Construction of a vector generating both siRNA and a fluorescent reporter: A siRNA study in cultured neurons

Molecules and Cells

Volumn 18, Issue 1, 2004, Pages 127-130

  Yoon, Seung Yoon ^a   Choi, Jung Eun ^a   Hwang, Onyou ^a   Hong, Hea Nam ^a   Lee, Heuiran ^a   Kim, Yoo Kyum ^a   Cho, Sung Woo ^a   Kim, Hyun ^b   Kim, Dong Hou ^a  

^a University of Ulsan College of Medicine   (South Korea)

^b Korea University College of Medicine   (South Korea)

Author keywords

Fluorescent reporter; Neuron; siRNA

Indexed keywords

BIM PROTEIN; DOUBLECORTIN; GREEN FLUORESCENT PROTEIN; RED FLUORESCENT PROTEIN; SMALL INTERFERING RNA;

ANIMAL CELL; ARTICLE; BRAIN CORTEX; BRAIN NERVE CELL; CONTROLLED STUDY; EMBRYO; EXPRESSION VECTOR; FLUORESCENCE MICROSCOPY; GENE EXPRESSION; GENE FUNCTION; GENE LOSS; GENE SILENCING; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; IMMUNOCYTOCHEMISTRY; IMMUNOREACTIVITY; NERVE CELL CULTURE; NONHUMAN; NUCLEOTIDE SEQUENCE; RAT; REPORTER GENE; RNA INTERFERENCE; RNA SEQUENCE;

ANIMALS; APOPTOSIS REGULATORY PROTEINS; CARRIER PROTEINS; CELLS, CULTURED; GENES, REPORTER; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; HUMANS; MEMBRANE PROTEINS; MICROTUBULE-ASSOCIATED PROTEINS; NEURONS; NEUROPEPTIDES; PROTO-ONCOGENE PROTEINS; RNA INTERFERENCE; RNA, SMALL INTERFERING;

EID: 6344230040     PISSN: 10168478     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (9)

1. Bai, J., Ramos, R. L., Ackman, J. B., Thomas, A, M., Lee, R. V., and Lo Turco, J. J. (2003) RNAi reveals doublecortin is required for radial migration in rat neocortex. Nat. Neurosci. 6, 1277-1283.
2. Brummelkamp, T. R., Bernards, R., and Agami, R. (2002) A system for stable expression of short interfering RNAs in mammalian cells. Science 296, 550-553.
3. Caplen, N. J., Parrish, S., Imani, F., Fire, A., and Morgan, R. A. (2001) Specific inhibition of gene expression by small double-stranded RNAs in invertebrate and vertebrate systems. Proc. Natl. Acad. Sci. USA 98, 9742-9747.
4. Cho, S. J., Jung, J. S., Jin, I., Jung, Y. W., Ko, B. H., Nam, K. S., and Park, I. K. (2002) Effects of 2,3,7,8-tetrachlorodibenzo-p-Dioxin on the expression of synapticproteins in dissociated rat cortical cells. Mol. Cells 14, 238-244.
5. Elbashir, S. M., Harborth, J., Lendeckel, W., Yalcin, A., Weber, K., and Tuschl, T. (2001) Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature 411, 494-498.
6. Hammond, S. M., Candy, A. A., and Hannon, G. J. (2001) Post-transcriptional gene silencing by double-stranded RNA. Nat. Rev. Genet. 2, 110-119.
7. Hutvágner, G. and Zamore, P. D. (2002) RNAi: nature abhors a double-strand. Curr. Opin. Genet. Dev. 12, 225-232.
8. Jacobsen, K. X. and Staines, W. A. (2004) Vibration enhancement of slide-mounted immunofluorescence staining, J. Neurosci. Methods (in press).
9. Krichevsky, A. M. and Kosik, K. S. (2002) RNAi functions in cultured mammalian neurons. Proc. Natl. Acad. Sci. USA 99, 11926-11929.