Transfection improvements of fish cell lines by using deacylated polyethylenimine of selected molecular weights

Fish and Shellfish Immunology

Volumn 26, Issue 3, 2009, Pages 559-566

  Falco, A ^b   Encinas, P ^a   Carbajosa, S ^a   Cuesta, A ^a   Chaves Pozo, E ^a   Tafalla, C ^a   Estepa, A ^b   Coll, J M ^a  

^a INIA   (Spain)

^b MIGUEL HERNÁNDEZ UNIVERSITY   (Spain)

Author keywords

Deacylated polyethylenimine; Fish cell transfection; Glycoprotein; Molecular weight fractionation; PEI; Rhabdovirus; VHSV

Indexed keywords

EPITHELIOMA PAPULOSUM; ONCORHYNCHUS MYKISS; RHABDOVIRIDAE; VIRAL HEMORRHAGIC SEPTICEMIA VIRUS;

EID: 62749109029     PISSN: 10504648     EISSN: 10959947     Source Type: Journal    
DOI: 10.1016/j.fsi.2009.02.013     Document Type: Article

References (47)

1. Fijan N., Sulimanovic D., Bearzotti M., Muzinic D., Zwillenberg L.O.Z., Chilmonczyk S., et al. Some properties of the epithelioma papulosum cyprini (EPC) cell line from carp cyprinus carpio. Ann Virol (Inst Pasteur) 134 (1983) 207-220
2. Wolf F., and Quimby M.C. Established eurythermic line of fish cells in vitro. Science 135 (1962) 1065-1066
3. Montero J., Coll J., Sevilla N., Cuesta A., Bols N.C., and Tafalla C. Interleukin 8 and CK-6 chemokines specifically attract rainbow trout (Oncorhynchus mykiss) RTS11 monocyte-macrophage cells and have variable effects on their immune functions. Dev Comp Immunol 32 (2008) 1374-1384
4. Tafalla C., Aranguren R., Secombes C.J., Figueras A., and Novoa B. Cloning and analysis of expression of a gilthead sea bream (Sparus aurata) Mx cDNA. Fish Shellfish Immunol 16 (2004) 11-24
5. Tafalla C., Chico V., Perez L., Coll J.M., and Estepa A. In vitro and in vivo differential expression of rainbow trout (Oncorhynchus mykiss) Mx isoforms in response to viral haemorrhagic septicaemia virus (VHSV) G gene, poly I:C and VHSV. Fish Shellfish Immunol 23 (2007) 210-221
6. Hackett PB, Alvarez MC. The molecular genetics of transgenic fish. Recent advances in marine biotechnology. In: Fingerman M, Nagabhushanam, R, editors. Aquaculture, Part B Fishes, vol. 4; 2000. p. 77-145.
7. Rocha A., Ruiz S., Tafalla C., and Coll J.M. Conformation and fusion defective mutants in the hypothetical phospholipid-binding and fusion peptides of the protein G of viral haemorrhagic septicemia salmonid rhabdovirus. J Virol 78 (2004) 9115-9122
8. Rocha A., Ruiz S., Tafalla C., and Coll J.M. Characterisation of the syncytia formed by VHS salmonid rhabdovirus G-gene transfected cells. Vet Immunol Immunopathol 99 (2004) 143-152
9. Acosta F., Collet B., Lorenzen N., and Ellis A.E. Expression of the glycoprotein of viral haemorrhagic septicaemia virus (VHSV) on the surface of the fish cell line RTG-P1 induces type 1 interferon expression in neighbouring cells. Fish Shellfish Immunol (2006)
10. Fernandez-Alonso M., Alvarez F., Estepa A., Blasco R., and Coll J.M. A model to study fish DNA immersion vaccination by using the green fluorescent protein. J Fish Disease 22 (1999) 237-241
11. Fernandez-Alonso M., Rocha A., and Coll J.M. DNA vaccination by immersion and ultrasound to trout viral haemorrhagic septicaemia virus. Vaccine 19 (2001) 3067-3075
12. Lorenzen N., Lorenzen E., Einer-Jensen K., and LaPatra S.E. DNA vaccines as a tool for analysing the protective immune response against rhabdoviruses in rainbow trout. Fish Shellfish Immunol 12 (2002) 439-453
