In vivo RNA interference in oyster -vasa silencing inhibits germ cell development

FEBS Journal

Volumn 276, Issue 9, 2009, Pages 2566-2573

  Fabioux, Caroline ^a,b   Corporeau, Charlotte ^b,c   Quillien, Virgile ^b,c   Favrel, Pascal ^b,c   Huvet, Arnaud ^b,c  

^a UNIVERSITÉ DE BRETAGNE OCCIDENTALE   (France)

^b IFREMER   (France)

^c Biology and Innovative Therapeutics for Ovarian Cancers group   (France)

Author keywords

Crassostrea gigas; Germline; Marine bivalve; RNAi; Vasa

Indexed keywords

DOUBLE STRANDED RNA; GENE PRODUCT; MESSENGER RNA; PROTEIN OYVLG; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BIVALVE; CELL MATURATION; CELL PROLIFERATION; CONTROLLED STUDY; FEMALE; GENE; GENE EXPRESSION; GENE FUNCTION; GENE SILENCING; GENE TARGETING; GERM CELL; GONAD; IN VIVO STUDY; MALE; MEIOSIS; MITOSIS; NONHUMAN; NUCLEOTIDE SEQUENCE; OYSTER; OYVLG GENE; PHENOTYPE; PRIORITY JOURNAL; RNA INTERFERENCE;

BIVALVIA; CRASSOSTREA GIGAS; MOLLUSCA; OSTREIDAE;

EID: 64149105942     PISSN: 1742464X     EISSN: 17424658     Source Type: Journal    
DOI: 10.1111/j.1742-4658.2009.06982.x     Document Type: Article

References (29)

