High-yield functional expression of human sodium/D-glucose cotransporter1 in Pichia pastoris and characterization of ligand-induced conformational changes as studied by tryptophan fluorescence

Biochemistry

Volumn 44, Issue 47, 2005, Pages 15514-15524

  Tyagi, Navneet K ^a   Goyal, Pankaj ^b   Kumar, Azad ^a   Pandey, Dharmendra ^b   Siess, Wolfgang ^b   Kinne, Rolf K H ^a  

^a MAX PLANCK INSTITUTE OF MOLECULAR PHYSIOLOGY   (Germany)

^b UNIVERSITY OF MUNICH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CHROMATOGRAPHIC ANALYSIS; CONFORMATIONS; FLUORESCENCE; GLUCOSE; HYDROPHILICITY; PURIFICATION; TITRATION; VECTORS;

FUNCTIONAL EXPRESSION; HYDROPHILIC COLLISIONAL QUENCHERS; PACKING DENSITY;

PROTEINS;

8 ANILINO 1 NAPHTHALENESULFONIC ACID; ACRYLAMIDE; ALPHA METHYLGLUCOSIDE; GLUCOSE; LIGAND; NICKEL; PHLORIZIN; RECOMBINANT PROTEIN; SODIUM; SODIUM GLUCOSE COTRANSPORTER 1; TRICHLOROETHANOL; TRYPTOPHAN;

AFFINITY CHROMATOGRAPHY; ARTICLE; CATALYSIS; CLONE; COMPLEX FORMATION; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; ELECTROPORATION; EXPRESSION VECTOR; FLUORESCENCE; FUNGAL STRAIN; FUNGUS CULTURE; HYDROPHILICITY; HYDROPHOBICITY; KINETICS; MASS; NONHUMAN; PICHIA PASTORIS; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEOLIPOSOME; STEREOSPECIFICITY; SURFACE PROPERTY; TITRIMETRY;

CLONING, MOLECULAR; FLUORESCENCE; HUMANS; HYDROPHOBICITY; LIGANDS; MONOSACCHARIDES; PHLORHIZIN; PICHIA; PROTEIN CONFORMATION; SODIUM-GLUCOSE TRANSPORTER 1; SPECTROMETRY, FLUORESCENCE; SUBSTRATE SPECIFICITY; TRYPTOPHAN;

FUNGI; PICHIA; PICHIA PASTORIS;

EID: 28244499592     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi051377q     Document Type: Article

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