Traditional GFP-type cyclization and unexpected fragmentation site in a purple chromoprotein from Anemonia sulcata, asFP595

Biochemistry

Volumn 43, Issue 42, 2004, Pages 13598-13603

  Zagranichny, Vasily E ^a   Rudenko, Natalia V ^a   Gorokhovatsky, Andrey Yu ^a   Zakharov, Mikhail V ^a   Balashova, Tamara A ^a   Arseniev, Alexander S ^a  

^a SHEMYAKIN OVCHINNIKOV INSTITUTE OF BIOORGANIC CHEMISTRY   (Russian Federation)

Author keywords

[No Author keywords available]

Indexed keywords

ADSORPTION; BIOCHEMISTRY; CHROMOPHORES; CLONING; ENZYME KINETICS; HYDROLYSIS; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY;

CHROMOPROTEINS; EMISSION SPECTRA; GREEN FLUORESCENT PROTEINS (GFP); PROTEOLYSIS;

PROTEINS;

GREEN FLUORESCENT PROTEIN; PURPLE CHROMOPROTEIN ASFP595; UNCLASSIFIED DRUG; YELLOW FLUORESCENT PROTEIN;

ARTICLE; CARBOXY TERMINAL SEQUENCE; CHROMATOPHORE; CYCLIZATION; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN FAMILY; PROTEIN PURIFICATION; SEA ANEMONE; SEQUENCE HOMOLOGY;

ACTINIARIA; ANEMONE; ANEMONIA; ANEMONIA SULCATA;

EID: 6344289261     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi0488247     Document Type: Article

References (26)

