Structure and Function of the Macrolide Biosensor Protein, MphR(A), with and without Erythromycin

Journal of Molecular Biology

Volumn 387, Issue 5, 2009, Pages 1250-1260

  Zheng, Jianting ^a   Sagar, Vatsala ^a,b   Smolinsky, Adam ^a   Bourke, Chase ^a   LaRonde LeBlanc, Nicole ^a,b   Cropp, T Ashton ^a  

^a Bldg 142   (United States)

^b UNIVERSITY OF MARYLAND   (United States)

Author keywords

biosensor; erythromycin; macrolide antibiotic; repressor; Streptomyces

Indexed keywords

AZITHROMYCIN; ERYTHROMYCIN; JOSAMYCIN; MACROLIDE; METHYMYCIN;

ARTICLE; BIOSENSOR; BIOSYNTHESIS; CRYSTAL STRUCTURE; DNA BINDING; GENE REPRESSION; LIGAND BINDING; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN STRUCTURE; REGULATORY SEQUENCE;

STREPTOMYCES;

EID: 63449089393     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jmb.2009.02.058     Document Type: Article

References (34)

1. Weisblum B. Erythromycin resistance by ribosome modification. Antimicrob. Agents Chemother. 39 (1995) 577-585
2. Ross J.I., Eady E.A., Cove J.H., Cunliffe W.J., Baumberg S., and Wootton J.C. Inducible erythromycin resistance in staphylococci is encoded by a member of the ATP-binding transport super-gene family. Mol. Microbiol. 4 (1990) 1207-1214
3. Leclercq R., and Courvalin P. Intrinsic and unusual resistance to macrolide, lincosamide, and streptogramin antibiotics in bacteria. Antimicrob. Agents Chemother. 35 (1991) 1273-1276
4. Noguchi N., Emura A., Matsuyama H., O'Hara K., Sasatsu M., and Kono M. Nucleotide sequence and characterization of erythromycin resistance determinant that encodes macrolide 2′-phosphotransferase I in Escherichia coli. Antimicrob. Agents Chemother. 39 (1995) 2359-2363
5. Noguchi N., Takada K., Katayama J., Emura A., and Sasatsu M. Regulation of transcription of the mph(A) gene for macrolide 2′-phosphotransferase I in Escherichia coli: characterization of the regulatory gene mphR(A). J. Bacteriol. 182 (2000) 5052-5058
6. Szczepanowski R., Krahn I., Bohn N., Puhler A., and Schluter A. Novel macrolide resistance module carried by the IncP-1beta resistance plasmid pRSB111, isolated from a wastewater treatment plant. Antimicrob. Agents Chemother. 51 (2007) 673-678
7. Szczepanowski R., Krahn I., Linke B., Goesmann A., Puhler A., and Schluter A. Antibiotic multiresistance plasmid pRSB101 isolated from a wastewater treatment plant is related to plasmids residing in phytopathogenic bacteria and carries eight different resistance determinants including a multidrug transport system. Microbiology 150 (2004) 3613-3630
8. Ramos J.L., Martinez-Bueno M., Molina-Henares A.J., Teran W., Watanabe K., Zhang X., et al. The TetR family of transcriptional repressors. Microbiol. Mol. Biol. Rev. 69 (2005) 326-356
9. Weber W., Fux C., Daoud-El Baba M., Keller B., Weber C.C., Kramer B.P., et al. Macrolide-based transgene control in mammalian cells and mice. Nat. Biotechnol. 20 (2002) 901-907
10. Kramer B.P., Viretta A.U., Daoud-El Baba M., Aubel D., Weber W., and Fussenegger M. An engineered epigenetic transgene switch in mammalian cells. Nat. Biotechnol. 22 (2004) 867-870
11. Link N., Weber W., and Fussenegger M. A novel generic dipstick-based technology for rapid and precise detection of tetracycline, streptogramin and macrolide antibiotics in food samples. J. Biotechnol. 128 (2007) 668-680
12. Weber C.C., Link N., Fux C., Zisch A.H., Weber W., and Fussenegger M. Broad-spectrum protein biosensors for class-specific detection of antibiotics. Biotechnol. Bioeng. 89 (2005) 9-17
13. Mohrle V., Stadler M., and Eberz G. Biosensor-guided screening for macrolides. Anal. Bioanal. Chem. 388 (2007) 1117-1125
14. Hinrichs W., Kisker C., Duvel M., Muller A., Tovar K., Hillen W., and Saenger W. Structure of the Tet repressor-tetracycline complex and regulation of antibiotic resistance. Science 264 (1994) 418-420