13. Sommerset I., Krossoy B., Biering E., and Frost P. Vaccines for fish in aquaculture. Expert Rev Vaccines 4 (2005) 89-101
14. Kurath G. Biotechnology and DNA vaccines for aquatic animals. Rev Sci Technol 27 (2008) 175-196
15. Collet B., Boudinot P., Benmansour A., and Secombes C.J. An Mx1 promoter-reporter system to study interferon pathways in rainbow trout. Dev Comp Immunol 28 (2004) 793-801
16. Ruiz S., Tafalla C., Cuesta A., Estepa A., and Coll J.M. In vitro search for alternative promoters to the human immediate early-cytomegalovirus (IE-CMV) to express the G gene of viral haemorrhagic septicemia virus (VHSV) in fish epithelial cells. Vaccine 26 (2008) 6620-6629
17. Bremont M. Reverse genetics on fish rhabdoviruses: tools to study the pathogenesis of fish rhabdoviruses. Current topics in microbiology and immunology. World of rhabdoviruses. (2005) 119-141
18. Ruiz S, Schyth BD, Tafalla C, Estepa A, Lorenzen E, Coll JM. New tools to study RNA interference to fish viruses: cell lines permanently expressing siRNAs targeting the polymerase of viral haemorrhagic septicemia virus. Antivir Res, in press.
19. Utke K., Bergmann S., Lorenzen N., Kollner B., Ototake M., and Fischer U. Cell-mediated cytotoxicity in rainbow trout, Oncorhynchus mykiss, infected with viral haemorrhagic septicaemia virus. Fish Shellfish Immunol 22 (2007) 182-196
20. Utke K., Kock H., Schuetze H., Bergmann S.M., Lorenzen N., Einer-Jensen K., et al. Cell-mediated immune responses in rainbow trout after DNA immunization against the viral hemorrhagic septicemia virus. Dev Comp Immunol 32 (2007) 239-252
21. Bearzotti M., Perrot E., Michard-Vanhee C., Jolivet G., Attal J., Theron M.C., et al. Gene expression following transfection of fish cells. J Biotechnol 26 (1992) 315-325
22. Lopez A., Fernandez-Alonso M., Rocha A., Estepa A., and Coll J.M. Transfection of epithelioma cyprini (EPC) carp cells. Biotechnol Lett 23 (2001) 481-487
23. Rocha A., Ruiz S., Estepa A., and Coll J.M. Fish as biofactories: inducible genetic systems and gene targeting. Spanish J Agric Res 1 (2003) 3-11
24. Trobridge G.D., Chiou P.P., Kim C.H., and Leong J.C. Induction of the Mx protein of rainbow trout Oncorhynchus mykiss in vitro and in vivo with poly I:C dsRNA and infectious hematopoietic necrosis virus. Dis Aquat Org 30 (1997) 91-98
25. Carpentier E., Paris S., Kamen A.A., and Durocher Y. Limiting factors governing protein expression following polyethylenimine-mediated gene transfer in HEK293-EBNA1 cells. J Biotechnol 128 (2007) 268-280
26. Li D., Tang G.P., Li J.Z., Kong Y., Huang H.L., Min L.J., et al. Dual-targeting non-viral vector based on polyethylenimine improves gene transfer efficiency. J Biomater Sci Polym Ed 18 (2007) 545-560
27. Xiong M.P., Forrest M.L., Karls A.L., and Kwon G.S. Biotin-triggered release of poly(ethylene glycol)-avidin from biotinylated polyethylenimine enhances in vitro gene expression. Bioconjug Chem 18 (2007) 746-753
28. Breunig M., Lungwitz U., Liebl R., Klar J., Obermayer B., Blunk T., et al. Mechanistic insights into linear polyethylenimine-mediated gene transfer. Biochim Biophys Acta 1770 (2007) 196-205
29. Zhou J., Yockman J.W., Kim S.W., and Kern S.E. Intracellular kinetics of non-viral gene delivery using polyethylenimine carriers. Pharm Res 24 (2007) 1079-1087
30. Huh S.H., Do H.J., Lim H.Y., Kim D.K., Choi S.J., Song H., et al. Optimization of 25 kDa linear polyethylenimine for efficient gene delivery. Biologicals 35 (2007) 165-171