1. Tsien, R. Y. (1998) The green fluorescent protein, Annu. Rev. Biochem. 67, 509-544.
2. Matz, M. V., Fradkov, A. F., Labas, Y. A., Savitsky, A. P., Zaraisky, A. G., Markelov, M. L., and Lukyanov, S. A. (1999) Fluorescent proteins from nonbioluminescent Anthozoa species, Nat. Biotechnol. 17, 969-973.
3. Lukyanov, K. A., Fradkov, A. F., Gurskaya, N. G., Matz, M. V., Labas, Y. A., Savitsky, A. P., Markelov, M. L., Zaraisky, A. G., Zhao, X., Fang, Y., Tan, W., and Lukyanov, S. A.(2000) Natural animal coloration can be determined by a nonfluorescent green fluorescent protein homolog, J. Biol. Chem. 275, 25879-25882.
4. Prescott, M., Ling, M., Beddoe, T., Oakley, A. J., and Dove, S. (2003) The 2.2 Å crystal structure of a pocilloporin pigment reveals a nonplanar chromophore conformation, Structure (London) 11, 275-284.
5. Petersen, J., Wilmann, P. G., Beddoe, T., Oakley, A. J., Devenish, R. J., Prescott, M., and Rossjohn, J. (2003) The 2.0 Å crystal structure of eqFP611, a far-red fluorescent protein from the sea anemone Entacmaea quadricolor, J. Biol. Chem. 278, 44626-44631.
6. Gurskaya, N. G., Fradkov, A. F., Terskikh, A., Matz, M. V., Labas, Yu. A., Martynov, V. I., Yanushevich, Yu. G., Lukyanov, K. A., and Lukyanov, S. A. (2001) GFP-like chromoproteins as a source of far-red fluorescent proteins, FEBS Lett. 507, 16-20.
7. Verkhusha, V. V., and Lukyanov. K. A. (2004) The molecular properties and applications of Anthozoa fluorescent proteins and chromoproteins, Nat. Biotechnol. 22, 289-296.
8. Labas, Yu. A., Gurskaya, N. G., Yanushevich, Yu. G., Fradkov, A. F., Lukyanov, K. A., Lukyanov, S. A., and Matz, M. V. (2002) Diversity and evolution of the green fluorescent protein family, Proc. Natl. Acad. Sci. U.S.A. 99, 4256-4261.
9. Chudakov, D. M., Feofanov, A. V., Mudrik, N. N., Lukyanov, S., and Lukyanov, K. A. (2003) Chromophore environment provides clue to "kindling fluorescent protein" riddle, J. Biol. Chem. 278, 7215-7219.
10. Chudakov, D. M., Belousov, V. V., Zaraisky, A. G., Novoselov, V. V., Staroverov, D. B., Zorov, D. B., Lukyanov, S., and Lukyanov, K. A. (2003) Kindling fluorescent proteins for precise in vivo photolabeling, Nat. Biotechnol. 21, 191-194.
11. Remington, S. J. (2002) Negotiating the speed bumps to fluorescence, Nat. Biotechnol. 20, 28-29.
12. Ormö, M., Cubitt, A. B., Kallio, K., Gross, L. A., Tsien, R. Y., and Remington, S. J. (1996) Crystal structure of the Aequorea victoria green fluorescent protein, Science 273, 1392-1395.
13. Wall, M. A., Sokolich, M., and Ranganathan, R. (2000) The structural basis for red fluorescence in the tetrameric GFP homolog DsRed, Nat. Struct. Biol. 7, 1133-1138.
14. Yarbrough, D., Wachter, R. M., Kallio, K., Matz, M. V., and Remington, S. J. (2001) Refined crystal structure of DsRed, a red fluorescent protein from coral, at 2.0-Å resolution, Proc. Natl. Acad. Sci. U.S.A. 98, 462-467.
15. Martynov, V. I., Maksimov, B. I., Martynova, N. Y., Pakhomov, A. A., Gurskaya, N. G., and Lukyanov, S. A. (2003) A purple-blue chromoprotein from Goniopora tenuidens belongs to the DsRed subfamily of GFP-like proteins, J. Biol. Chem. 278, 46288-46292.
16. Zagranichny, V. E., Rudenko, N. V., Gorokhovatsky, A. Yu., Zakharov, M. V., Shenkarev, Z. O., Balashova, T. A., and Arseniev, A. S. (2004) zFP538, a yellow fluorescent protein from coral, belongs to the DsRed subfamily of GFP-like proteins but possesses the unexpected site of fragmentation, Biochemistry 43, 4764-4772.
17. Mizuno, H., Mal, T. K., Tong, K. I., Ando, R., Furuta, T., Ikura, M., and Miyawaki, A. (2003) Photo-induced peptide cleavage in the green-to-red conversion of a fluorescent protein, Mol. Cell 12, 1051-1058.
18. Martynov, V. I., Savitsky, A. P., Martynova, N. Y., Savitsky, P. A., Lukyanov, K. A., and Lukyanov, S. A. (2001) Alternative cyclization in GFP-like protein family: The formation and structure of the chromophore of a purple chromoprotein from Anemonia sulcata, J. Biol. Chem. 276, 21012-21016.
19. Cody, C. W., Prasher, D. C., Westler, W. M., Prendergast, F. G., and Ward, W. W. (1993) Chemical structure of the hexapeptide chromophore of the Aequorea green-fluorescent protein, Biochemistry 32, 1212-1218.
20. Shimomura, O. (1979) Structure of the chromophore of Aequorea green fluorescent protein, FEBS Lett. 104, 220-222.
21. Niwa, M., Inouye, S., Hirano, T., Matsuno, T., Kojima, S., Kubota, M., Ohashi, M., and Tsuji, F. I. (1996) Chemical nature of the light emitter of the Aequorea green fluorescent protein, Proc. Natl. Acad. Sci. U.S.A. 93, 13617-13622.
22. Ward, W. W., Cody, C. W., Hart, R. C., and Cormier, M. J. (1980) Spectrophotometric identity of the energy transfer chromophores in Renilla and Aequorea green-fluorescent proteins, Photochem. Photobiol. 31, 611-615.
23. Gross, L. A., Baird, G. S., Hoffman, R. C., Baldridge, K. K., and Tsien, R. Y. (2000) The structure of the chromophore within DsRed, a red fluorescent protein from coral, Proc. Natl. Acad. Sci. U.S.A. 97, 11990-11995.
24. He, X., Bell, A. F., and Tonge, P. J. (2002) Synthesis and spectroscopic studies of model red fluorescent protein chromophores, Org. Lett. 4, 1523-1526.
25. Smith, P. A. S. (1965) The chemistry of open-chain organic nitrogen compounds, pp 291-341, W. A. Benjamin, Inc., New York, Amsterdam.
26. Heng Suen, Y., and Kagan, H. B. (1965) Iminomagnésiens. III. - Propriétés d'acylimines et acylénamines, Bull. Soc. Chim. Fr. 5, 1460-1463.