Protein kinase-a affects sorting and conformation of the sodium-dependent glucose co-transporter SGLT1

Journal of Cellular Biochemistry

Volumn 106, Issue 3, 2009, Pages 444-452

  Subramanian, Supriya ^a   Gliiz, Petra ^a   Kipp, Helmut ^b   Kinne, Rolf K H ^a   Castaneda, Francisco ^a  

^a MAX PLANCK INSTITUTE OF MOLECULAR PHYSIOLOGY   (Germany)

^b UNIVERSITY OF WÜRZBURG   (Germany)

Author keywords

methyl d glucopyranoside; ACTIVE TRANSPORT; KINETIC STUDIES; PROTEIN K1NASE A; SGLT1; STERN VOEMER QUENCHING CONSTANT

Indexed keywords

8 BROMO CYCLIC AMP; ADENOSINE TRIPHOSPHATE; ALPHA METHYLGLUCOSIDE; CYCLIC AMP DEPENDENT PROTEIN KINASE; CYTOCHALASIN D; FORSKOLIN; GLUCOSE; PHOSPHORUS 32; SODIUM GLUCOSE COTRANSPORTER 1; TRYPTOPHAN; METHYLGLUCOSIDE;

ANIMAL CELL; ARTICLE; BIOTINYLATION; CELL MEMBRANE; CONTROLLED STUDY; ENZYME PHOSPHORYLATION; EPITHELIUM CELL; GLUCOSE TRANSPORT; IMMUNOHISTOCHEMISTRY; IN VITRO STUDY; IN VIVO STUDY; NONHUMAN; PRIORITY JOURNAL; ANIMAL; CHEMISTRY; CHO CELL; CRICETULUS; ENZYME ACTIVATION; ENZYME SPECIFICITY; GENETICS; HAMSTER; HUMAN; METABOLISM; PHOSPHORYLATION; RABBIT;

CRICETULUS GRISEUS; ORYCTOLAGUS CUNICULUS;

ANIMALS; CELL MEMBRANE; CHO CELLS; CRICETINAE; CRICETULUS; CYCLIC AMP-DEPENDENT PROTEIN KINASES; ENZYME ACTIVATION; HUMANS; METHYLGLUCOSIDES; PHOSPHORYLATION; RABBITS; SODIUM-GLUCOSE TRANSPORTER 1; SUBSTRATE SPECIFICITY;

EID: 62649114642     PISSN: 07302312     EISSN: 10974644     Source Type: Journal    
DOI: 10.1002/jcb.22025     Document Type: Article

References (20)

1. Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248-254.
2. Castaneda F, Kinne RKH. 2005. A 96-well automated method to study inhibitors of human sodium-dependent D-glucose transport. Mol Cell Biochem 280:91-98.
3. Grimard V, Li C, Ramajeesingh M, Bear CE, Goormaghtigh E, Ruysschaert JM. 2004. Phosphorylation-induced conformational changes of cystic fibrosis transmembrane conductance regulator monitored by attenuated total reflection-Fourier transform 1R spectroscopy and fluorescence spectroscopy. J Biol Chem 279:5528-5536.
4. Hirsch JR, Loo DD, Wright EM. 1996. Regulation of Na+/glucose cotransporter expression by protein kinases in Xenopus laevis oocytes. J Biol Chem 271:14740-14746.
5. Ishikawa Y, Eguchi T, Ishida H. 1997. Mechanism of beta-adrenergic agonist-induced transmural transport of glucose in rat small intestine. Regulation of phosphorylation of SGLT1 controls the function. Biochim Biophys Acta 1357:306-318.
6. Johnson LN, Lewis RJ. 2001. Structural basis for control by phosphorylation. Chem Rev 101:2209-2242.
7. Khoursandi S, Scharlau D, Herter P, Kuhnen C, Martin D, Kinne RK, Kipp H. 2004. Different modes of sodium-D-glucose cotransporter-mediated D-glucose uptake regulation in Caco-2 cells. Am J Physiol Cell Physiol 287:C1041-C1047.
8. Kipp H, Khoursandi S, Scharlau D, Kinne RK. 2003. More than apical: Distribution of SGLT1 in Caco-2 cells. Am J Physiol Cell Physiol 285: C737-C749.
9. Kumar A, Tyagi NK, Goyal P, Pandey D, Siess W, Kinne RK. 2007a. Sodiumindependent low-affinity D-glucose transport by human sodium/D-glucose cotransporter 1: Critical role of tryptophan 561. Biochemistry 46:2758-2766.
10. Kumar A, Tyagi NK, Kinne RK. 2007b. Ligand-mediated conformational changes and positioning of tryptophans in reconstituted human sodium/D- glucose cotransporterl (hSGLTl) probed by tryptophan fluorescence. Biophys Chem 127:69-77.
11. Lin JT, Kormanec J, Wehner F, Wielert-Badt S, Kinne RK. 1998. High-level expression of Na+/D-glucose cotransporter (SGLT1) in a stably transfected Chinese hamster ovary cell line. Biochim Biophys Acta 1373:309-320.
12. Miranda FF, Teigen K, Thorolfsson M, Svebak RM, Knappskog PM, Flatmark T, Martinez A. 2002. Phosphorylation and mutations of Ser(16) in human phenylalanine hydroxylase. Kinetic and structural effects. J Biol Chem 277: 40937-40943.
13. Miranda FF, Thorolfsson M, Teigen K, Sanchez-Ruiz JM, Martinez A. 2004. Structural and stability effects of phosphorylation: Localized structural changes in phenylalanine hydroxylase. Protein Sci 13:1219-1226.
14. Prince LS, Tousson A, Marchase RB. 1993. Cell surface labeling of CFTR in T84 cells. Am J Physiol 264:C491-C498.
15. Raja MM, Tyagi NK, Kinne RK. 2003. Phlorizin recognition in a C-terminal fragment of SGLT1 studied by tryptophan scanning and affinity labeling. J Biol Chem 278:49154-49163.
16. Tyagi NK, Goyal P, Kumar A, Pandey D, Siess W, Kinne RK. 2005. High-yield functional expression of human sodium/d-glucose cotransporterl in Pichia pastoris and characterization of ligand-induced conformational changes as studied by tryptophan fluorescence. Biochemistry 44:15514-15524.
17. Vivian JT, Callis PR. 2001. Mechanisms of tryptophan fluorescence shifts in proteins. Biophys J 80:2093-2109.
18. Wright EM, Hager KM, Turk E. 1992. Sodium cotransport proteins. Curr Op in Cell Biol 4:696-702.
19. WrightEM, Loo DD, Turk E, Hirayama BA. 1996. Sodium cotransporters. Curr Opin Cell Biol 8:468-473.
20. Wright EM, Hirsch JR, Loo DD, Zampighi GA. 1997. Regulation of Na+/ glucose cotransporters. J Exp Biol 200:287-293.