Protocadherin FAT1 binds Ena/VASP proteins and is necessary for actin dynamics and cell polarization

EMBO Journal

Volumn 23, Issue 19, 2004, Pages 3769-3779

  Moeller, Marcus J ^a,b   Soofi, Abdulsalam ^b   Braun, Gerald S ^a   Li, Xiaodong ^b   Watzl, Garsten ^a   Kriz, Wilhelm ^a   Holzman, Lawrence B ^b  

^a UNIVERSITY OF HEIDELBERG   (Germany)

^b UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

Author keywords

Actin dynamics; Cellular migration; FAT1; Planar cell polarity; VASP

Indexed keywords

CADHERIN; FAT1 PROTEIN; UNCLASSIFIED DRUG; VASODILATOR STIMULATED PHOSPHOPROTEIN;

ARTICLE; CELL MIGRATION; CELL MOTILITY; CELL POLARITY; CYTOSKELETON; DROSOPHILA; EPITHELIUM CELL; FILOPODIUM; LAMELLIPODIUM; MAMMAL; MOLECULAR DYNAMICS; POLYMERIZATION; PRIORITY JOURNAL; PROTEIN BINDING; SIGNAL TRANSDUCTION;

ACTINS; ANIMALS; CADHERINS; CELL ADHESION MOLECULES; CELL MOVEMENT; CELL POLARITY; CYTOSKELETON; GENES, TUMOR SUPPRESSOR; HUMANS; IMMUNOGLOBULIN G; MICE; PSEUDOPODIA; RABBITS; RNA, SMALL INTERFERING; WOUND HEALING;

ENA; MAMMALIA;

EID: 6344276685     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.emboj.7600380     Document Type: Article

References (30)