1. FAO (2004) The State of World Fisheries and Aquaculture. FAO Fisheries Department, part 1, 65.
2. Saavedra C Bachère E (2006) Bivalve genomics. Aquaculture 256, 1 14.
3. Jenny MJ, Chapman RW, Mancia A, Chen YA, McKillen DJ, Trent H, Lang P, Escoubas J-M, Bachere E, Boulo V et al. (2007) Characterization of a cDNA microarray for Crassostrea virginica and C. gigas. Mar Biotechnol 9, 577 591.
4. Elbashir SM, Harborth J, Lendeckel W, Yalcin A, Weber K Tuschl T (2001) Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature 411, 494 498.
5. Fire A, Xu S, Montgomery MK, Kostas SA, Driver SE Mello CC (1998) Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 391, 806 811.
6. Hannon GJ (2002) RNA interference. Nature 418, 244 251.
7. Meister G Tuschl T (2004) Mechanisms of gene silencing by double-stranded RNA. Nature 431, 343 349.
8. Berns K et al. (2004) A large-scale RNAi screen in human cells identifies new components of the p53 pathway. Nature 428, 431 437.
9. Xia H, Mao Q, Paulson HL Davidson BL (2002) siRNA-mediated gene silencing in vitro and in vivo. Nat Biotechnol 20, 1006 1010.
10. Robalino J, Browdy CL, Prior S, Metz A, Parnell P, Gross P Warr G (2004) Induction of antiviral immunity by double-stranded RNA in a marine invertebrate. J Virol 78, 10442 10448.
11. Dash PK, Tiwari M, Santhosh SR, Parida M Lakshmana Rao PV (2008) RNA interference mediated inhibition of Chikungunya virus replication in mammalian cells. Biochem Biophys Res Commun 376, 718 722.
12. Van Diepen MT, Spencer GE, Van Minnen J, Gouwenberg Y, Bouwman J, Smit AB Van Kesteren RE (2005) The molluscan RING-finger protein L-TRIM is essential for neuronal outgrowth. Mol Cell Neurosci 29, 74 81.
13. Grimaldi A, Tettamanti G, Rinaldi L, Brivio MF, Castellani D de Eguileor M (2004) Muscle differentiation in tentacles of Sepia officinalis (Mollusca) is regulated by muscle regulatory factors (MRF) related proteins. Dev Growth Differ 46, 83 95.
14. Kuznicki KA, Smith PA, Leung-Chiu WM, Estevez AO, Scott HC Bennett KL (2000) Combinatorial RNA interference indicates GLH-4 can compensate for GLH-1; these two P granule components are critical for fertility in C. elegans. Development 127, 2907 2916.
15. Lasko F Ashburner M (1988) The product of the Drosophila gene vasa is very similar to eucaryotic initiation factor-4A. Nature 335, 611 617.
16. Fabioux C, Huvet A, Lelong C, Robert R, Pouvreau S, Daniel JY, Mingant C Le Pennec M (2004) Oyster vasa-like gene as a marker of the germline cell development in Crassostrea gigas. Biochem Biophys Res Commun 320, 592 598.
17. Fabioux C, Pouvreau S, Le Roux F Huvet A (2004) The oyster vasa-like gene: a specific marker of the germline in Crassostrea gigas. Biochem Biophys Res Commun 315, 897 904.
18. Knaut H, Pelegri F, Bohmann K, Schwarz H Nuesslein-Volhard C (2000) Zebrafish vasa RNA but not its protein is a component of the germ plasm and segregates asymmetrically before germline specification. J Cell Biol 149, 875 888.
19. Echeverri CJ et al. (2006) Minimizing the risk of reporting false positives in large-scale RNAi screens. Nat Methods 3, 777 779.
20. Tanaka SS, Toyooka Y, Akasu R, Katoh-Fukui Y, Nakahara Y, Suzuki R, Yokoyama M Noce T (2000) The mouse homolog of Drosophila Vasa is required for the development of male germ cells. Gene Dev 14, 841 853.
21. Styhler S, Nakamura A, Swan A, Suter B Lasko P (1998) vasa is required for GURKEN accumulation in the oocyte, and is involved in oocyte differentiation and germline cyst development. Development 125, 1569 1578.
22. Saleh M-C, van Rij RP, Hekele A, Gillis A, Foley E, O'Farrell PH Andino R (2006) The endocytic pathway mediates cell entry of dsRNA to induce RNAi silencing. Nat Cell Biol 8, 793 802.
23. Jiang Y, Loker ES Zhang S-M (2006) In vivo and in vitro knockdown of FREP2 gene expression in the snail Biomphalaria glabrata using RNA interference. Dev Comp Immunol 30, 855 866.
24. Amdam GV, Simoes ZL, Guidugli KR, Norberg K Omholt SW (2003) Disruption of vitellogenin gene function in adult honeybees by intra-abdominal injection of double-stranded RNA. BMC Biotechnol 3, 1, doi :.
25. Sijen T, Fleenor J, Simmer F, Thijssen L, Parrish S, Timmons L, Plasterk R Fire A (2001) On the role of RNA amplification in dsRNA-triggered gene silencing. Cell 107, 465 476.
26. Agrawal N, Dasaradhi PVN, Mohmmed A, Malhotra P, Bhatnagar RK Mukherjee SK (2003) RNA interference: biology, mechanism, and applications. Microbiol Mol Biol Rev 67, 657 685.
27. Fleury E, Fabioux C, Lelong C, Favrel P Huvet A (2008) Characterization of a gonad-specific transforming growth factor-[beta] superfamily member differentially expressed during the reproductive cycle of the oyster Crassostrea gigas. Gene 410, 187 196.
28. Fabioux C, Huvet A, Le Souchu P, Le Pennec M Pouvreau S (2005) Temperature and photoperiod drive Crassostrea gigas reproductive internal clock. Aquaculture 250, 458 470.
29. Corporeau C Auffret M (2003) In situ hybridisation for flow cytometry: a molecular method for monitoring stress-gene expression in hemolymph cells of oysters. Aquat Toxicol 64, 427 435.