15. Dover L.G., Corsino P.E., Daniels I.R., Cocklin S.L., Tatituri V., Besra G.S., and Futterer K. Crystal structure of the TetR/CamR family repressor Mycobacterium tuberculosis EthR implicated in ethionamide resistance. J. Mol. Biol. 340 (2004) 1095-1105
16. Schumacher M.A., Miller M.C., Grkovic S., Brown M.H., Skurray R.A., and Brennan R.G. Structural mechanisms of QacR induction and multidrug recognition. Science 294 (2001) 2158-2163
17. Krissinel E., and Henrick K. Secondary-Structure Matching (SSM), a new tool for fast protein structure alignment in three dimensions. Acta Crystallogr., Sect. D: Biol. Crystallogr. 60 (2004) 2256-2268
18. Jeng W.Y., Ko T.P., Liu C.I., Guo R.T., Liu C.L., Shr H.L., and Wang A.H. Crystal structure of IcaR, a repressor of the TetR family implicated in biofilm formation in Staphylococcus epidermidis. Nucleic Acids Res. 36 (2008) 1567-1577
19. Miller J.H. Experiments in Molecular Genetics (1972), Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
20. Willems A.R., Tahlan K., Taguchi T., Zhang K., Lee Z.Z., Ichinose K., et al. Crystal structures of the Streptomyces coelicolor TetR-like protein ActR alone and in complex with actinorhodin or the actinorhodin biosynthetic precursor (S)-DNPA. J. Mol. Biol. 376 (2008) 1377-1387
21. Anderson J.C., Voigt C.A., and Arkin A.P. Environmental signal integration by a modular AND gate. Mol. Syst. Biol. 3 (2007) 133
22. Sayut D.J., Kambam P.K., and Sun L. Engineering and applications of genetic circuits. Mol. BioSyst. 3 (2007) 835-840
23. Resch M., Striegl H., Henssler E.M., Sevvana M., Egerer-Sieber C., Schiltz E., et al. A protein functional leap: how a single mutation reverses the function of the transcription regulator TetR. Nucleic Acids Res. 36 (2008) 4390-4401
24. McDaniel R., Thamchaipenet A., Gustafsson C., Fu H., Betlach M., and Ashley G. Multiple genetic modifications of the erythromycin polyketide synthase to produce a library of novel "unnatural" natural products. Proc. Natl Acad. Sci. USA 96 (1999) 1846-1851
25. Yoon Y.J., Beck B.J., Kim B.S., Kang H.Y., Reynolds K.A., and Sherman D.H. Generation of multiple bioactive macrolides by hybrid modular polyketide synthases in Streptomyces venezuelae. Chem. Biol. 9 (2002) 203-214
26. Rowe C.J., Bohm I.U., Thomas I.P., Wilkinson B., Rudd B.A., Foster G., et al. Engineering a polyketide with a longer chain by insertion of an extra module into the erythromycin-producing polyketide synthase. Chem. Biol. 8 (2001) 475-485
27. Kim B.S., Sherman D.H., and Reynolds K.A. An efficient method for creation and functional analysis of libraries of hybrid type I polyketide synthases. Protein Eng. Des. Sel. 17 (2004) 277-284
28. Zhang Y.X., Perry K., Vinci V.A., Powell K., Stemmer W.P., and del Cardayre S.B. Genome shuffling leads to rapid phenotypic improvement in bacteria. Nature 415 (2002) 644-646
29. Otwinoswski Z., and Minor W. Processing of X-ray diffraction data collected in oscillation mode. Methods Enzymol. 276 (1997) 307-326
30. Vonrhein C., Blanc E., Roversi P., and Bricogne G. Automated structure solution with autoSHARP. Methods Mol. Biol. 364 (2007) 215-230
31. Emsley P., and Cowtan K. Coot: model-building tools for molecular graphics. Acta Crystallogr., Sect. D: Biol. Crystallogr. 60 (2004) 2126-2132
32. Murshudov G.N., Vagin A.A., and Dodson E.J. Refinement of macromolecular structures by the maximum-likelihood method. Acta Crystallogr., Sect. D: Biol. Crystallogr. 53 (1997) 240-255
33. Winn M.D., Isupov M.N., and Murshudov G.N. Use of TLS parameters to model anisotropic displacements in macromolecular refinement. Acta Crystallogr., Sect. D: Biol. Crystallogr. 57 (2001) 122-133
34. Kleywegt G.J., and Jones T.A. Detection, delineation, measurement and display of cavities in macromolecular structures. Acta Crystallogr., Sect. D: Biol. Crystallogr. 50 (1994) 178-185