1. Bear JE, Svitkina TM, Krause M, Schafer DA, Loureiro JJ, Strasser GA, Maly IV, Chaga OY, Cooper JA, Borisy GG, Gertler FB (2002) Antagonism between Ena/VASP proteins and actin filament capping regulates fibroblast motility. Cell 109: 509-521
2. Bershadsky AD, Futerman AH (1994) Disruption of the Golgi apparatus by brefeldin A blocks cell polarization and inhibits directed cell migration. Proc Natl Acad Sci USA 91: 5686-5689
3. Ciani L, Patel A, Alien ND, ffrench-Constant C (2003) Mice lacking the giant protocadherin mFAT1 exhibit renal slit junction abnormalities and a partially penetrant cyclopia and anophthalmia phenotype. Mol Cell Biol 23: 3575-3582
4. Cox B, Hadjantonakis AK, Collins JE, Megee AI (2000) Cloning and expression throughout mouse development of mfat1, a homologue of the Drosophila tumour suppressor gene fat. Dev Dyn 217: 233-240
5. Dunne J, Hanby AM, Poulsom R, Jones TA, Sheer D, Chin WG, Da SM, Zhao Q, Beverley PC, Owen MJ (1995) Molecular cloning and tissue expression of FAT, the human homologue of the Drosophila fat gene that is located on chromosome 4q34-q35 and encodes a putative adhesion molecule. Genomics 30: 207-223
6. Fradelizi J, Noireaux V, Plastino J, Menichi B, Louvard D, Sykes C, Golsteyn RM, Friederich E (2001) ActA and human zyxin harbour Arp2/3-independent actin-polymerization activity. Nat Cell Biol 3: 699-707
7. Hinz B, Alt W, Johnen C, Herzog V, Kaiser HW (1999) Quantifying lamella dynamics of cultured cells by SACED, a new computer-assisted motion analysis. Exp Cell Res 251: 234-243
8. Inoue T, Yaoita E, Kurihara H, Shimizu F, Sakai T, Kobayashi T, Ohshiro K, Kawachi H, Okada H, Suzuki H, Kihara I, Yamamoto T (2001) FAT is a component of glomerular slit diaphragms. Kidney Int 59: 1003-1012
9. Kaltschmidt JA, Lawrence N, Morel V, Balayo T, Fernandez BG, Pelissier A, Jacinto A, Martinez Arias A (2002) Planar polarity and actin dynamics in the epidermis of Drosophila. Nat Cell Biol 4: 937-944
10. Kamberov E, Makarova O, Roh M, Liu A, Karnak D, Straight S, Margolis B (2000) Molecular cloning and characterization of Pals, proteins associated with mLin-7. J Biol Chem 275: 11425-11431
11. Ma D, Yang CH, McNeill H, Simon MA, Axelrod JD (2003) Fidelity in planar cell polarity signalling. Nature 421: 543-547
12. Mahoney PA, Weber U, Onofrechuk P, Biessmann H, Bryantand PJ, Goodman CS (1991) The fat tumor suppressor gene in Drosophila encodes a novel member of the cadherin gene superfamily. Cell 67: 853-868
13. Makarova O, Kamberov E, Margolis B (2000) Generation of deletion and point mutations with one primer in a single cloning step. Biotechniques 29: 970-972
14. Mitsui K, Nakajima D, Ohara O, Nakayama M (2002) Mammalian fat3: a large protein that contains multiple cadherin and EGF-like motifs. Biochem Biophys Res Commun 290: 1260-1266
15. Moeller MJ, Sanden SK, Soofi A, Wiggins RC, Holzman LB (2002) Two gene fragments that direct podocyte-specific expression in transgenic mice. J Am Soc Nephrol 13: 1561-1567
16. Niebuhr K, Ebel F, Frank R, Reinhard M, Domann E, Carl UD, Walter U, Gertler FB, Wehland J, Chakraborty T (1997) A novel proline-rich motif present in ActA of Listeria monocytogenes and cytoskeletal proteins is the ligand for the EVH1 domain, a protein module present in the Ena/VASP family. EMBO J 16: 5433-5544
17. Nihalani D, Meyer D, Pajni S, Holzman LB (2001) Mixed lineage kinase-dependent JNK activation is governed by interactions of scaffold protein JIP with MAPK module components. EMBO J 20: 3447-3458
18. Nobes CD, Hall A (1999) Rho GTPases control polarity, protrusion, and adhesion during cell movement. J Cell Biol 144: 1235-1244
19. Paul CP, Good PD, Winer I, Engelke DR (2002) Effective expression of small interfering RNA in human cells. Nat Biotechnol 20: 505-508
20. Pistor S, Chakraborty T, Niebuhr K, Domann E, Wehland J (1994) The ActA protein of Listeria monocytogenes acts as a nucleator inducing reorganization of the actin cytoskeleton. EMBO J 13: 758-763
21. Ponassi M, Jacques TS, Ciani L, ffrench Constant C (1999) Expression of the rat homologue of the Drosophila fat tumour suppressor gene. Mech Dev 80: 207-212
22. Rawls AS, Guinto JB, Wolff T (2002) The cadherins fat and dachsous regulate dorsal/ventral signaling in the Drosophila eye. Curr Biol 12: 1021-1026
23. Rottner K, Behrendt B, Small JV, Wehland J (1999) VASP dynamics during lamellipodia protrusion. Nat Cell Biol 1: 321-322
24. Rubinson DA, Dillon CP, Kwiatkowski AV, Sievers C, Yang L, Kopinja J, Rooney DL, Ihrig MM, McManus MT, Gertler FB, Scott ML, Van Parijs L (2003) A lentivirus-based system to functionally silence genes in primary mammalian cells, stem cells and transgenic mice by RNA interference. Nat Genet 33: 401-406
25. Samarin S, Romero S, Kocks C, Didry D, Pantaloni D, Carlier MF (2003) How VASP enhances actin-based motility. J Cell Biol 163: 131-142
26. Tanoue T, Takeichi M (2004) Mammalian Fat1 cadherin regulates actin dynamics and cell-cell contact. J Cell Biol 165: 517-528
27. Vasioukhin V, Bauer C, Yin M, Fuchs E (2000) Directed actin polymerization is the driving force for epithelial cell-cell adhesion. Cell 100: 209-219
28. Welch MD, DePace AH, Verma S, Iwamatsu A, Mitchison TJ (1997) The human Arp2/3 complex is composed of evolutionarily conserved subunits and is localized to cellular regions of dynamic actin filament assembly. J Cell Biol 138: 375-384
29. Wu Q, Maniatis T (2000) Large exons encoding multiple ectodomains are a characteristic feature of protocadherin genes. Proc Natl Acad Sci USA 97: 3124-3129
30. Yang CH, Axelrod JD, Simon MA (2002) Regulation of Frizzled by fat-like cadherins during planar polarity signaling in the Drosophila compound eye. Cell 108: 675-688