. Paster, F. E. Dewhirst, and A. E. Levin. 1994. "Checkerboard" DNA-DNA hybridization. BioTechniques 17: 788-792.
30. Tønjum, T., and R. Haas. 1993. Identification of Actinobacillus actinomycetemcomitans by leukotoxin gene-specific hybridization and polymerase chain reaction assays. J. Clin. Microbiol. 31:1856-1859.
31. van Winkelhoff, A. J., T. J. M. van Steenbergen, and J. de Graaff. 1993. Occurrence and association with disease, p. 33-42. In H. Shah, D. Mayrand, and R. J. Genco (ed.), Biology of the species Porphyromonas gingivalis. CRC Press, Boca Raton, Fla.
32. Wahlfors, J., J. H. Meurman, P. Vaisanen, P. Alakuijala, A. Korhonen, H. Torkko, and J. Janne. 1995. Simultaneous detection of Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis by a rapid PCR method. J. Dent. Res. 74:1796-1801.
33. Watanabe, K., and T. O. Frommel. 1993. Detection of Porphyromonas gingivalis in oral plaque samples by use of the polymerase chain reaction. J. Dent. Res. 72:1040-1044.
34. Wolff, L. F., D. M. Aeppli, B. L. Pihlstrom, L. Anderson, J. L. Stoltenberg, J. B. Osborn, N. A. Hardie, C. E. Shelburne, and G. E. Fisher. 1993. Natural distribution of 5 bacteria associated with periodontal disease. J. Clin. Periodontol. 20:699-706.
35. Wolff, L. F., G. Dahlèn, and D. M. Aeppli. 1994. Bacteria as risk markers for periodontitis. J. Periodontol. 64:498-510.
36. Zambon, J. J., and V. I. Haraszthy. 1995. The laboratory diagnosis of periodontal infections. Periodontology 2000 7:69-82.