Photo-induced peptide cleavage in the green-to-red conversion of a fluorescent protein

Molecular Cell

Volumn 12, Issue 4, 2003, Pages 1051-1058

  Mizuno, Hideaki ^a   Mal, Tapas Kumar ^b   Tong, Kit I ^b   Ando, Ryoko ^a   Furuta, Toshiaki ^c   Ikura, Mitsuhiko ^b   Miyawaki, Atsushi ^a  

^a RIKEN BRAIN SCIENCE INSTITUTE   (Japan)

^b UNIVERSITY OF TORONTO   (Canada)

^c TOHO UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

2 [2 (5 IMIDAZOLYL)ETHENYL] 4 (4 HYDROXYBENZYLIDENE) 5 IMIDAZOLINONE; AMIDE; GLYCINE; GREEN FLUORESCENT PROTEIN; HISTIDINE; IMIDAZOLINE DERIVATIVE; KAEDE PROTEIN; NITROGEN; PEPTIDE; PROTEIN; TRIPEPTIDE; UNCLASSIFIED DRUG;

ARTICLE; BIODIVERSITY; CHEMICAL STRUCTURE; CHROMATOPHORE; CONTROLLED STUDY; GENETIC CODE; JELLYFISH; LIGHT ABSORPTION; NONHUMAN; PROTEIN BINDING; PROTEIN DEGRADATION; PROTEIN PROCESSING; ULTRAVIOLET IRRADIATION;

AEQUOREA; ANTHOZOA; SCYPHOZOA;

EID: 0242361312     PISSN: 10972765     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1097-2765(03)00393-9     Document Type: Article

References (23)

1. Ando R., Hama H., Yamamoto-Hino M., Mizuno H., Miyawaki A. An optical marker based on the UV-induced green-to-red photoconversion of a fluorescent protein. Proc. Natl. Acad. Sci. USA. 99:2002;12651-12656.
2. Baird G.S., Zacharias D.A., Tsien R.Y. Biochemistry, mutagenesis, and oligomerization of DsRed, a red fluorescent protein from coral. Proc. Natl. Acad. Sci. USA. 97:2000;11984-11989.
3. Bax A., Summer M.F. Proton and carbon-13 assignments from sensitivity-enhanced detection of heteronuclear multiple-bond connectivity by 2D multiple quantum NMR. J. Am. Chem. Soc. 108:1986;2093-2094.
4. Brejc K., Sixma T.K., Kitts P.A., Kain S.R., Tsien R.Y., Ormo M., Remington S.J. Structural basis for dual excitation and photoisomerization of the Aequorea victoria green fluorescent protein. Proc. Natl. Acad. Sci. USA. 94:1997;2306-2311.
5. Chattoraj M., King B.A., Bublitz G.U., Boxer S.G. Ultra-fast excited state dynamics in green fluorescent protein. multiple states and proton transfer Proc. Natl. Acad. Sci. USA. 93:1996;8362-8367.
6. Cody C.W., Prasher D.C., Westler W.M., Prendergast F.G., Ward W.W. Chemical structure of the hexapeptide chromophore of the Aequorea green-fluorescent protein. Biochemistry. 32:1993;1212-1218.
7. Gross L.A., Baird G.S., Hoffman R.C., Baldridge K.K., Tsien R.Y. The structure of the chromophore within DsRed, a red fluorescent protein from coral. Proc. Natl. Acad. Sci. USA. 97:2000;11990-11995.
8. Heim R., Prasher D.C., Tsien R.Y. Wavelength mutations and posttranslational autoxidation of green fluorescent protein. Proc. Natl. Acad. Sci. USA. 91:1994;12501-12504.
9. Lukyanov K.A., Fradkov A.F., Gurskaya N.G., Matz M.V., Labas Y.A., Savitsky A.P., Markelov M.L., Zaraisky A.G., Zhao X., Fang Y.et al. Natural animal coloration can be determined by a nonfluorescent green fluorescent protein homolog. J. Biol. Chem. 275:2000;25879-25882.
10. McAnaney T.B., Park E.S., Hanson G.T., Remington S.J., Boxer S.G. Green fluorescent protein variants as ratiometric dual emission pH sensors. 2. Excited-state dynamics. Biochemistry. 41:2002;15489-15494.
11. Martynov V.I., Savitsky A.P., Martynova N.Y., Savitsky P.A., Lukyanov K.A., Lukyanov S.A. Alternative cyclization in GFP-like proteins family. The formation and structure of the chromophore of a purple chromoprotein from Anemonia sulcata. J. Biol. Chem. 276:2001;21012-21016.
12. Matz M.V., Fradkov A.F., Labas Y.A., Savitsky A.P., Zaraisky A.G., Markelov M.L., Lukyanov S.A. Fluorescent proteins from nonbioluminescent Anthozoa species. Nat. Biotechnol. 17:1999;969-973.
13. Mizuno H., Sawano A., Eli P., Hama H., Miyawaki A. Red fluorescent protein from Discosoma as a fusion tag and a partner for fluorescence resonance energy transfer. Biochemistry. 40:2001;2502-2510.
14. Ormö M., Cubitt A.B., Kallio K., Gross L.A., Tsien R.Y., Remington S.J. Chemical structure of the hexapeptide chromophore of the Aequorea green-fluorescent protein. Science. 273:1996;1392-1395.
15. Prasher D.C., Eckenrode V.K., Ward W.W., Prendergast F.G., Cormier M.J. Primary structure of the Aequorea victoria green-fluorescent protein. Gene. 111:1992;229-233.
16. Reid B.G., Flynn G.C. Chromophore formation in green fluorescent protein. Biochemistry. 36:1997;6786-6791.
17. Shimomura O. Structure of the chromophore of Aequorea green fluorescent protein. FEBS Lett. 104:1979;220-222.
18. Tsien R.Y. The green fluorescent protein. Annu. Rev. Biochem. 67:1998;509-544.
19. Tozzini V., Nifosì R. Ab initio molecular dynamics of the green fluorescent protein (GFP) chromophore. an insight into the photoinduced dynamics of green fluorescent proteins J. Phys. Chem. B. 105:2001;5797-5803.
20. Wall M.A., Socolich M., Ranganathan R. The structural basis for red fluorescence in the tetrameric GFP homolog DsRed. Nat. Struct. Biol. 7:2000;1133-1138.
21. Weber W., Helms V., McCammon J.A., Langhoff P.W. Shedding light on the dark and weakly fluorescent states of green fluorescent proteins. Proc. Natl. Acad. Sci. USA. 96:1999;6177-6182.
22. Yang F., Moss L.G., Phillips G.N. Jr. The molecular structure of green fluorescent protein. Nat. Biotechnol. 14:1996;1246-1251.
23. Yarbrough D., Wachter R.M., Kallio K., Matz M.V., Remington S.J. Refined crystal structure of DsRed, a red fluorescent protein from coral, at 2.0-A resolution. Proc. Natl. Acad. Sci. USA. 98:2001;462-467.