31. Talsma S.S., Babensee J.E., Murthy N., and Williams I.R. Development and in vitro validation of a targeted delivery vehicle for DNA vaccines. J Control Release 112 (2006) 271-279
32. Thomas M., Lu J.J., Zhang C., Chen J., and Klibanov A.M. Identification of novel superior polycationic vectors for gene delivery by high-throughput synthesis and screening of a combinatorial library. Pharm Res 24 (2007) 1564-1571
33. Thomas M., Lu J.J., Ge Q., Zhang C., Chen J., and Klibanov A.M. Full deacylation of polyethylenimine dramatically boosts its gene delivery efficiency and specificity to mouse lung. Proc Natl Acad Sci U S A 102 (2005) 5679-5684
34. Akinc A., Thomas M., Klibanov A.M., and Langer R. Exploring polyethylenimine-mediated DNA transfection and the proton sponge hypothesis. J Gene Med 7 (2005) 657-663
35. Salonius K., Simard N., Harland R., and Ulmer J.B. The road to licensure of a DNA vaccine. Curr Opin Investig Drug 8 (2007) 635-641
36. Tafalla C., Estepa A., and Coll J.M. New vaccination strategies for the improvement of DNA vaccines against virus in fish. In: Schwartz S.H. (Ed). Aquaculture research trends (2008), Nova Publishers 213-235 [chapter 6]
37. Moav B., Liu Z., Groll Y., and Hackett P.B. Selection of promoters for gene transfer into fish. Mol Marine Biol Biotechnol 1 (1992) 338-345
38. Rocha A., Ruiz S., and Coll J.M. Improvement of transfection efficiency of epithelioma papulosum cyprini carp cells by modification of their cell cycle and using an optimal promoter. Marine Biotechnol 6 (2005) 401-410
39. LeBerre M., De Kinkelin P., and Metzger A. Identification sérologique des rhabdovirus des salmonidés. Bull Off Int Epizoot 87 (1977) 391-393
40. Bearzotti M., Monnier A.F., Vende P., Grosclaude J., De Kinkelin P., and Benmansour A. The glycoprotein of viral hemorrhagic septicemia virus (VHSV): antigenicity and role in virulence. Vet Res 26 (1995) 413-422
41. Gaudin Y., De Kinkelin P., and Benmansour A. Mutations in the glycoprotein of viral haemorrhagic septicaemia virus that affect virulence for fish and the pH threshold for membrane fusion. J Gen Virol 80 (1999) 1221-1229
42. Estepa A., and Coll J.M. Pepscan mapping and fusion related properties of the major phosphatidylserine-binding domain of the glycoprotein of viral hemorrhagic septicemia virus, a salmonid rhabdovirus. Virology 216 (1996) 60-70
43. Lorenzo G.A., Estepa A., Chilmonczyk S., and Coll J.M. Mapping of the G-regions and N-regions of Viral Hemorrhagic Septicemia Virus (VHSV) inducing lymphoproliferation by pepscan. Vet Res 26 (1995) 521-525
44. Rocha A., Ruiz S., Tafalla C., and Coll J.M. Characterisation of the syncytia formed by VHS salmonid rhabdovirus G gene transfected cells. Vet Immunol Immunopathol 99 (2004) 143-152
45. Falco A., Brocal I., Perez L., Coll J.M., Estepa A., and Tafalla C. In vivo modulation of the rainbow trout (Oncorhynchus mykiss) immune response by the human alpha defensin 1, HNP1. Fish Shellfish Immunol 24 (2008) 102-112
46. Mas V., Rocha A., Perez L., Coll J.M., and Estepa A. Reversible inhibition of spreading of in vitro infection and imbalance of viral protein accumulation at low pH in Viral Haemorrhagic Septicaemia Rhabdovirus (VHSV), a salmonid rhabdovirus. J Virol 78 (2004) 1936-1944
47. Rocha A., Ruiz S., and Coll J.M. Improvement of DNA transfection with cationic liposomes. J Physiol Biochem 58 (2002) 45-56